Integrated lentivirus copy number quantification

Our newest product, the LVpro Provirus Quantitation Kit, measures the number of vector copies (provirus copy number) incorporated into the genome when genes are transferred into human cells by lentiviral vectors using real-time PCR. This kit uses two probes—one for the internal control and another for the provirus copy—providing more accurate measurement of the proviral copies compared to our previous kit, Lenti-X Provirus Quantitation Kit.

Determine precise effective lentivirus titers

By quantifying the number of lentiviruses that have integrated into the nuclear DNA of your target cells, you can precisely determine the real titer (i.e., effective titer) of your lentiviral supernatant stock and use this information to infect cells with greater precision and a more highly defined MOI (multiplicity of infection), allowing you to predict how many viral genomes will integrate into your cells. You can also use this method to calibrate other titration methods that measure virus content in packaging-cell supernatants, but do not measure functional integrative titers in your target cells.