Lentivirus titration by p24 ELISA
The Lenti-X p24 Rapid Titer Kit (Single Wash) and the Lenti-X p24 Rapid Titer Kit are designed for the rapid determination of lentiviral supernatant titers by p24 ELISA. (See the workflow comparison for both the kits.)
Why do you need to know your titer?
- Confirm the success of your packaging reaction to avoid wasting time with your experiments
- Perform consistent experiments
- Ensure sufficient MOI so that 100% of your cells are transduced
- Control copy number and hence expression levels
The Lenti-X p24 Rapid Titer Kit (Single Wash) and the Lenti-X p24 Rapid Titer Kit are designed for the rapid determination of lentiviral supernatant titers by p24 ELISA.
Why do you need to know your titer?
- Confirm the success of your packaging reaction to avoid wasting time with your experiments
- Perform consistent experiments
- Ensure sufficient MOI so that 100% of your cells are transduced
- Control copy number and hence expression levels
What is p24?
Recombinant lentiviruses generated for research use contain major structural proteins, including envelope proteins, matrix proteins, and virus core proteins. The gag gene encodes the viral capsid protein (p24), the nucleocapsid proteins (p6 and p7), and a matrix protein (p17) (see data images by expanding the Details tab in the product table). The Lenti-X p24 Rapid Titer Kit specifically measures the amount of p24 capsid protein present in your viral supernatant. The level of p24 correlates directly with virus titer.
Overview
Lenti-X p24 Rapid Titer Kit (Single Wash)
- Fast and easy-to-use p24 ELISA kit to determine the titer of your lentivirus prep (~1.5 hr)
- Broader detection range enables determination of p24 titer with fewer dilution steps and iterations
- Collect lentiviral supernatant, lyse, bind, wash (1X), and detect
- 96-well format in separable 12 x 8-well strips, offering flexibility
- p24 control included
Lenti-X p24 Rapid Titer Kit
- Rapidly determine the titer of your lentivirus prep using a p24 ELISA (~3 hr)
- Collect lentiviral supernatant, lyse, bind, wash (3X), and detect
- 96-well format in separable 12 x 8-well strips offering flexibility
- p24 control included
More Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Lentivirus titration kit options
We have several options available for determining the titer of your lentiviral preps. From the quick and easy Lenti-X GoStix Plus to the precise Lenti-X qRT-PCR Titration Kit, we have the solution you need to quantify lentiviral titers.
Kit options Virus product finder toolTakara Bio USA, Inc.
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