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  • Lenti-X Transduction Sponge
  • Lenti-X T-Cell Transduction Sponge
Home › Products › Gene function › Viral transduction › Lentivirus › Transduction sponges › Lenti-X T-Cell Transduction Sponge

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Lenti-X T-Cell Transduction Sponge

The Lenti‑X T‑Cell Transduction Sponge is a novel technology that allows you to achieve microfluidics-driven lentiviral transduction efficiency gains in T cells without the use of microfluidics-based instrumentation. One sponge infused with an optimized blend of T-cell activation reagents (rhIL-2 and anti-human CD3 and CD28 antibodies) enables T‑cell activation and transduction in a single step of 105–107 cells without the need for time-consuming spinoculation or harsh, chemical transduction enhancers that require optimization to retain cell viability.

How does the Lenti‑X T‑Cell Transduction Sponge work?

The Lenti‑X T‑Cell Transduction Sponge is a novel technology that allows you to achieve microfluidics-driven lentiviral transduction efficiency gains in T cells without the use of microfluidics-based instrumentation. One sponge infused with an optimized blend of T-cell activation reagents (rhIL-2 and anti-human CD3 and CD28 antibodies) enables T‑cell activation and transduction in a single step of 105–107 cells without the need for time-consuming spinoculation or harsh, chemical transduction enhancers that require optimization to retain cell viability.

How does the Lenti‑X T‑Cell Transduction Sponge work?

A mixture of target cells (T cells or PBMCs) and lentivirus are added onto the sponge and incubated for 1 hr at 37°C to allow the mixture to be absorbed. Following absorption, cell culture media is added to the sponge, which is then incubated at 37°C for 48 hr.

After the incubation, you can easily release the transduced cells by infusing the sponge with a special release buffer. The sponge will dissolve during the subsequent wash steps, but this does not affect cell viability. These cells can then be plated for further expansion and downstream applications. The user-friendly protocol minimizes cell handling and requires smaller total reaction volumes, reducing the amount of virus needed. The Lenti‑X T‑Cell Transduction Sponge consistently delivers transduction efficiencies comparable to or superior to traditional methods.

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Cat. # Product Size Price License Quantity Details
631480 Lenti-X™ T-Cell Transduction Sponge 12 Rxns USD $624.00

License Statement

ID Number  
446 Limited Use Label License: Lenti-X Transduction Sponge

This product which you will receive/have received from Takara Bio USA, Inc. (“TBUSA”) includes within it certain materials (“Product”) which are subject to a field limited license between TBUSA and North Carolina State University (“NC State”) and are subject to one or more pending patents including U.S. Patent Application No. 17/917,770 and corresponding foreign patent filings.

As the purchaser of the Product (“Recipient”), Recipient hereby agrees to the following limited use license: Recipient is granted the limited right to use the Product only for ex vivo transduction of cells for internal non-commercial research purposes. The Recipient is strictly prohibited from: (i) transferring the Product its components, and/or materials that incorporate the Product to any third party without TBUSA’s express written permission; (ii) using the ex vivo transduced cells and/or any components thereof in any clinical or therapeutic application without TBUSA’s express written permission; (iii) using the Product for any application other than the ex vivo transduction of cells without NC State’s express written permission; and/or (iv) implanting/inserting the Product within a living organism without NC State’s express written permission.

The Recipient acknowledges and hereby agrees that: (i) if Recipient is interested in implanting/inserting the Product within a living organism and/or in using the Product for applications other than ex vivo transduction of cells, Recipient must obtain a license from NC State, and (ii) NC State is a third-party beneficiary under this limited use label license and has the authority to enforce its provision against the Recipient, at its sole discretion, if the Recipient (a) uses the Product for any application other than the ex vivo transduction of cells, and/or (b) implants/inserts the Product in a living organism. Any use of the Product other than what is expressly granted above, will require an additional license from TBUSA. Any use of the Product for in vivo transduction of cells and/or for implantation/insertion within a living organism, will require a license from NC State. For information relating to obtaining a license from NC State, contact NC State at ncsulicenses@ncsu.edu.

The Lenti-X T-Cell Transduction Sponge expedites and streamlines lentiviral transduction of T cells, bypassing the need for prior activation or spinoculation with chemical enhancers. This innovative approach employs a macroporous alginate matrix infused with an optimized blend of rhIL-2 (100 IU) and anti-human CD3 & CD28 antibodies. This combination with the sponge not only activates T cells but also enhances lentiviral transduction by fostering the co-localization of activated T cells and lentivirus particles.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Lenti-X T-Cell Transduction Sponge details

Lenti-X T-Cell Transduction Sponge details

Lenti-X T-Cell Transduction Sponge. Panel A. Sponges (12) in the crush-resistant blister packaging and magnified view of the macroporous cell structure of the alginate. Panel B. Illustrated visualization of colocalization of T cells and virus promoted by the sponge structure.

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Lenti-X T-Cell Transduction Sponge workflow

Lenti-X T-Cell Transduction Sponge workflow

Visual overview of the Lenti-X T-Cell Transduction Sponge workflow.

Overview

  • Conduct T‑cell activation and transduction in one step
  • Achieve microfluidics-driven lentiviral transduction efficiency gains without the use of microfluidics-based instruments
  • Skip labor-intensive, time-consuming spinoculation that requires additional centrifugation equipment
  • Transduce 105–107 cells in the volume required for only one well of a 24‑well plate with each sponge
  • End reliance on chemical-based enhancers (e.g., polybrene) that are toxic to certain cell types and require optimization to maintain cell viability during and after transduction.

More Information

Easy‑to‑use workflow that reduces cell handling

Applications

  • Lentiviral transduction for the following applications:
    • T‑cell engineering
    • Gene editing
    • Gene expression
    • Protein expression
    • Cell line generation
    • Therapeutic model generation
    • Whole-genome screening
    • CAR‑T research using lentivirus for gene modification
  • Transduction of retrovirus and virus-like particles (VLPs)

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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  • RNA-seq
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  • Signature enzymes
  • High-throughput real-time PCR solutions
  • Detection assays
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  • Media, differentiation kits, and matrices
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  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
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