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Lentivirus

Lenti-X Transduction Sponge

The Lenti-X Transduction Sponge is a novel technology that allows you to achieve microfluidics-driven lentiviral transduction efficiency gains without the use of microfluidics-based instrumentation. With one sponge, you can transduce 10⁵–10⁷ cells without the need for time-consuming spinoculation or harsh, chemical transduction enhancers that require optimization to retain cell viability. The Lenti-X Transduction Sponge is effective in transducing a wide variety of cell types, including cell lines and difficult-to-transduce primary cells.

How does the Lenti-X Transduction Sponge work?

The Lenti-X Transduction Sponge is a novel technology that allows you to achieve microfluidics-driven lentiviral transduction efficiency gains without the use of microfluidics-based instrumentation. With one sponge, you can transduce 10⁵–10⁷ cells without the need for time-consuming spinoculation or harsh chemical transduction enhancers that require optimization to retain cell viability. The Lenti-X Transduction Sponge enables you to effectively transduce a wide variety of cell types, including cell lines and difficult-to-transduce primary cells.

How does the Lenti-X Transduction Sponge work?

A mixture of target cells and lentivirus is added onto the sponge and incubated for 1 hr at 37°C to allow the mixture to be absorbed. Following absorption, cell culture media is added to the sponge and then incubated at 37°C for 16–24 hr.

The following day, you can easily release the transduced cells by dissolving the sponge in a special release buffer. These cells can then be replated for further expansion and downstream applications. The user-friendly protocol minimizes cell handling and requires smaller total reaction volumes, reducing the amount of virus needed. The Lenti-X Transduction Sponge consistently delivers transduction efficiencies comparable to or superior to traditional methods.

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Cat. #

Product

Size

Price

License

Quantity

Details

631478

Lenti-X™ Transduction Sponge

24 Rxns

USD $520.00

License Statement

ID Number

446

Limited Use Label License: Lenti-X Transduction Sponge

This product which you will receive/have received from Takara Bio USA, Inc. (“TBUSA”) includes within it certain materials (“Product”) which are subject to a field limited license between TBUSA and North Carolina State University (“NC State”) and are subject to one or more pending patents including U.S. Patent Application No. 17/917,770 and corresponding foreign patent filings.

As the purchaser of the Product (“Recipient”), Recipient hereby agrees to the following limited use license: Recipient is granted the limited right to use the Product only for ex vivo transduction of cells for internal non-commercial research purposes. The Recipient is strictly prohibited from: (i) transferring the Product its components, and/or materials that incorporate the Product to any third party without TBUSA’s express written permission; (ii) using the ex vivo transduced cells and/or any components thereof in any clinical or therapeutic application without TBUSA’s express written permission; (iii) using the Product for any application other than the ex vivo transduction of cells without NC State’s express written permission; and/or (iv) implanting/inserting the Product within a living organism without NC State’s express written permission.

The Recipient acknowledges and hereby agrees that: (i) if Recipient is interested in implanting/inserting the Product within a living organism and/or in using the Product for applications other than ex vivo transduction of cells, Recipient must obtain a license from NC State, and (ii) NC State is a third-party beneficiary under this limited use label license and has the authority to enforce its provision against the Recipient, at its sole discretion, if the Recipient (a) uses the Product for any application other than the ex vivo transduction of cells, and/or (b) implants/inserts the Product in a living organism. Any use of the Product other than what is expressly granted above, will require an additional license from TBUSA. Any use of the Product for in vivo transduction of cells and/or for implantation/insertion within a living organism, will require a license from NC State. For information relating to obtaining a license from NC State, contact NC State at ncsulicenses@ncsu.edu.

The Lenti-X Transduction Sponge is a macroporous alginate structure that facilitates rapid and efficient lentiviral transduction without spinoculation or the use of a chemical transduction enhancer. It encourages the co-localization of target cells and lentivirus, eliminating biotransport issues associated with standard lentiviral transduction methods. A simple, easy-to-use protocol reduces cell handling and requires smaller total reaction volumes while producing transduction efficiencies that are comparable to or improved over traditional methods.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

Overview

A better alternative to standard lentiviral transduction methods.

Achieve microfluidics-driven lentiviral transduction efficiency gains without the use of microfluidics-based instruments
Skip labor-intensive, time-consuming spinoculation that requires additional centrifugation equipment
Transduce 10⁵–10⁷ cells in the volume required for only one well of a 24-well plate with each sponge
Use the same easy protocol on a wide variety of cell types (including cell lines and primary cells)
End reliance on chemical-based enhancers (e.g. polybrene) that are toxic to certain cell types and require optimization to maintain cell viability during and after transduction.

Easy-to-use workflow that reduces cell handling

More Information

Applications

Lentiviral transduction for the following applications:
- Gene expression
- Protein expression
- Cell line generation
- Therapeutic model generation
- Whole-genome screening
- CAR-T research using lentivirus for gene modification
Transduction of retrovirus and virus-like particles (VLPs)

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Lenti-X Transduction Sponge: A novel technology for lentiviral transduction

Learn how to use this macroporous alginate structure to facilitate rapid and efficient lentiviral transduction without spinoculation or the use of chemical transduction enhancers.

Sample the Lenti-X Transduction Sponge

Evaluate the transduction sponge in your research project.

Skip labor-intensive, time-consuming spinoculation
End reliance on chemical-based enhancers like polybrene
Transduce 10⁵–10⁷ cells in one reaction

Request a sample

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2025 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Lenti-X Transduction Sponge

How does the Lenti-X Transduction Sponge work?

How does the Lenti-X Transduction Sponge work?

Notice to purchaser

Lenti-X Transduction Sponge workflow

Convenient format for use with a 24-well tissue culture plate

Transduction efficiency compared to spinoculation

Increased T-cell transduction efficiency while preserving viability

Consistent transduction efficiency across varying cell amounts and transduction times with low inter-sponge variability

631478: Lenti-X Transduction Sponge

Overview

A better alternative to standard lentiviral transduction methods.

Easy-to-use workflow that reduces cell handling

More Information

Applications

Additional product information

Evaluate the transduction sponge in your research project.