Lentiviral product selection guide
Use the list below to choose the right lentiviral product for your research:
Lentiviral vectors
Lentiviral vector selection guide
Learn more about the core elements included in all of our lentiviral vectors and see schematics of each vector type.
EF-1 alpha promoter vectors
Express your gene of interest from an EF-1 alpha promoter in all cell types, including those in which CMV promoters are often silenced, such as hematopoietic cells and stem cells.
All-in-one tetracycline-inducible expression systems
Single-vector systems to easily generate inducible expression constructs with high-fold induction.
Vectors to control protein levels (ProteoTuner)
Clone your gene downstream of a ProteoTuner destabilization domain (DD) and rapidly control the level of your protein with Shield1.
Fluorescent protein expression vectors
Monitor expression and transduction efficiency with bright red, cyan, and green fluorescent proteins.
IRES bicistronic vectors
Coexpress your transgene and an antibiotic or fluorescent marker using IRES-containing lentiviral vector systems.
Inducible protein dimerization vectors (iDimerize)
Fuse DmrB domains to your protein(s) of interest and induce homo- or heterodimerization with dimerizing ligands.
Promoterless reporter vectors (fluorescent protein)
Monitor activity of your favorite promoter using a destabilized cyan, green, or red fluorescent protein.
Learn more »
Lentiviral packaging systems and cell line
High-titer lentiviral packaging system—pantropic (VSV-G) pseudotype
Obtain titers >107 IFU/ml. If you are not currently getting at least 107 IFU/ml, you need Lenti-X Packaging Single Shots.
High-titer lentiviral ecotropic packaging system
Get the same high titers, but with virus that has an ecotropic envelope. This limits transduction to murine cells or human cells manipulated to express the mCAT1 receptor.
Integrase-deficient lentiviral packaging system
Produce lentivirus that does not integrate into the target-cell genome.
Lenti-X 293T cell line
Not all 293T cell lines are created equal. This one was clonally selected to produce high lentiviral titers.
Titration kits
Ten-minute titer test
Decide if there is enough lentivirus in your culture supernatant using a simple kit that works like an at-home ELISA test.
ELISA titration kit
Measure the amount of p24 capsid protein present in your viral supernatant and correlate it directly with lentivirus titer.
qRT-PCR titration kit
Use a fast and simple quantitative RT-PCR kit to measure lentivirus titer.
Integrated provirus quantitation kit
Determine how many lentiviral genomes have integrated into your target cells (e.g., the copy number).
Concentration reagent
Lenti-X Concentrator
Increase lentivirus titer 100X—no ultracentrifugation. Just mix your lentiviral supernatant with the lentivirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
Transduction enhancers
Magnetic transduction beads
Perform lentiviral transduction in only 30 min using the magnetic beads supplied with the Lenti-X Accelerator. Simply bind your viral supernatant to the beads and pull lentivirus or retrovirus to your cells with a magnet.
RetroNectin reagent
Coat your culture dish with this multivalent molecule to significantly increase transduction efficiency. RetroNectin co-localizes lentivirus (or retrovirus) particles with your target cells. Perfect for hard-to-transduce suspension cells and stem cells.
Ecotropic Receptor Booster
Efficiently transduce human cells with ecotropic lentivirus or retrovirus that otherwise could only infect rodent cells. The booster consists of concentrated exosome-like vesicles that are densely coated with the mCAT-1 receptor protein, the target for ecotropic viruses.
Purification kit
Lenti-X Maxi Purification Kit
Simple—just like plasmid maxipreps. A gentle gravity flow-based purification procedure is the key to maintaining viral infectivity and producing excellent lentivirus yields of up to 80%.
Related product pages
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