Retro-X Concentrator provides a fast, simple, and highly efficient method for concentrating any retroviral stock. You simply mix your retroviral supernatant with the Retro-X Concentrator reagent, incubate overnight, and spin the mixture in a standard centrifuge (Figure 1). You'll increase your vector titer by up to 100-fold and obtain excellent recoveries—without ultracentrifugation. Retro-X Concentrator works for all retroviral supernatants and pseudotypes, including those made from Takara Bio's Retro-X vectors. The procedure can be easily scaled up or down to suit your needs.
Effortless concentration of retrovirus
Retro-X Concentrator enables you to increase the available titer up to 100-fold and infect your target cells at higher MOIs, in reduced volumes, and without making more virus—all without the hassle of ultracentrifugation.
• Simply mix and spin
• Hassle-free and easily scaled up for large volumes
• Up to 100X concentration, with 90% recovery
Simple protocol: mix, wait, spin
Concentrate all common pseudotypes
Efficient concentration of retroviral particles can be achieved using the Retro-X Concentrator protocol. Retroviral titers were concentrated from 106 to 108 IFU/ml, with very high recovery in 1/100 of the original volume. Up to 100-fold concentration was achieved for particles packaged with all common envelope proteins (VSV-G, ecotropic, and amphotropic pseudotypes) when concentrating them from 10 ml down to 100 µl (Figure 2).
Learn more about our retroviral systems
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