We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us

Close

  • ‹ Back to Transduction enhancers
  • Lenti-X Accelerator
  • Ecotropic Receptor Booster
Selection guides Virus kits: product finder tool
Gene function learning center Gene function learning center
Home › Products › Gene function › Viral transduction › Lentivirus › Transduction enhancers › Ecotropic Receptor Booster

Lentivirus

  • Vector systems
    • Constitutive promoter
    • EF-1 alpha promoter
    • IRES bicistronic
    • Fluorescent protein
    • Tet-inducible
  • Premade lentiviral particles
    • Whole-cell labeling
    • Organelle labeling
    • Tet-On 3G transactivator
  • Packaging systems and cells
    • Lenti-X packaging single shots
    • Lenti-X 293T cells
  • Titration kits
    • Lenti-X GoStix Plus
    • qRT-PCR
    • p24 ELISA
    • Integrated copy number
  • Transduction enhancers
    • Lenti-X Accelerator
    • Ecotropic Receptor Booster
Need help?
Contact Sales
Selection guides Virus kits: product finder tool
Gene function learning center Gene function learning center

Ecotropic Receptor Booster

Temporarily elevate the density of the ecotropic receptor protein mCAT-1 on the surface of any target cell that you wish to infect

The Ecotropic Receptor Booster is used to temporarily elevate the density of the ecotropic receptor protein mCAT-1 on the surface of any target cell that you wish to infect. This allows you, for example, to efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise could only infect rodent cells. The technology also allows you to increase the infection efficiency of any cell type that is resistant to viral infection.

The Ecotropic Receptor Booster is used to temporarily elevate the density of the ecotropic receptor protein mCAT-1 on the surface of any target cell that you wish to infect. This allows you, for example, to efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise could only infect rodent cells. The technology also allows you to increase the infection efficiency of any cell type that is resistant to viral infection.

The following products are recommended for producing ecotropic pseudotyped virus:

  • Ecotropic lentivirus—Lenti-X Packaging Single Shots (Ecotropic)
  • Ecotropic retrovirus—Retro-X Universal Packaging System  or EcoPack 2-293 Cell Line

How does it work?

The Ecotropic Receptor Booster consists of a vial of concentrated exosome-like vesicles that are densely coated with the mCAT-1 receptor protein (expand the Details tab in the product table below to view data images). When the booster vesicles are applied to your cells (10–20 µl per well of a 6-well plate, for ~2 hr), they fuse with the plasma membrane, resulting in an increased level of the receptor on the cell surface. Your target cells can then be more efficiently transduced with ecotropic pseudotyped lentivirus or retrovirus.

 More  Less
Cat. # Product Size License Quantity Details
631471 Ecotropic Receptor Booster 20 Rxns USD $323.00

License Statement

ID Number  
225 Gesicle Technology. This product and its use are the subject of European patents and patent applications related to EP09306091, owned by Inserm Transfert. For research use only – not for therapeutic or diagnostic use in humans. No rights granted other that the right to use for research purposes.

In addition, Inserm Transfert is the owner of European patent application EP09306092.9 relating to the use of microvesicles for cell engineering purposes. The products should not be used to develop, make, have made, use and sell or otherwise distribute any product, composition, method, service or process for commercial services that would infringe said patent application, unless a license is negotiated with Inserm Transfert, at 7 rue Watt, 75013 Paris, France (www.inserm-transfert.fr).

The Ecotropic Receptor Booster increases the transduction efficiency of cells that are either resistant to infection, or inefficiently infected by ecotropic pseudotyped lentivirus or retrovirus. The Ecotropic Receptor Booster consists of exosome-like particles coated with the mCAT-1 receptor. When applied to target cells, fusion of the exosome-like particles with the host cell plasma membrane temporarily increases surface mCAT-1 receptor, thus allowing for efficient viral transduction.The Ecotropic Receptor Booster renders cells that cannot usually be transduced with ecotropic psuedotyped virus (e.g. human celllines) susceptible to viral infection.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

Receptor boosters are concentrated exosome-like vesicles that are applied to your target cells prior to infection with virus

Receptor boosters are concentrated exosome-like vesicles that are applied to your target cells prior to infection with virus

Receptor boosters are concentrated exosome-like vesicles that are applied to your target cells prior to infection with virus. Boosters result in increased transduction efficiency because treatment increases the cell surface density of the receptor recognized by the lentivirus (ecotropic) or retrovirus (ecotropic or amphotropic). Using this technology, for example, you can coat human cells with the ecotropic receptor (which is otherwise absent), enabling them to be transduced with ecotropic retrovirus or ecotropic lentivirus.

Back

Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used

Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used
Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used. Panel A. HT1080 cells were plated in 6-well plates 24 hr prior to transduction and incubated with 10 µl Ecotropic Receptor Booster for 2 hr. Cells were then transduced with Lenti-X ZsGreen1 lentivirus (MOI=~15) produced using Clontech's Lenti-X HTX Ecotropic Packaging System and assayed 48 hr later for ZsGreen1 expression. Panel B. A variety of human cell types that are normally resistant to transduction by ecotropic-pseudotyped lentiviral vectors were rendered susceptible to transduction by pre-treatment with the Ecotropic Receptor Booster.

Back

Usually, it is not possible to infect Jurkat cells, human mesenchymal stem cells, or human primary neural progenitor cells with ecotropic pseudotyped lentivirus

Usually, it is not possible to infect Jurkat cells, human mesenchymal stem cells, or human primary neural progenitor cells with ecotropic pseudotyped lentivirus
Usually, it is not possible to infect Jurkat cells, human mesenchymal stem cells, or human primary neural progenitor cells with ecotropic pseudotyped lentivirus. However, pre-treatment of these cell types with Ecotropic Receptor Booster allows for very efficient transduction.

Back

631471: Ecotropic Receptor Booster

631471: Ecotropic Receptor Booster

Required Products

Cat. # Product Size Price License Quantity Details
631530 Retro-X™ Universal Packaging System Each USD $897.00

License Statement

ID Number  
8 For Research Use Only, Not for Use in Humans.

This kit provides a complete retroviral vector packaging system for use with any retroviral vector. It is designed to produce virus that can infect dividing cells from a broad range of mammalian and nonmammalian cell types. The GP2-293 Packaging Cell Line contains only the MoMuLV gag and pol genes. The viral envelope portion of the packaging function (env gene) is supplied by transiently co-transfecting one of the Retro-X Universal Packaging Vectors (p10A1, pAmpho, pEco, or pVSV-G) with the retroviral vector. The pQCLIN Retroviral Vector (included in the vector set) can be used as a positive control to constitutively express β-galactosidase in transduced mammalian cells.

Documents Components You May Also Like Image Data

Back

Schematic for retroviral packaging using Retro-X Universal Packaging System

Schematic for retroviral packaging using Retro-X Universal Packaging System
Schematic for retroviral packaging using Retro-X Universal Packaging System.

Back

Retrovirus packaging options from Clontech

Retrovirus packaging options from Clontech
Retrovirus packaging options from Clontech. Summary of the cell lines and tropisms associated with commonly used retroviral envelopes.

Back

Characteristics of retroviral packaging cell lines: AmphoPack-293, EcoPack 2-293, and RetroPack PT67

Characteristics of retroviral packaging cell lines: AmphoPack-293, EcoPack 2-293, and RetroPack PT67
Characteristics of retroviral packaging cell lines: AmphoPack-293, EcoPack 2-293, and RetroPack PT67.

Back

High titer retroviral packaging with a choice of four different retroviral packaging envelopes

High titer retroviral packaging with a choice of four different retroviral packaging envelopes

High titer retroviral packaging with a choice of four different retroviral packaging envelopes. GP2-293 Cells were transfected with a Retro-X Q vector expressing the red fluorescent protein DsRed2, and each of the four envelope vectors supplied with the Retro-X Unversal Packaging System. After incubating the transfected GP2-293 cells for 48 hr, 10 ?l of the resulting virus was used to infect NIH 3T3 cells. After 48 hours, titers were measured by flow cytometry. Titers were calculated by multiplying the percent infected cells (infectious units) by the total cell number, then dividing by the volume of virus (ml). Panel A. Ecotropic envelope. Panel B. Amphotropic envelope. Panel C. Dualtropic envelope. Panel D. VSV-G envelope (pantropic). Positive cells are indicated by the red line. Negative control cells are indicated by the green line.

631251 Lenti-X™ HTX Ecotropic Packaging System 20 Rxns USD $1076.00

License Statement

ID Number  
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 6,998,115; 7,427,394. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
89 This product is protected by U.S. Patent 9,945,850 and additional pending patent. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The Lenti-X HTX Ecotropic Packaging System allows you to produce very high virus titers from lentiviral vectors for the purposes of transferring exogenous genes into cells and tissues. The Lenti-X HTX Ecotropic Packaging Mix 2 is a proprietary combination of plasmids that expresses the viral proteins necessary for high-efficiency packaging of lentiviral vector transcripts into infectious, ecotropically-pseudotyped, lentiviral particles. The MLV ecotropic envelope glycoprotein enables mouse and rat cells to be transduced with high efficiency. The biodegradable nanoparticle-based Xfect transfection reagent transfers the packaging mix DNA and your lentiviral vector into Lenti-X 293T cells (Cat. No. 632180) with very high efficiency. It is the specific combination of these rigorously optimized components that allows your 293T cell cultures to produce extremely high titers of safe, replication-incompetent lentivirus from any of our Lenti-X System vectors, or from any other lentiviral vector. The Lenti-X GoStix can be used to determine whether virus production is within a usable range or for selecting the best time to harvest your virus.

Documents Components You May Also Like Image Data

Back

Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used

Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used
Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used. Panel A. HT1080 cells were plated in 6-well plates 24 hr prior to transduction and incubated with 10 µl Ecotropic Receptor Booster for 2 hr. Cells were then transduced with Lenti-X ZsGreen1 lentivirus (MOI=~15) produced using Clontech's Lenti-X HTX Ecotropic Packaging System and assayed 48 hr later for ZsGreen1 expression. Panel B. A variety of human cell types that are normally resistant to transduction by ecotropic-pseudotyped lentiviral vectors were rendered susceptible to transduction by pre-treatment with the Ecotropic Receptor Booster.

Back

Clontech's lentiviral packaging system (Panel A) and a lentiviral packaging system from a leading competitor (Panel B) were each used to generate viral supernatants from their respective lentiviral system vectors that were engineered to express the ZsGreen1 fluorescent protein

Clontech's lentiviral packaging system (Panel A) and a lentiviral packaging system from a leading competitor (Panel B) were each used to generate viral supernatants from their respective lentiviral system vectors that were engineered to express the ZsGreen1 fluorescent protein
Clontech's lentiviral packaging system (Panel A) and a lentiviral packaging system from a leading competitor (Panel B) were each used to generate viral supernatants from their respective lentiviral system vectors that were engineered to express the ZsGreen1 fluorescent protein. As little as 10 µl of supernatant from Lenti-X transduced the majority of these HeLa cells, whereas 10 µl of supernatant from the other system transduced only a small percentage of the cells. Transduced cells were quantified by flow cytometry.

Back

Transduction of neural progenitor cells by Lenti-X lentivirus

Transduction of neural progenitor cells by Lenti-X lentivirus

Transduction of neural progenitor cells by Lenti-X lentivirus. Recombinant lentivirus for expressing ZsGreen1 was produced using Lenti-X virus and used to transduce normal human neural progenitor cells. A single transduced cell is shown under phase contrast microscopy (Panel A) and fluorescence microscopy (Panel B).

RetroNectin reagent RetroNectin resources
Overviews Lenti-X Concentrator overview

Overview

  • Transduce any cell type with ecotropic lentivirus or retrovirus, including human cells
  • High infection efficiency regardless of the cell line transduced
  • Increase efficiency of transduction by temporarily increasing the concentration of viral receptor proteins on your target cells
  • Simply preincubate your target cells with these concentrated exosome-like vesicles for 2 hr prior to infection with virus
  • The Ecotropic Receptor Booster delivers a high density of the mCAT-1 receptor to your target cells

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2023 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • OEM
  • Capabilities and installations
  • OEM enzyme FAQs
  • Enzyme samples for commerical assay developers
  • OEM process
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Partnering & Licensing
  • Vector information
  • Vector document overview
  • Vector document finder
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • cDNA synthesis
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • Antibodies and ELISA
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • Announcements
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Trademarks
  • License statements
  • Quality statement
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Website FAQs

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • COVID-19 research
  • Next-generation sequencing
  • Diagnostic solutions
  • Real-time PCR
  • Stem cell research
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Nucleic acid purification
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • New products
  • Special offers
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • Services & Support
  • OEM
  • Instrument services
  • Stem cell services
  • Gene and cell therapy manufacturing
  • Customer service
  • Sales
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • Partnering & Licensing
  • Vector information
  • OEM
  • Capabilities and installations
  • OEM enzyme FAQs
  • Enzyme samples for commerical assay developers
  • OEM process
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Online tools
  • GoStix Plus FAQs
  • Vector information
  • Vector document overview
  • Vector document finder
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • cDNA synthesis
  • Real-time PCR
  • Nucleic acid purification
  • PCR
  • Cloning
  • Stem cell research
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • APPLICATIONS
  • Molecular diagnostics
  • Vaccine development
  • Pathogen detection
  • Immunotherapy research
  • Cancer research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • Announcements
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • Takara Bio affiliates & distributors
  • Need help?
  • Website FAQs
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us