ZsYellow1 fluorescent protein

ZsYellow1 has a true yellow emission, making it ideal for multicolor applications. Like all of our Living Colors fluorescent proteins, ZsYellow1 can be detected in cells without adding cofactors or substrates, which makes it ideal for use in live cell assays. The gene for ZsYellow1 has been human codon-optimized to enhance its translation in mammalian cells, and for higher solubility, brighter emission, and rapid chromophore maturation (8–12 hours).

The ZsYellow1 fluorescent protein can be used as a molecular tag or as a reporter to visualize, track, and quantify cellular processes including protein synthesis and turnover, protein translocation, gene induction, and cell lineage. Because its excitation and emission spectra are distinct from our other fluorescent proteins, ZsYellow1 can be used for multiplex applications—that is, to simultaneously detect two or more events in the same cell or cell population. For example, it can be used in two- and three-color analyses with AmCyan1 and/or HcRed1. Like all of our Living Colors fluorescent proteins, ZsYellow1 is ideal for monitoring gene expression and protein localization in vivo, in situ, and in real time.

We also offer a polyclonal anti-ZsYellow1 antibody.

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Cat. #	Product	Size
632445	pZsYellow1-N1 Vector	20 ug	USD $589.00
pZsYellow1-N1 is a human codon-optimized expression vector that encodes a variant of the Zoanthus sp. yellow fluorescent protein, ZsYellow1, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pZsYellow1-N1 allows genes cloned into the multiple cloning site (MCS) upstream of the ZsYellow1 coding sequence to be expressed as fusions to the N-terminus of the fluorescent protein. The unmodified vector will express ZsYellow1 in mammalian cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Schematic for fluorescent fusion protein vectors Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector. Back Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1 Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1. Back 632445: pZsYellow1-N1 Vector
632444	pZsYellow1-C1 Vector	20 ug	USD $589.00
pZsYellow1-C1 is a human codon-optimized expression vector that encodes a variant of the Zoanthus sp. yellow fluorescent protein, ZsYellow1, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pZsYellow1-C1 allows genes cloned into the multiple cloning site (MCS) downstream of the ZsYellow1 coding sequence to be expressed as fusions to the C-terminus of the fluorescent protein. The unmodified vector will express ZsYellow1 in mammalian cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Schematic for fluorescent fusion protein vectors Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector. Back Hygromycin DsRed-Monomer and AcGFP1 expression vectors Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins. Back 632444: pZsYellow1-C1 Vector
632443	pZsYellow Vector	20 ug	USD $589.00
pZsYellow is a prokaryotic expression vector, which encodes Zoanthus sp. yellow fluorescent protein (ZsYellow). The ZsYellow coding sequence carried in pZsYellow retains the wild-type codon usage. In this vector, the ZsYellow gene is flanked by separate and distinct MCS regions at the 5' and 3' ends so that the ZsYellow gene can be subcloned into other expression vectors. This vector is primarily intended to serve as a convenient source of ZsYellow cDNA. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Basic fluorescent protein vector map Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning. Back Four-color visualization of Living Colors Fluorescent Proteins Four-color visualization of Living Colors Fluorescent Proteins. HEK 293 clonal cell lines stably expressing AmCyan1, ZsGreen1, ZsYellow1, or HcRed1 were mixed and plated in the same culture dish and imaged at 20X magnification. Back Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1 Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1. Back 632443: pZsYellow Vector
630050	Reef Coral Fluorescent Protein Vector Set	Each	USD $5444.00
A set of twelve vectors encoding novel fluorescent proteins in four colors: cyan, green, red and yellow. Four constructs are prokaryotic expression vectors that also serve as convenient sources of fluorescent protein cDNAs. In each of these prokaryotic vectors, the full-length cDNA is flanked by distinct MCSs to facilitate excision from the vector and insertion into other eukaryotic or prokaryotic expression constructs. Eight of the vectors are designed for studies in mammalian systems. These vectors allow expression of a protein of interest as a C- or N-terminal fusion to any of the four fluorescent proteins. The fusion vectors can also be used as cotransfection markers; the unmodified vectors will express fluorescent protein. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like

Overview

Bright yellow fluorescent protein
ZsYellow1 excitation and emission maxima are 529 and 539 nm, respectively

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Applications

Fusions
Protein localization
General reporter for mammalian cells
Monitoring transfection efficiencies

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Fluorescent protein related links

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ZsYellow1 fluorescent protein

Notice to purchaser

Schematic for fluorescent fusion protein vectors

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

632445: pZsYellow1-N1 Vector

Notice to purchaser

Schematic for fluorescent fusion protein vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors

632444: pZsYellow1-C1 Vector

Notice to purchaser

Basic fluorescent protein vector map

Four-color visualization of Living Colors Fluorescent Proteins

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

632443: pZsYellow Vector

Notice to purchaser

Overview

More Information

Applications

Additional product information

Fluorescent protein related links