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Lentivirus

Ecotropic Receptor Booster

Temporarily elevate the density of the ecotropic receptor protein mCAT-1 on the surface of any target cell that you wish to infect

The Ecotropic Receptor Booster is used to temporarily elevate the density of the ecotropic receptor protein mCAT-1 on the surface of any target cell that you wish to infect. This allows you, for example, to efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise could only infect rodent cells. The technology also allows you to increase the infection efficiency of any cell type that is resistant to viral infection.

The following products are recommended for producing ecotropic pseudotyped virus:

Ecotropic lentivirus—Lenti-X Packaging Single Shots (Ecotropic)
Ecotropic retrovirus—Retro-X Universal Packaging System or EcoPack 2-293 Cell Line

How does it work?

The Ecotropic Receptor Booster consists of a vial of concentrated exosome-like vesicles that are densely coated with the mCAT-1 receptor protein (expand the Details tab in the product table below to view data images). When the booster vesicles are applied to your cells (10–20 µl per well of a 6-well plate, for ~2 hr), they fuse with the plasma membrane, resulting in an increased level of the receptor on the cell surface. Your target cells can then be more efficiently transduced with ecotropic pseudotyped lentivirus or retrovirus.

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Cat. #

Product

Size

License

Quantity

Details

631471

Ecotropic Receptor Booster

20 Rxns

USD $323.00

License Statement

ID Number

225

Gesicle Technology. This product and its use are the subject of European patents and patent applications related to EP09306091, owned by Inserm Transfert. For research use only – not for therapeutic or diagnostic use in humans. No rights granted other that the right to use for research purposes.

In addition, Inserm Transfert is the owner of European patent application EP09306092.9 relating to the use of microvesicles for cell engineering purposes. The products should not be used to develop, make, have made, use and sell or otherwise distribute any product, composition, method, service or process for commercial services that would infringe said patent application, unless a license is negotiated with Inserm Transfert, at 7 rue Watt, 75013 Paris, France (www.inserm-transfert.fr).

The Ecotropic Receptor Booster increases the transduction efficiency of cells that are either resistant to infection, or inefficiently infected by ecotropic pseudotyped lentivirus or retrovirus. The Ecotropic Receptor Booster consists of exosome-like particles coated with the mCAT-1 receptor. When applied to target cells, fusion of the exosome-like particles with the host cell plasma membrane temporarily increases surface mCAT-1 receptor, thus allowing for efficient viral transduction.The Ecotropic Receptor Booster renders cells that cannot usually be transduced with ecotropic psuedotyped virus (e.g. human celllines) susceptible to viral infection.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

Required Products

Cat. #

Product

Size

Price

License

Quantity

Details

631530

Retro-X™ Universal Packaging System

Each

USD $897.00

License Statement

ID Number
8	For Research Use Only, Not for Use in Humans.

This kit provides a complete retroviral vector packaging system for use with any retroviral vector. It is designed to produce virus that can infect dividing cells from a broad range of mammalian and nonmammalian cell types. The GP2-293 Packaging Cell Line contains only the MoMuLV gag and pol genes. The viral envelope portion of the packaging function (env gene) is supplied by transiently co-transfecting one of the Retro-X Universal Packaging Vectors (p10A1, pAmpho, pEco, or pVSV-G) with the retroviral vector. The pQCLIN Retroviral Vector (included in the vector set) can be used as a positive control to constitutively express β-galactosidase in transduced mammalian cells.

Components

631251

Lenti-X™ HTX Ecotropic Packaging System

20 Rxns

USD $1076.00

License Statement

ID Number

Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 6,998,115; 7,427,394. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.

This product is protected by U.S. Patent 9,945,850 and additional pending patent. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The Lenti-X HTX Ecotropic Packaging System allows you to produce very high virus titers from lentiviral vectors for the purposes of transferring exogenous genes into cells and tissues. The Lenti-X HTX Ecotropic Packaging Mix 2 is a proprietary combination of plasmids that expresses the viral proteins necessary for high-efficiency packaging of lentiviral vector transcripts into infectious, ecotropically-pseudotyped, lentiviral particles. The MLV ecotropic envelope glycoprotein enables mouse and rat cells to be transduced with high efficiency. The biodegradable nanoparticle-based Xfect transfection reagent transfers the packaging mix DNA and your lentiviral vector into Lenti-X 293T cells (Cat. No. 632180) with very high efficiency. It is the specific combination of these rigorously optimized components that allows your 293T cell cultures to produce extremely high titers of safe, replication-incompetent lentivirus from any of our Lenti-X System vectors, or from any other lentiviral vector. The Lenti-X GoStix can be used to determine whether virus production is within a usable range or for selecting the best time to harvest your virus.

Components

Overview

Transduce any cell type with ecotropic lentivirus or retrovirus, including human cells
High infection efficiency regardless of the cell line transduced
Increase efficiency of transduction by temporarily increasing the concentration of viral receptor proteins on your target cells
Simply preincubate your target cells with these concentrated exosome-like vesicles for 2 hr prior to infection with virus
The Ecotropic Receptor Booster delivers a high density of the mCAT-1 receptor to your target cells

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Ecotropic Receptor Booster

How does it work?

Notice to purchaser

Receptor boosters are concentrated exosome-like vesicles that are applied to your target cells prior to infection with virus

Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used

Usually, it is not possible to infect Jurkat cells, human mesenchymal stem cells, or human primary neural progenitor cells with ecotropic pseudotyped lentivirus

631471: Ecotropic Receptor Booster

Schematic for retroviral packaging using Retro-X Universal Packaging System

Retrovirus packaging options from Clontech

Characteristics of retroviral packaging cell lines: AmphoPack-293, EcoPack 2-293, and RetroPack PT67

High titer retroviral packaging with a choice of four different retroviral packaging envelopes

Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used

Clontech's lentiviral packaging system (Panel A) and a lentiviral packaging system from a leading competitor (Panel B) were each used to generate viral supernatants from their respective lentiviral system vectors that were engineered to express the ZsGreen1 fluorescent protein

Transduction of neural progenitor cells by Lenti-X lentivirus

Overview

More Information