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Producing high titers of replication-incompetent retrovirus
Obtain retrovirus in 48 hours
Consistently high titers
Choice of envelope protein
The Retro-X Universal Packaging System provides a rapid and convenient method for producing high titers of replication-incompetent retrovirus. Choose from four different envelope proteins to package pseudotyped retrovirus with broad tropism to infect most commonly used cell types.
Retroviral gene transfer systems depend on packaging cell lines to supply necessary viral structural proteins. The provided GP2-293 cell line contains the Moloney Murine Leukemia Virus (MMLV) gag and pol genes stably integrated into its genome. Simply transfect GP2-293 cells with the retroviral vector and the appropriate viral envelope vector, wait 48 hours, and then collect your viral supernatant which is ready for transduction (Figure 1).
Produce high titer retrovirus for your cell type
Broad tropism—infect all your cells
Viral envelopes are classified by the receptors they use to enter host cells. For example, ecotropic viruses are recognized by receptors only found on mouse and rat cells. The universal packaging system includes four envelope expression vectors—ecotropic, amphotropic, dualtropic (10A1), and pantropic (VSV-G)—allowing you to select the most appropriate envelope protein to infect your cell type (Table 1).
Obtain high titers of retroviral particles
The included cell line, GP2-293, has been optimized for the production of high-titer retrovirus packaged in any of the four envelope proteins (Figure 2). Rapid and easy determination of retroviral titer may be achieved using the Retro-X qRT-PCR Titration Kit (sold separately). If desired, retroviral supernatants are easily concentrated with the Retro-X Concentrator (not shown).
The Retro-X Universal Packaging System provides a rapid and convenient method for producing high titers of replication-incompetent retrovirus. It includes four envelope expression vectors—ecotropic, amphotropic, dualtropic (10A1), and pantropic (VSV-G)—allowing you to select the most appropriate envelope protein to infect your cell type.