Effortless concentration of retrovirus

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Viral transduction

Product Overview

Retro-X Concentrator enables you to increase the available titer up to 100-fold and infect your target cells at higher MOIs, in reduced volumes, and without making more virus—all without the hassle of ultracentrifugation.

• Simply mix and spin
• Hassle-free and easily scaled up for large volumes
• Up to 100X concentration, with 90% recovery

Simple protocol: mix, wait, spin Concentrate all common pseudotypes Far simpler than ultracentrifugation

Simple protocol: mix, wait, spin

Retro-X Concentrator provides a fast, simple, and highly efficient method for concentrating any retroviral stock. You simply mix your retroviral supernatant with the Retro-X Concentrator reagent, incubate overnight, and spin the mixture in a standard centrifuge (Figure 1). You'll increase your vector titer by up to 100-fold and obtain excellent recoveries—without ultracentrifugation. Retro-X Concentrator works for all retroviral supernatants and pseudotypes, including those made from Takara Bio's Retro-X vectors. The procedure can be easily scaled up or down to suit your needs.

Concentrate all common pseudotypes

Efficient concentration of retroviral particles can be achieved using the Retro-X Concentrator protocol. Retroviral titers were concentrated from 10⁶ to 10⁸ IFU/ml, with very high recovery in 1/100 of the original volume. Up to 100-fold concentration was achieved for particles packaged with all common envelope proteins (VSV-G, ecotropic, and amphotropic pseudotypes) when concentrating them from 10 ml down to 100 µl (Figure 2).

Far simpler than ultracentrifugation

In a side-by-side comparison between Retro-X Concentrator and ultracentrifugation, the advantages of Retro-X are clearly evident (Table I). Retro-X Concentrator is more flexible, faster, easier, and just as efficient as ultracentrifugation.

Table I. Retro-X Concentrator vs. ultracentrifugation

Table I. Retro-X Concentrator vs. ultracentrifugation
Feature	Retro-X Concentrator	Ultracentrifugation
Easily scalable	Yes	No
Specialized equipment	No	Yes
Centrifugation time required	45 min	4 hr to overnight
Ease-of-use	High	Low
Yield	>90%	>90%

Related Products

Cat. #	Product	Size	Price	Quantity
631455	Retro-X™ Concentrator	100 mL	$214.00	*
The Retro-X Concentrator is a complete reagent for the concentration of infectious retroviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes and envelope used. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Simple method to concentrate retrovirus Simple method to concentrate retrovirus. Add Retro-X Concentrator reagent to clarified viral supernatant, incubate overnight at 4°C, and spin. That’s it. Back Far simpler than ultracentrifugation Far simpler than ultracentrifugation. In a side-by-side comparison of Retro-X Concentrator and ultracentrifugation, the advantages of the Retro-X method are clearly evident. Retro-X Concentrator is more flexible and easier to use than ultracentrifugation. The Retro-X Concentrator reagent is itself a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. Scalability has been successful for supernatant volumes up to 250 ml. Back Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes. A retrovirus expressing DsRed2 was packaged with different retroviral envelopes using the Retro-X Universal Packaging System (Cat. # 631530), and harvested at 72 hr posttransfection. Retro-X Concentrator was used to concentrate dualtropic (10A1), amphotropic (Ampho), ecotropic (Eco), and VSV-G (pantropic) pseudotyped retrovirus samples from 10 ml down to 100 µl (Panels A & B). Crude and concentrated viral stocks were then titrated on HT1080 or NIH 3T3 cells and analyzed via flow cytometry at 72 hr postinfection to determine the percentage of positive cells. Back Retro-X Concentrator Catalog No.631455 Retro-X Concentrator Catalog No.631455.
631456	Retro-X™ Concentrator	500 mL	$735.00	*
The Retro-X Concentrator is a complete reagent for the concentration of infectious retroviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes and envelope used. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Simple method to concentrate retrovirus Simple method to concentrate retrovirus. Add Retro-X Concentrator reagent to clarified viral supernatant, incubate overnight at 4°C, and spin. That’s it. Back Far simpler than ultracentrifugation Far simpler than ultracentrifugation. In a side-by-side comparison of Retro-X Concentrator and ultracentrifugation, the advantages of the Retro-X method are clearly evident. Retro-X Concentrator is more flexible and easier to use than ultracentrifugation. The Retro-X Concentrator reagent is itself a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. Scalability has been successful for supernatant volumes up to 250 ml. Back Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes. A retrovirus expressing DsRed2 was packaged with different retroviral envelopes using the Retro-X Universal Packaging System (Cat. # 631530), and harvested at 72 hr posttransfection. Retro-X Concentrator was used to concentrate dualtropic (10A1), amphotropic (Ampho), ecotropic (Eco), and VSV-G (pantropic) pseudotyped retrovirus samples from 10 ml down to 100 µl (Panels A & B). Crude and concentrated viral stocks were then titrated on HT1080 or NIH 3T3 cells and analyzed via flow cytometry at 72 hr postinfection to determine the percentage of positive cells. Back Retroviral vectors available from Clontech Retroviral vectors available from Takara Bio. Not all vectors are shown. Some retroviral vectors are not sold separately. Back Retro-X Concentrator Cat. # 631456 Retro-X Concentrator. Cat. # 631456.

*You must be logged in to a Business Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

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Universal packaging system

Versatile packaging system for producing high titers of virus with a choice of commonly used envelopes.

Retro-X Concentrator

Increase your viral titer up to 100-fold with Retro-X Concentrator—without ultracentrifugation.

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