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Home › Products › Gene function › Fluorescent proteins › Fluorescent protein plasmids › Orange and yellow fluorescent proteins › ZsYellow1 fluorescent protein

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ZsYellow1 fluorescent protein

Yellow fluorescent protein vectors

ZsYellow1 has a true yellow emission, making it ideal for multicolor applications. Like all of our Living Colors fluorescent proteins, ZsYellow1 can be detected in cells without adding cofactors or substrates, which makes it ideal for use in live cell assays. The gene for ZsYellow1 has been human codon-optimized to enhance its translation in mammalian cells, and for higher solubility, brighter emission, and rapid chromophore maturation (8–12 hours).

ZsYellow1 has a true yellow emission, making it ideal for multicolor applications. Like all of our Living Colors fluorescent proteins, ZsYellow1 can be detected in cells without adding cofactors or substrates, which makes it ideal for use in live cell assays. The gene for ZsYellow1 has been human codon-optimized to enhance its translation in mammalian cells, and for higher solubility, brighter emission, and rapid chromophore maturation (8–12 hours).

The ZsYellow1 fluorescent protein can be used as a molecular tag or as a reporter to visualize, track, and quantify cellular processes including protein synthesis and turnover, protein translocation, gene induction, and cell lineage. Because its excitation and emission spectra are distinct from our other fluorescent proteins, ZsYellow1 can be used for multiplex applications—that is, to simultaneously detect two or more events in the same cell or cell population. For example, it can be used in two- and three-color analyses with AmCyan1 and/or HcRed1. Like all of our Living Colors fluorescent proteins, ZsYellow1 is ideal for monitoring gene expression and protein localization in vivo, in situ, and in real time.

We also offer a polyclonal anti-ZsYellow1 antibody.

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Cat. # Product Size License Quantity Details
632445 pZsYellow1-N1 Vector 20 ug USD $589.00

pZsYellow1-N1 is a human codon-optimized expression vector that encodes a variant of the Zoanthus sp. yellow fluorescent protein, ZsYellow1, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pZsYellow1-N1 allows genes cloned into the multiple cloning site (MCS) upstream of the ZsYellow1 coding sequence to be expressed as fusions to the N-terminus of the fluorescent protein. The unmodified vector will express ZsYellow1 in mammalian cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Schematic for fluorescent fusion protein vectors

Schematic for fluorescent fusion protein vectors

Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.

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Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2,  and HcRed1.

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632445: pZsYellow1-N1 Vector

632445: pZsYellow1-N1 Vector
632444 pZsYellow1-C1 Vector 20 ug USD $589.00

pZsYellow1-C1 is a human codon-optimized expression vector that encodes a variant of the Zoanthus sp. yellow fluorescent protein, ZsYellow1, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pZsYellow1-C1 allows genes cloned into the multiple cloning site (MCS) downstream of the ZsYellow1 coding sequence to be expressed as fusions to the C-terminus of the fluorescent protein. The unmodified vector will express ZsYellow1 in mammalian cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Schematic for fluorescent fusion protein vectors

Schematic for fluorescent fusion protein vectors

Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.

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Hygromycin DsRed-Monomer and AcGFP1 expression vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins.

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632444: pZsYellow1-C1 Vector

632444: pZsYellow1-C1 Vector
632443 pZsYellow Vector 20 ug USD $589.00

pZsYellow is a prokaryotic expression vector, which encodes Zoanthus sp. yellow fluorescent protein (ZsYellow). The ZsYellow coding sequence carried in pZsYellow retains the wild-type codon usage. In this vector, the ZsYellow gene is flanked by separate and distinct MCS regions at the 5' and 3' ends so that the ZsYellow gene can be subcloned into other expression vectors. This vector is primarily intended to serve as a convenient source of ZsYellow cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Basic fluorescent protein vector map

Basic fluorescent protein vector map

Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.

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Four-color visualization of Living Colors Fluorescent Proteins

Four-color visualization of Living Colors Fluorescent Proteins
Four-color visualization of Living Colors Fluorescent Proteins. HEK 293 clonal cell lines stably expressing AmCyan1, ZsGreen1, ZsYellow1, or HcRed1 were mixed and plated in the same culture dish and imaged at 20X magnification.

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Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2,  and HcRed1.

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632443: pZsYellow Vector

632443: pZsYellow Vector
630050 Reef Coral Fluorescent Protein Vector Set Each USD $5444.00

A set of twelve vectors encoding novel fluorescent proteins in four colors: cyan, green, red and yellow. Four constructs are prokaryotic expression vectors that also serve as convenient sources of fluorescent protein cDNAs. In each of these prokaryotic vectors, the full-length cDNA is flanked by distinct MCSs to facilitate excision from the vector and insertion into other eukaryotic or prokaryotic expression constructs. Eight of the vectors are designed for studies in mammalian systems. These vectors allow expression of a protein of interest as a C- or N-terminal fusion to any of the four fluorescent proteins. The fusion vectors can also be used as cotransfection markers; the unmodified vectors will express fluorescent protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like

Selection guides Fluorescent protein vector finder
Selection guides Fluorescent protein quick guide

Overview

  • Bright yellow fluorescent protein
  • ZsYellow1 excitation and emission maxima are 529 and 539 nm, respectively

More Information

Applications

  • Fusions
  • Protein localization
  • General reporter for mammalian cells
  • Monitoring transfection efficiencies

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Fluorescent protein related links

  • Fluorescent protein quick guide
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  • Adenovirus vectors with fluorescent proteins
  • Tetracycline-inducible systems with fluorescent proteins

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