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Home › Products › Gene function › Viral transduction › Lentivirus › Premade lentiviral particles › Whole-cell labeling

Lentivirus

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Selection guides Virus kits: product finder tool
Resources to learn about viral transduction tools Viral transduction learning center

Lentiviral particles for fluorescent whole-cell labeling

Fluorescent premade lentivirus for whole-cell labeling

Cells transduced with these premade, super-high titer particles will express a bright fluorescent protein in one of four colors: red (TdTomato or mCherry), green (ZsGreen1), cyan (AmCyan1), or yellow (ZsYellow1). The fluorescent proteins are predominantly expressed in the cytoplasm, allowing the cells to be labeled for cell-tracing and cell-tracking studies.

For fluorescent labeling of specific cellular organelles or structures, visit the fluorescent subcellular labeling lentivirus page.

Cat. # Product Size Price License Quantity Details
0039VCT rLV.EF1.AmCyan1-9 1 x 20 uL USD $650.00

License Statement

ID Number  
261 Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
268 cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

The Vectalys Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like
0011VCT rLV.EF1.AmCyan1-9 3 x 20 uL USD $1798.00

License Statement

ID Number  
261 Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
268 cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

The Vectalys Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

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Tracking of mammalian cells transduced with lentivirus expressing AmCyan1 fluorescent protein (Cat. # 0011VCT rLV.EF1.AmCyan1-9)

Tracking of mammalian cells transduced with lentivirus expressing AmCyan1 fluorescent protein (Cat. # 0011VCT rLV.EF1.AmCyan1-9)

Tracking of mammalian cells transduced with lentivirus expressing AmCyan1 fluorescent protein (Cat. # 0011VCT rLV.EF1.AmCyan1-9).

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Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins.

0037VCT rLV.EF1.mCherry-9 1 x 20 uL USD $650.00

License Statement

ID Number  
261 Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511; 7,157,566; 7,393,923 and 7,250,298.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the rLV.EF1.mCherry-9 product License Agreement can be found by clicking here.
268 cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).
*

The Vectalys Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like
0009VCT rLV.EF1.mCherry-9 3 x 20 uL USD $1798.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511; 7,157,566; 7,393,923 and 7,250,298.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the rLV.EF1.mCherry-9 product License Agreement can be found by clicking here.
261 Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
268 cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).
*

The Vectalys Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

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Various cell types can be transduced with Vectalys lentiviral particles, including primary and stem cell lines

Various cell types can be transduced with Vectalys lentiviral particles, including primary and stem cell lines
Various cell types can be transduced with Vectalys lentiviral particles, including primary and stem cell lines. The suggested seeding concentration and MOI vary depending on the specific cell type to be transduced. When the recommendations in this table are followed, transduction efficiency is high for immortalized, primary, and stem cells, regardless of whether they are adherent or suspension cells.

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Overview of the process for generating high-titer lentiviral particles

Overview of the process for generating high-titer lentiviral particles
Overview of the process for generating high-titer lentiviral particles. Through this proprietary, large-scale process Vectalys is able to produce very high-titer, high-purity lentiviruses ready for transduction of many different cell types. The high titer allows for manipulation of even hard-to-transduce cells, like primary cells or stem cells, and the exceptional purity prevents cytotoxicity.

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Comparison of the purity of lentiviruses generated with Vectalys’ method or a classic purification method

Comparison of the purity of lentiviruses generated with Vectalys’ method or a classic purification method
Comparison of the purity of lentiviruses generated with Vectalys’ method or a classic purification method. Lentiviral proteins were separated by SDS-PAGE and stained with Coomassie Blue. The high purity of Vectalys lentiviral particles is clear when comparing the two methods; even when Vectalys particles are loaded at a 10-fold higher amount, very little contaminating protein is seen. The ratio of total particles (physical particles) to functional particles (transducing units) is lower for Vectalys purified lentivirus compared to classic methods of purification, indicating higher purity. Data provided by Vectalys.

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Not all titers are the same! Comparison of cells transduced with 100 M

Not all titers are the same! Comparison of cells transduced with 100 M

Not all titers are the same! Comparison of cells transduced with 100 M.O.I. of lentivirus provided by Clontech/Vectalys versus 100 M.O.I. of lentivirus provided by Vendor S. Each premade virus preparation expresses a green fluorescent protein when transduced. Clontech/Vectalys provides a functional titer, so every cell treated with our virus preparation was transduced with multiple copies of virus, leading to strong expression of the fluorescent protein. By contrast, the titer provided by Vendor S includes nonfunctional lentivirus particles as a large percentage of the titer so 100 M.O.I of their virus preparation is not sufficient to effectively transduce the cells.

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What is a functional titer? Most vendors of ready-made viral particles determine their titers using a simple particle count

What is a functional titer? Most vendors of ready-made viral particles determine their titers using a simple particle count

What is a functional titer? Most vendors of ready-made viral particles determine their titers using a simple particle count. This count will include free capsid protein, nonfunctional and immature virions, as well as functional virus.The functional titer provided by Clontech and Vectalys is not just the total particle number, it is a measurement of the total number of virions capable of integrating into cells, measured by transducing HCT116 cells. In real terms, this means our viral preps contain up to 100-fold more infectious particles than preps from other vendors.

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Achieve 100% transduction efficiency even in cell types that are typically hard to infect

Achieve 100% transduction efficiency even in cell types that are typically hard to infect
Achieve 100% transduction efficiency even in cell types that are typically hard to infect. Super-concentrated highly purified lentivirus prepared by Vectalys permits transduction at high M.O.I.
0036VCT rLV.EF1.tdTomato-9 1 x 20 uL USD $650.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511; 7,157,566; 7,393,923 and 7,250,298.
261 Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
268 cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the rLV.EF1.tdTomato-9 product License Agreement can be found by clicking here.
*

The Vectalys Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like
0008VCT rLV.EF1.tdTomato-9 3 x 20 uL USD $1798.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511; 7,157,566; 7,393,923 and 7,250,298.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the rLV.EF1.tdTomato-9 product License Agreement can be found by clicking here.
261 Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
268 cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).
*

The Vectalys Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins.

0038VCT rLV.EF1.ZsGreen1-9 1 x 20 uL USD $650.00

License Statement

ID Number  
261 Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
268 cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

The Vectalys Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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0010VCT rLV.EF1.ZsGreen1-9 3 x 20 uL USD $1798.00

License Statement

ID Number  
261 Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
268 cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

The Vectalys Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Whole-cell labeling using Clontech's ZsGreen1 fluorescent protein delivered using premade lentivirus (Cat. # 0010VCT rLV.EF1.ZsGreen1-9); nucleus is stained using DAPI. ZsGreen1 is 2.5X brighter than EGFP

Whole-cell labeling using Clontech's ZsGreen1 fluorescent protein delivered using premade lentivirus (Cat. # 0010VCT rLV.EF1.ZsGreen1-9); nucleus is stained using DAPI. ZsGreen1 is 2.5X brighter than EGFP

Whole-cell labeling using Clontech's ZsGreen1 fluorescent protein delivered using premade lentivirus (Cat. # 0010VCT rLV.EF1.ZsGreen1-9); nucleus is stained using DAPI. ZsGreen1 is 2.5X brighter than EGFP.

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Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins.

0040VCT rLV.EF1.ZsYellow1-9 1 x 20 uL USD $650.00

License Statement

ID Number  
261 Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
268 cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).
24 The RCFPs (including DsRedExpress, DsRedExpress2, and E2-Crimson) are covered by one or more of the following U.S. Patent Nos. 7,166,444; 7,157,565; 7,217,789; 7,150,979; 8,093,450 and 8,461,769.

The Vectalys Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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0012VCT rLV.EF1.ZsYellow1-9 3 x 20 uL USD $1798.00

License Statement

ID Number  
24 The RCFPs (including DsRedExpress, DsRedExpress2, and E2-Crimson) are covered by one or more of the following U.S. Patent Nos. 7,166,444; 7,157,565; 7,217,789; 7,150,979; 8,093,450 and 8,461,769.
261 Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
268 cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

The Vectalys Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

Mammalian cells transduced with lentivirus (Cat. # 0012VCT rLV.EF1.ZsYellow1-9) expressing ZsYellow1 fluorescent protein

Mammalian cells transduced with lentivirus (Cat. # 0012VCT rLV.EF1.ZsYellow1-9) expressing ZsYellow1 fluorescent protein

Mammalian cells transduced with lentivirus (Cat. # 0012VCT rLV.EF1.ZsYellow1-9) expressing ZsYellow1 fluorescent protein.

Back

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins.

*You must be logged in to a Business Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Tips and FAQs Lentiviral particle FAQs
Overviews Premade lentiviral particles
Watch our webinar to learn about highly pure, highly concentrated premade lentivirus Premade lentivirus webinar

Overview

  • What does 109 TU/ml mean?
    • In each microliter, there will be at least 1 x 106 functional lentivirus particles.
    • Each 60-µl kit is sufficient for 30 transductions (using an MOI of 10) in a 6-well plate containing 2 x 105 cells/well.
  • Premium quality—produced using a multi-step purification process that results in:
    • Very high titer; functional titer of >1 x 109 transduction units per ml (TU/ml).
    • High purity—transduce target cells without cytotoxicity.
    • >90% transduction efficiency even at very low MOI.
  • Functional titer provided, not a just a particle count
  • tdTomato and ZsGreen1 are exceptionally bright fluorescent proteins, 6X and 2.5X brighter than EGFP, respectively.
    • Learn more about our fluorescent protein portfolio.
  • These vectors have a puromycin resistance cassette and the stable clones integrating these constructs can be selected using puromycin.

More Information

Applications

  • Labeling and tracking mammalian cells using bright fluorescent proteins

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Learn more about the technology used to make our ready-to-use lentivirus particle preps

Super-pure and concentrated premade lentivirus particles

Unlike ready-to-use lentiviral particles from other suppliers, the particles in our catalog are produced via a multistep purification process that results in very high titers and high purity. High titers increase transduction efficiency, while high purity reduces cytotoxicity, allowing you to successfully transduce a large number of different cell types—including primary, immortalized, adherent, and suspension cells—while retaining high cell viability.

Technology overview Lentiviral particles FAQs

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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