Retro-X Concentrator provides a fast, simple, and highly efficient method for concentrating any retroviral stock. You simply mix your retroviral supernatant with the Retro-X Concentrator reagent, incubate overnight, and spin the mixture in a standard centrifuge (Figure 1). You'll increase your vector titer by up to 100-fold and obtain excellent recoveries—without ultracentrifugation. Retro-X Concentrator works for all retroviral supernatants and pseudotypes, including those made from Takara Bio's Retro-X vectors. The procedure can be easily scaled up or down to suit your needs.
Effortless concentration of retrovirus
Retro-X Concentrator enables you to increase the available titer up to 100-fold and infect your target cells at higher MOIs, in reduced volumes, and without making more virus—all without the hassle of ultracentrifugation.
• Simply mix and spin
• Hassle-free and easily scaled up for large volumes
• Up to 100X concentration, with 90% recovery
Simple protocol: mix, wait, spin
Concentrate all common pseudotypes
Efficient concentration of retroviral particles can be achieved using the Retro-X Concentrator protocol. Retroviral titers were concentrated from 106 to 108 IFU/ml, with very high recovery in 1/100 of the original volume. Up to 100-fold concentration was achieved for particles packaged with all common envelope proteins (VSV-G, ecotropic, and amphotropic pseudotypes) when concentrating them from 10 ml down to 100 µl (Figure 2).
Far simpler than ultracentrifugation
In a side-by-side comparison between Retro-X Concentrator and ultracentrifugation, the advantages of Retro-X are clearly evident (Table I). Retro-X Concentrator is more flexible, faster, easier, and just as efficient as ultracentrifugation.
Table I. Retro-X Concentrator vs. ultracentrifugation
|Table I. Retro-X Concentrator vs. ultracentrifugation|
|Centrifugation time required||45 min||4 hr to overnight|
Learn more about our retroviral systems
Retroviral product selection guide
A selection guide to help you choose the best retroviral vectors, packaging systems, and other tools.
Universal packaging system
Versatile packaging system for producing high titers of virus with a choice of commonly used envelopes.
Increase your viral titer up to 100-fold with Retro-X Concentrator—without ultracentrifugation.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.