High-titer, one-step lentivirus packaging systems
Lenti-X packaging single shots are fourth-generation lentiviral packaging systems utilizing an extremely simple, consistent one-step method for producing high-titer lentivirus. Typically, titers of 107–108 IFU per ml can be expected for the VSV-G version.
No additional transfection reagent is needed. Lenti-X packaging single shots consist of optimized ratios of Xfect Transfection Reagent and Lenti-X packaging plasmids—lyophilized and pre-aliquoted in single tubes or 96-well plates. High titer virus is produced by simply reconstituting this mixture with your lentiviral vector of choice in sterile water and adding it to 293T cells, e.g., Lenti-X 293T Cell Line (Cat. # 632180).
Lenti-X packaging single shots are fourth-generation lentiviral packaging systems utilizing an extremely simple, consistent one-step method for producing high-titer lentivirus. Typically, titers of 107–108 IFU per ml can be expected for the VSV-G version.
No additional transfection reagent is needed. Lenti-X packaging single shots consist of optimized ratios of Xfect Transfection Reagent and Lenti-X packaging plasmids—lyophilized and pre-aliquoted in single tubes or 96-well plates. High titer virus is produced by simply reconstituting this mixture with your lentiviral vector of choice in sterile water and adding it to 293T cells, e.g., Lenti-X 293T Cell Line (Cat. # 632180).
Choose from four types of Lenti-X packaging single shots optimized for different tropisms and integration properties:
- Lenti-X Packaging Single Shots (VSV-G) and Lenti-X Packaging Single Shots (96-well, VSV-G) provide the highest titers (107–108 IFU per ml), give the broadest tropism, and are provided in single-tube and 96-well formats, respectively
- Lenti-X Packaging Single Shots (Ecotropic) restrict tropism to cells of mouse and rat origin
- Lenti-X Packaging Single Shots (Integrase Deficient) produce VSV-G pseudotyped lentivirus that has a significantly reduced ability to integrate into the host cell genome
- Lenti-X Packaging Single Shots (Envelope-Free) enable the flexiblity to produce lentivirus pseudotyped with an envelope protein of choice
The provided amounts of reagent and vector are optimized for high-titer lentivirus production in either a 10-cm dish or 96-well plate. Transfections can be carried out entirely in the presence of serum. Use of tetracycline-free FBS is critical for achieving high titers with this technology.
Overview
- Fourth-generation lentiviral packaging system combines unparalleled convenience with high performance
- Simple protocol—just add your lentiviral DNA, mix, and add to 293T cells
NOTE: an additional cloning step may required for Lenti-X Packaging Single Shots (Envelope-Free) - No optimization or additional transfection reagent required
- Individual tubes and 96-well plates contain lyophilized transfection reagents (Xfect polymer and buffer) and lentivirus packaging plasmids
- Simple protocol—just add your lentiviral DNA, mix, and add to 293T cells
- The provided amounts of reagent and vector are optimized for high-titer lentivirus production in either a 10-cm dish or 96-well plate
- Choice of three types of Lenti-X packaging single shots optimized for VSV-G pseudotyped, ecotropic, or integrase-deficient lentivirus
- Flexibility to produce lentivirus pseudotyped with your envelope protein of choice
- Consistently high titers (107–108 IFU/ml)
- 96-well format available for high-throughput applications
More Information
Applications
- Gene delivery to hard-to-transfect cell types
- Creation of stable cell lines
- Streamlined creation of VSV-G pseudotyped, ecotropic, or integrase-deficient lentivirus
- Creation of lentivirus pseudotyped with an envelope protein of choice for use in serological assays
- High-throughput production of lentivirus for CRISPR or shRNA screening and vector optization workflows
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Lenti-X Packaging Single Shots
Watch the video and see how easy it is to use Lenti-X Packaging Single Shots to obtain consistently high lentiviral titers.
Video—fourth-generation lentiviral packaging
Generate lentiviral titers that far exceed most other commercially available packaging systems—you can expect titers of 107–108 infectious units (IFU) per ml, far higher than other popular systems generate. Watch the video in the first tab to find out how our fourth-generation packaging system can generate such high titers.
Learn about Lenti-X packaging Lentiviral workflowTakara Bio USA, Inc.
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