mCherry fluorescent protein
mCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed. mCherry matures rapidly, making it possible to see results very soon after transfection or activation of transcription. It is highly photostable and resistant to photobleaching (Shaner et al. 2004). As a result, mCherry is now the most widely used and cited red fluorescent protein. mCherry is bright although tdTomato is the brightest commercially available red fluorescent protein.
Overview
- Bright red fluorescent protein
- Lentiviral (pLVX-) and retroviral (pRetroQ) formats are available
- Rapid maturation (t0.5=15 min)
- Monomeric structure
- Excitation maximum: 587 nm
Emission maximum: 610 nm - See also Photoactivatable mCherry (PAmCherry)
More Information
Applications
- Create an in-frame fusion to track your protein of interest (-N and -C vectors)
- Monitor transfection
- Promoter-reporter studies
- Quantitative imaging techniques
- Coexpress your protein and another protein of interest using an IRES
References
Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567–72 (2004).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Lentiviral fluorescent proteins
We offer a wide array of premade lentivirus particles for whole-cell labeling or for labeling subcellular compartments, such as mitochondria, golgi, membranes, etc.
Whole-cell labeling Subcellular-structure labelingTakara Bio USA, Inc.
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