Lenti-X transduction sponges
Lenti-X Transduction Sponge (Cat. # 631478) is a novel technology that achieves microfluidics-driven lentiviral transduction efficiency gains without the use of microfluidics-based instrumentation. With one sponge, you can transduce 105–107 cells without the need for time-consuming spinoculation or harsh chemical transduction enhancers that require optimization to retain cell viability. The Lenti‑X Transduction Sponge enables you to effectively transduce a wide variety of cell types, including cell lines and difficult-to-transduce primary cells.
Lenti‑X T‑Cell Transduction Sponge (Cat. # 631480) is a targeted variation of the Lenti‑X Transduction Sponge. The Lenti-X T‑Cell Transduction Sponge comes infused with an optimized blend of rhIL-2 and anti-human CD3 and CD28 antibodies. The simple workflow enables customization to add your desired cytokine in addition to rhIL‑2. T‑cell activation and transduction are performed in a single step, conveniently bypassing the T‑cell activation step, for an efficient, streamlined T‑cell transduction solution.
Overview of the Lenti‑X transduction sponges. Panel A. The alginate-based sponges are packaged in a sturdy blister pack to prevent crushing the microporous structure. Panel B. Illustration depicting how the microporous structure enables colocalization of cells and viruses, promoting increased transduction efficiency. Panel C. (Left) The Lenti‑X Transduction Sponge. (Right) The Lenti‑X T-Cell Transduction Sponge, based on the original transduction sponge, comes preinfused with two anti-human antibodies and IL‑2 cytokine.
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