Lentivirus vector selection guide

We offer a variety of highly optimized lentiviral vectors for many applications. These lentiviral vectors are key players in all of our Lenti-X gene delivery systems, which can be used with almost any mammalian cell type, including dividing and nondividing cells, primary cell cultures, stem cells, and neurons. All of our pLVX lentiviral vectors contain HIV-1 LTRs and the lentiviral packaging signal (Ψ), and we've included specific elements to improve transgene expression, virus titer, and overall lentiviral vector function:

• WPRE: A woodchuck hepatitis virus posttranscriptional regulatory element prevents poly(A) site readthrough, promotes RNA processing and maturation, and increases nuclear export of RNA. In genomic transcripts, it enhances vector packaging and increases titer. In transduced target cells, the WPRE boosts transgene expression by facilitating mRNA transcript maturation.

• cPPT/CTS: A central polypurine tract/central termination sequence creates a "DNA flap" that increases nuclear importation of the viral genome during target-cell infection. The cPPT/CTS element improves vector integration and transduction efficiency.
• RRE: A Rev response element increases titers by promoting the nuclear export of unspliced viral genomic RNA.
  

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Use these lentiviral vectors with any of our highly advanced, safe, and easy-to-use 4^th-generation Lenti-X packaging systems, which produce high titers of VSV-G- or ecotropic-pseudotyped lentivirus.

Click the boxes below to view the vector information and map.