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Lentivirus

Lentiviral particles for fluorescent whole-cell labeling

Cells transduced with these premade, super-high titer particles will express a bright fluorescent protein in one of four colors: red (TdTomato or mCherry), green (ZsGreen1), cyan (AmCyan1), or yellow (ZsYellow1). The fluorescent proteins are predominantly expressed in the cytoplasm, allowing the cells to be labeled for cell-tracing and cell-tracking studies.

For fluorescent labeling of specific cellular organelles or structures, visit the fluorescent subcellular labeling lentivirus page.

Cat. #

Product

Size

License

Quantity

Details

0040VCT

rLV.EF1.ZsYellow1-9

1 x 20 uL

USD $714.00

License Statement

ID Number

261

Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com

268

cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

The Flash Therapeutics Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

0039VCT

rLV.EF1.AmCyan1-9

1 x 20 uL

USD $714.00

License Statement

ID Number

261

268

Notice to purchaser

Components

0038VCT

rLV.EF1.ZsGreen1-9

1 x 20 uL

USD $714.00

License Statement

ID Number

261

268

Notice to purchaser

Components

0037VCT

rLV.EF1.mCherry-9

1 x 20 uL

USD $714.00

License Statement

ID Number
261	Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511 and 7,393,923.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the rLV.EF1.mCherry-9 product License Agreement can be found by clicking here.
268	cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

Notice to purchaser

Components

0036VCT

rLV.EF1.tdTomato-9

1 x 20 uL

USD $714.00

License Statement

ID Number
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511 and 7,393,923.
261	Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
268	cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the rLV.EF1.tdTomato-9 product License Agreement can be found by clicking here.

Notice to purchaser

Components

0012VCT

rLV.EF1.ZsYellow1-9

3 x 20 uL

USD $1938.00

License Statement

ID Number

261

268

Notice to purchaser

Components

0011VCT

rLV.EF1.AmCyan1-9

3 x 20 uL

USD $1938.00

License Statement

ID Number

261

268

Notice to purchaser

Components

0010VCT

rLV.EF1.ZsGreen1-9

3 x 20 uL

USD $1938.00

License Statement

ID Number

261

268

Notice to purchaser

Components

0009VCT

rLV.EF1.mCherry-9

3 x 20 uL

USD $1938.00

License Statement

ID Number
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511 and 7,393,923.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the rLV.EF1.mCherry-9 product License Agreement can be found by clicking here.
261	Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
268	cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

Notice to purchaser

Components

0008VCT

rLV.EF1.tdTomato-9

3 x 20 uL

USD $1938.00

License Statement

ID Number
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511 and 7,393,923.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the rLV.EF1.tdTomato-9 product License Agreement can be found by clicking here.
261	Vectalys’ technology Vectalys’ highly concentrated and purified lentiviral vectors and its use are covered by U.S. Patent No. 13/0029379 and WO 2013/014537. The purchase price of this product includes limited, nontransferable rights to use this product solely for internal research purposes. Academic and not-for-profit research institutions whose research using the Vectalys’ Technology or the product obtained by the Vectalys’ technology is sponsored by for profit organizations, which shall receive ownership to all data and results stemming from the sponsored research, shall need a commercial license agreement from Vectalys in order to use the Vectalys’ Technology or the product obtained by the Vectalys’ technology. Please contact Vectalys for information on purchasing a license to use these products for commercial purposes: Parc Technologique du Canal, Bâtiment Canal Biotech II, 3, rue des satellites, 31400 Toulouse (France), TEL: +33(0)5 61 28 70 75, EMAIL: sandy.darrigan@vectalys.com
268	cPPT Element cPPT Element and its use are covered by one or more patents: FR9805197 and US 60/158387 and/or other pending foreign patent applications licensed to Vectalys. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Vectalys is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

Notice to purchaser

Components

Overview

What does 10⁹ TU/ml mean?
- In each microliter, there will be at least 1 x 10⁶ functional lentivirus particles.
- Each 60-µl kit is sufficient for 30 transductions (using an MOI of 10) in a 6-well plate containing 2 x 10⁵ cells/well.
Premium quality—produced using a multi-step purification process that results in:
- Very high titer; functional titer of >1 x 10⁹ transduction units per ml (TU/ml).
- High purity—transduce target cells without cytotoxicity.
- >90% transduction efficiency even at very low MOI.
Functional titer provided, not a just a particle count
tdTomato and ZsGreen1 are exceptionally bright fluorescent proteins, 6X and 2.5X brighter than EGFP, respectively.
- Learn more about our fluorescent protein portfolio.
These vectors have a puromycin resistance cassette and the stable clones integrating these constructs can be selected using puromycin.

More Information

Applications

Labeling and tracking mammalian cells using bright fluorescent proteins

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Learn more about the technology used to make our ready-to-use lentivirus particle preps

Super-pure and concentrated premade lentivirus particles

Unlike ready-to-use lentiviral particles from other suppliers, the particles in our catalog are produced via a multistep purification process that results in very high titers and high purity. High titers increase transduction efficiency, while high purity reduces cytotoxicity, allowing you to successfully transduce a large number of different cell types—including primary, immortalized, adherent, and suspension cells—while retaining high cell viability.

Technology overview Lentiviral particles FAQs

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Lentiviral particles for fluorescent whole-cell labeling

Notice to purchaser

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

0040VCT: rLV.EF1.ZsYellow1-9

Notice to purchaser

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

0039VCT: rLV.EF1.AmCyan1-9

Notice to purchaser

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

0038VCT: rLV.EF1.ZsGreen1-9

Notice to purchaser

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

0037VCT: rLV.EF1.mCherry-9

Notice to purchaser

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

0036VCT: rLV.EF1.tdTomato-9

Notice to purchaser

Mammalian cells transduced with lentivirus (Cat. # 0012VCT rLV.EF1.ZsYellow1-9) expressing ZsYellow1 fluorescent protein

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

0012VCT: rLV.EF1.ZsYellow1-9

Notice to purchaser

Tracking of mammalian cells transduced with lentivirus expressing AmCyan1 fluorescent protein (Cat. # 0011VCT rLV.EF1.AmCyan1-9)

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

0011VCT: rLV.EF1.AmCyan1-9

Notice to purchaser

Whole-cell labeling using Clontech's ZsGreen1 fluorescent protein delivered using premade lentivirus (Cat. # 0010VCT rLV.EF1.ZsGreen1-9); nucleus is stained using DAPI. ZsGreen1 is 2.5X brighter than EGFP

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

0010VCT: rLV.EF1.ZsGreen1-9

Notice to purchaser

Various cell types can be transduced with Vectalys lentiviral particles, including primary and stem cell lines

Overview of the process for generating high-titer lentiviral particles

Comparison of the purity of lentiviruses generated with Vectalys’ method or a classic purification method

Not all titers are the same! Comparison of cells transduced with 100 M

What is a functional titer? Most vendors of ready-made viral particles determine their titers using a simple particle count

Achieve 100% transduction efficiency even in cell types that are typically hard to infect

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

0009VCT: rLV.EF1.mCherry-9

Notice to purchaser

Map schematics of expression cassettes contained in particles for whole-cell labeling with fluorescent proteins

0008VCT: rLV.EF1.tdTomato-9

Overview

More Information

Applications

Additional product information