Lentivirus titration by qRT-PCR
The Lenti-X qRT-PCR Titration Kit provides a fast and simple method for measuring lentivirus titer. The kit uses a quick RNA purification step before quantifying the number of lentiviral genome copies using real-time PCR.
The Lenti-X qRT-PCR Titration Kit provides a fast and simple method for measuring lentivirus titer. The kits use a quick RNA purification step before quantifying the number of lentiviral genome copies using real-time PCR.
Rapid determination of lentiviral titer
Whereas traditional titration methods require up to 10 days to complete, these titration kits require only 4 hours and work with all HIV-1-based lentiviral vectors. Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell infection, allowing you to perform both on the same day.
Achieve consistent lentiviral transductions
A major advantage of lentiviral gene-delivery systems is the ability to control the level of gene expression by adjusting the multiplicity of infection (MOI) to dictate the number of viral genomes that enter your target cells. These titration kits allow you to make a rapid correlation between the lentiviral copy number and lentiviral infectivity in your cell type and ultimately allow you to control the expression level between experiments.
How it works
The qRT-PCR procedure entails subjecting serial dilutions of your sample and a control RNA of known copy number (provided) to one-step qRT-PCR. The lentiviral copy number contained in your sample can then be determined by comparing its Ct value to the standard curve.
RNA purification and DNase I treatment steps are critical to the protocol so even viruses packaged using transient transfections—which always contain residual plasmid DNA—can be accurately quantified using this kit.
The kit includes sufficient material for 200 qRT-PCR reactions.
- Confirm the success of your lentiviral packaging reaction
- Perform consistent experiments
- Ensure sufficient MOIs so that 100% of your cells are transduced
- Control copy number and hence expression levels
- 200 qRT-PCR reactions are included*
- Purify 10 different virus preps
*The primers included in this kit are designed to amplify a conserved region of HIV-1 genome adjacent to the packaging signal. These primers are compatible with all lentiviral vectors sold by Takara Bio and most commonly used lentiviral vectors. If you are unsure whether this kit is compatible with your vector, please send your vector sequence to Technical_Support@takarabio.com, and our support team will be happy to confirm primer compatibility.
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Lentivirus titration kit options
We have several options available for determining the titer of your lentiviral preps. From the quick and easy Lenti-X GoStix Plus to the precise Lenti-X qRT-PCR Titration Kit, we have the solution you need to quantify lentiviral titers.Kit options Virus product finder tool
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