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Lentivirus vector selection guide
We offer a variety of highly optimized lentiviral vectors for many applications. These lentiviral vectors are key players in all of our Lenti-X gene delivery systems, which can be used with almost any mammalian cell type, including dividing and nondividing cells, primary cell cultures, stem cells, and neurons. All of our pLVX lentiviral vectors contain HIV-1 LTRs and the lentiviral packaging signal (Ψ), and we've included specific elements to improve transgene expression, virus titer, and overall lentiviral vector function:
- WPRE: A woodchuck hepatitis virus posttranscriptional regulatory element prevents poly(A) site readthrough, promotes RNA processing and maturation, and increases nuclear export of RNA. In genomic transcripts, it enhances vector packaging and increases titer. In transduced target cells, the WPRE boosts transgene expression by facilitating mRNA transcript maturation.
- cPPT/CTS: A central polypurine tract/central termination sequence creates a "DNA flap" that increases nuclear importation of the viral genome during target-cell infection. The cPPT/CTS element improves vector integration and transduction efficiency.
- RRE: A Rev response element increases titers by promoting the nuclear export of unspliced viral genomic RNA.
Click here to learn more about lentiviral vector safety.
Use these lentiviral vectors with any of our highly advanced, safe, and easy-to-use 4th-generation Lenti-X packaging systems, which produce high titers of VSV-G- or ecotropic-pseudotyped lentivirus.
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Express your gene of interest from a strong CMV promoter, and select for lentiviral integration using antibiotic selection.
For cell types where the CMV promoter gets silenced, such as hematopoietic or stem cells.
Obtain tightly controlled, inducible expression of your gene of interest with a single vector using Tet-One systems.
Clone your gene downstream of a ProteoTuner destabilization domain (DD) and control the stability of your protein with Shield1.
Clone your gene in-frame with one of our many fluorescent proteins and monitor the expression and subcellular localization of your protein.
Monitor activity of your favorite promoter using a reporter system that expresses bright fluorescent proteins or secreted luciferase.
Monitor activity of your favorite promoter using a reporter system that expresses a cyan, green, or red fluorescent protein on demand.
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