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Fluorescent protein plasmids

ZsYellow1 fluorescent protein

ZsYellow1 has a true yellow emission, making it ideal for multicolor applications. Like all of our Living Colors fluorescent proteins, ZsYellow1 can be detected in cells without adding cofactors or substrates, which makes it ideal for use in live cell assays. The gene for ZsYellow1 has been human codon-optimized to enhance its translation in mammalian cells, and for higher solubility, brighter emission, and rapid chromophore maturation (8–12 hours).

The ZsYellow1 fluorescent protein can be used as a molecular tag or as a reporter to visualize, track, and quantify cellular processes including protein synthesis and turnover, protein translocation, gene induction, and cell lineage. Because its excitation and emission spectra are distinct from our other fluorescent proteins, ZsYellow1 can be used for multiplex applications—that is, to simultaneously detect two or more events in the same cell or cell population. For example, it can be used in two- and three-color analyses with AmCyan1 and/or HcRed1. Like all of our Living Colors fluorescent proteins, ZsYellow1 is ideal for monitoring gene expression and protein localization in vivo, in situ, and in real time.

We also offer a polyclonal anti-ZsYellow1 antibody.

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Cat. #

Product

Size

Price

License

Quantity

Details

632443

pZsYellow Vector

20 ug

$589.00

License Statement

ID Number

Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pZsYellow Vector product License Agreement can be found by clicking here.

The RCFPs (including DsRedExpress, DsRedExpress2, and E2-Crimson) are covered by one or more of the following U.S. Patent Nos. 7,166,444; 7,157,565; 7,217,789; 7,338,784; 7,338,783; 7,537,915; 6,969,597; 7,150,979; 7,442,522 and 8,012,682.

pZsYellow is a prokaryotic expression vector, which encodes Zoanthus sp. yellow fluorescent protein (ZsYellow). The ZsYellow coding sequence carried in pZsYellow retains the wild-type codon usage. In this vector, the ZsYellow gene is flanked by separate and distinct MCS regions at the 5' and 3' ends so that the ZsYellow gene can be subcloned into other expression vectors. This vector is primarily intended to serve as a convenient source of ZsYellow cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

632444

pZsYellow1-C1 Vector

20 ug

$589.00

License Statement

ID Number

Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pZsYellow1-C1 Vector product License Agreement can be found by clicking here.

pZsYellow1-C1 is a human codon-optimized expression vector that encodes a variant of the Zoanthus sp. yellow fluorescent protein, ZsYellow1, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pZsYellow1-C1 allows genes cloned into the multiple cloning site (MCS) downstream of the ZsYellow1 coding sequence to be expressed as fusions to the C-terminus of the fluorescent protein. The unmodified vector will express ZsYellow1 in mammalian cells.

Notice to purchaser

Components

632445

pZsYellow1-N1 Vector

20 ug

$589.00

License Statement

ID Number

Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pZsYellow1-N1 Vector product License Agreement can be found by clicking here.

pZsYellow1-N1 is a human codon-optimized expression vector that encodes a variant of the Zoanthus sp. yellow fluorescent protein, ZsYellow1, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pZsYellow1-N1 allows genes cloned into the multiple cloning site (MCS) upstream of the ZsYellow1 coding sequence to be expressed as fusions to the N-terminus of the fluorescent protein. The unmodified vector will express ZsYellow1 in mammalian cells.

Notice to purchaser

Components

630050

Reef Coral Fluorescent Protein Vector Set

Each

$5444.00

License Statement

ID Number

Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the Reef Coral Fluorescent Protein Vector Set product License Agreement can be found by clicking here.

A set of twelve vectors encoding novel fluorescent proteins in four colors: cyan, green, red and yellow. Four constructs are prokaryotic expression vectors that also serve as convenient sources of fluorescent protein cDNAs. In each of these prokaryotic vectors, the full-length cDNA is flanked by distinct MCSs to facilitate excision from the vector and insertion into other eukaryotic or prokaryotic expression constructs. Eight of the vectors are designed for studies in mammalian systems. These vectors allow expression of a protein of interest as a C- or N-terminal fusion to any of the four fluorescent proteins. The fusion vectors can also be used as cotransfection markers; the unmodified vectors will express fluorescent protein.

Notice to purchaser

Components

*You must be logged in to a Business Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

Bright yellow fluorescent protein
ZsYellow1 excitation and emission maxima are 529 and 539 nm, respectively

More Information

Applications

Fusions
Protein localization
General reporter for mammalian cells
Monitoring transfection efficiencies

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Fluorescent protein related links

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2018 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

ZsYellow1 fluorescent protein

Notice to purchaser

Basic fluorescent protein vector map

Four-color visualization of Living Colors Fluorescent Proteins

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Notice to purchaser

Schematic for fluorescent fusion protein vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors

Notice to purchaser

Schematic for fluorescent fusion protein vectors

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Notice to purchaser

Overview

More Information

Applications

Additional product information

Fluorescent protein related links