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Home › Products › Gene function › Viral transduction › Lentivirus › Premade lentiviral particles › Tet-On 3G transactivator

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Tet-On 3G transactivator lentiviral particles

Premade lentivirus to express the Tet-On 3G transactivator

Highly concentrated and purified lentivirus for delivery of the Tet-On 3G transactivator to target cells.  Any gene of interest (GOI) under the control of a TRE3G promoter (PTRE3G) can be tightly controlled by the presence and absence of doxycycline (Dox) in the culture medium. Learn more about Tet-On 3G inducible gene expression.

Cat. # Product Size Price License Quantity Details
0055VCT rLV.EF1.Tet3G-9 1 x 20 uL $625.00 *

Highly concentrated and purified lentivirus for delivery of the Tet-On 3G transactivator to target cells.  Any gene of interest under the control of a TRE3G promoter can be tightly controlled by the presence and absence of Dox in the culture medium. Tet-On 3G is a modified, improved Tet-On Advanced transcriptional activator protein (transactivator) which has been evolved to display far higher sensitivity to the inducer molecule dox. Tet-On 3G generates very high maximal expression and responds to lower Dox concentrations than its predecessors.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL $850.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data
0027VCT rLV.EF1.Tet3G-9 3 x 20 uL $1769.00 *

The Vectalys Inducible Reprogramming Lentiviral Vectors are prepackaged lentiviral particles expressing polycistronic Oct4, Sox2, KLF4, and Myc for reprogramming human somatic cells under the induction of doxycycline using Tet technology. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.). The expression of these four factors has been shown to reprogram a variety of differentiated cells into induced pluripotent stem (iPS) cells with embryonic stem (ES) cell-like properties.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL $850.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data

*You must be logged in to a Business Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Learn about premium premade lentivirus particles Premade lentiviral particles
FBS for Tet system Tet-approved FBS
Selection of tetracycline-inducible systems for your research Tet selection guide

Overview

  • What does 109 TU/ml mean?
    • In each microliter, there will be at least 1 x 106 functional lentivirus particles.
    • Each 60-µl kit is sufficient for 30 transductions (using an MOI of 10) in a 6-well plate containing 2 x 105 cells/well.
  • Gold standard tetracycline transactivator in a ready-to-use lentivirus format.
  • Premium quality; produced via a multistep purification process which results in very high titers and purity.
    • Functional titer provided, not a just a particle count.
    • High purity—transduce target cells without cytotoxicity.
    • >90% transduction efficiency can be obtained even at very low MOI.
  • Can be combined with any vector containing a TRE promoter to obtain tetracycline-inducible expression of your gene of interest (we recommend the TRE3G promoter).
  • Learn more about Tet-On 3G technology.

More Information

Applications

  • Tightly controlled, inducible expression of your gene of interest in hard-to-transfect mammalian cells

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Learn more about the technology used to make our ready-to-use lentivirus particle preps

Super-pure and concentrated premade lentivirus particles

Unlike ready-to-use lentiviral particles from other suppliers, the particles in our catalog are produced via a multistep purification process that results in very high titers and high purity. High titers increase transduction efficiency, while high purity reduces cytotoxicity, allowing you to successfully transduce a large number of different cell types—including primary, immortalized, adherent, and suspension cells—while retaining high cell viability.

Technology overview Lentiviral particles FAQs

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