Lenti-X T-Cell Transduction Sponge
The Lenti‑X T‑Cell Transduction Sponge is a novel technology that allows you to achieve microfluidics-driven lentiviral transduction efficiency gains in T cells without the use of microfluidics-based instrumentation. One sponge infused with an optimized blend of T-cell activation reagents (rhIL-2 and anti-human CD3 and CD28 antibodies) enables T‑cell activation and transduction in a single step of 2 x 106–107 cells without the need for time-consuming spinoculation or harsh, chemical transduction enhancers that require optimization to retain cell viability.
How does the Lenti‑X T‑Cell Transduction Sponge work?
The Lenti‑X T‑Cell Transduction Sponge is a novel technology that allows you to achieve microfluidics-driven lentiviral transduction efficiency gains in T cells without the use of microfluidics-based instrumentation. One sponge infused with an optimized blend of T-cell activation reagents (rhIL-2 and anti-human CD3 and CD28 antibodies) enables T‑cell activation and transduction in a single step of 2 x 106–107 cells without the need for time-consuming spinoculation or harsh, chemical transduction enhancers that require optimization to retain cell viability.
How does the Lenti‑X T‑Cell Transduction Sponge work?
A mixture of target cells (T cells or PBMCs) and lentivirus are added onto the sponge and incubated for 1 hr at 37°C to allow the mixture to be absorbed. Following absorption, cell culture media is added to the sponge, which is then incubated at 37°C for 48 hr.
After the incubation, you can easily release the transduced cells by infusing the sponge with a special release buffer. The sponge will dissolve during the subsequent wash steps, but this does not affect cell viability. These cells can then be plated for further expansion and downstream applications. The user-friendly protocol minimizes cell handling and requires smaller total reaction volumes, reducing the amount of virus needed. The Lenti‑X T‑Cell Transduction Sponge consistently delivers transduction efficiencies comparable to or superior to traditional methods.
Overview
- Conduct T‑cell activation and transduction in one step
- Achieve microfluidics-driven lentiviral transduction efficiency gains without the use of microfluidics-based instruments
- Skip labor-intensive, time-consuming spinoculation that requires additional centrifugation equipment
- Transduce 2 x 106–107 cells in the volume required for only one well of a 24‑well plate with each sponge
- End reliance on chemical-based enhancers (e.g., polybrene) that are toxic to certain cell types and require optimization to maintain cell viability during and after transduction.
More Information
Easy‑to‑use workflow that reduces cell handling
Applications
- Lentiviral transduction for the following applications:
- T‑cell engineering
- Gene editing
- Gene expression
- Protein expression
- Cell line generation
- Therapeutic model generation
- Whole-genome screening
- CAR‑T research using lentivirus for gene modification
- Transduction of retrovirus and virus-like particles (VLPs)
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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