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Easily deliver bright fluorescent proteins to your target cells
Ready-to-use lentiviral particles for fluorescent cell labeling
Labeling cells with fluorescent proteins—either for general cell marking or labeling specific subcellular structures—can be hindered by difficulties with gene delivery or an inability to obtain adequate fluorescent protein expression.
Our high-quality, ready-to-use lentiviral particles can be used to deliver and express ultra-bright fluorescent protein variants to virtually any human cell type—even those that are resistant to traditional methods of gene delivery (e.g., non-proliferating cells, stem cells, and terminally differentiated cells). These particles result in long-term, constitutive expression of untargeted, cytoskeleton-targeted, or organelle-targeted fluorescent proteins, generating cells that are suitable for:
- Investigating protein localization
- Monitoring protein trafficking
- Tracking cells in real time
- Studying organelle dynamics and morphology
Delivery of fluorescent proteins targeted to specific subcellular locations
(click to view larger size)
|Targeting protein/tag||Fluorescent protein options*|
|Actin||Human β-actin||AcGFP1, mCherry|
|Tubulin||Human α-tubulin||AcGFP1, mCherry|
*AcGFP1 and mCherry proteins are truly monomeric fluorescent proteins that are ideal fusion tags. AcGFP1 protein is very stable, allowing visualization over an extended period of time. mCherry protein is highly photostable and resistant to photobleaching. The distinct spectra of these fluorescent proteins allow them to be used in double-labeling experiments.
Delivery of untargeted fluorescent proteins
(click to view larger size)
|Excitation/emission maxima (nm)||Brightness (relative to GFP)|
*The majority of fluorescent protein will be present in the cytoplasm.
Takara Bio's Living Colors fluorescent proteins
Living Colors fluorescent proteins are detectable without added cofactors or substrates and can be detected directly by fluorescence microscopy. The fluorescent proteins (alone and fusions) do not compromise cell structure or interfere with cellular physiology, making them ideal tools for visualizing living cells.
Webinar: highly concentrated and pure lentiviral particle preps
Watch our webinar to learn all about genome editing with lentiviral particles. Topics covered include: how to produce high-quality and high-titer lentivirus, the differences between lentiviral production systems, and applications such as the use of lentivirus for tet-inducible gene expression or subcellular localization studies.Webinar Technology overview
Takara Bio USA, Inc.
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