Fluorescent protein plasmids
mPlum fluorescent protein
Far-red proteins such as mPlum avoid the natural green autofluorescence found in plants and animals and are preferred for in vivo imaging. Living Colors mPlum is a fruit fluorescent protein which was developed in Dr. Roger Tsien’s lab (Shaner et al. 2004; Wang et al. 2004; Shu et al. 2006) by directed mutagenesis of a monomeric mutant of DsRed (Campbell et al. 2002). It has demonstrated stable expression and performed successfully in numerous fusion applications.
- Far-red fluorescent protein for in vivo imaging
- mPlum excitation and emission maxima: 590 and 649 nm, respectively
- See which antibodies can be use to detect mPlum on Western blot or ICC
- In vivo imaging
- Protein localization studies
Campbell, R. E. et al. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 99, 7877-82 (2002).
Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567–72 (2004).
Shu, X., Shaner, N. C., Yarbrough, C. A., Tsien, R. Y. & Remington, S. J. Novel chromophores and buried charges control color in mFruits. Biochemistry 45, 9639–9647 (2006).
Wang, L., Jackson, W. C., Steinbach, P. a & Tsien, R. Y. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc. Natl. Acad. Sci. U. S. A. 101, 16745–16749 (2004).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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