Lentiviral particles for fluorescent labeling of specific subcellular structures
These premade, highly concentrated and purified lentiviral particles deliver sequences that express fluorescent protein tags (AcGFP1 or mCherry) targeted to specific subcellular locations. Particles are available for visualization of the nucleus, endoplasmic reticulum, mitochondria, endosomes, golgi, cytoskeleton, and plasma membrane
These premade, highly concentrated and purified lentiviral particles deliver sequences that express fluorescent protein tags (AcGFP1 or mCherry) targeted to specific subcellular locations. Particles are available for visualization of the nucleus, endoplasmic reticulum, mitochondria, endosomes, golgi, cytoskeleton, and plasma membrane. Transduction allows direct visualization of specific organelles or structures, creating reporter cells that are useful for investigating protein localization, protein dynamics, and protein trafficking.
The fluorescent markers offered (AcGFP1 and mCherry) allow for multicolor labeling and direct detection by fluorescence microscopy.
Overview
- What does 109 TU/ml mean?
- In each microliter, there will be at least 1 x 106 functional lentivirus particles.
- Each 60-µl kit is sufficient for 30 transductions (using an MOI of 10) in a 6-well plate containing 2 x 105 cells/well.
- Ready-to-use particles can be added directly to cells:
- Highly concentrated lentiviral particles; all lots are >109 transducing units (TU) per ml (functional titer, not a just a particle count).
- High-purity lentiviral particles—transduce cells without cytotoxicity.
- Expression can be monitored in living cells by fluorescence microscopy
- AcGFP1 is a true monomeric green fluorescent protein with spectral properties similar to those of EGFP.
- mCherry is the most widely adopted monomeric red fluorescent protein.
- Learn more about the properties of these fluorescent proteins.
- Particles are available for labeling the nucleus, endoplasmic reticulum, actin, mitochondria, tubulin, endosome, golgi, and plasma membrane.
- These vectors have a puromycin resistance cassette and the stable clones integrating these constructs can be selected using puromycin.
More Information
Applications
- Cell organelle/structure labeling—monitor protein localization, protein trafficking, organelle dynamics, etc.
- Simple method for generating stable reporter cell lines
Additional product information
Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Webinar: highly concentrated and pure lentiviral particle preps
Watch our webinar to learn all about genome editing with lentiviral particles. Topics covered include: how to produce high-quality and high-titer lentivirus, the differences between lentiviral production systems, and applications such as the use of lentivirus for tet-inducible gene expression or subcellular localization studies.
Webinar Technology overviewTakara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2026 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
