mPlum fluorescent protein
Far-red proteins such as mPlum avoid the natural green autofluorescence found in plants and animals and are preferred for in vivo imaging. Living Colors mPlum is a fruit fluorescent protein which was developed in Dr. Roger Tsien’s lab (Shaner et al. 2004; Wang et al. 2004; Shu et al. 2006) by directed mutagenesis of a monomeric mutant of DsRed (Campbell et al. 2002). It has demonstrated stable expression and performed successfully in numerous fusion applications.
- Far-red fluorescent protein for in vivo imaging
- mPlum excitation and emission maxima: 590 and 649 nm, respectively
- See which antibodies can be use to detect mPlum on Western blot or ICC
- In vivo imaging
- Protein localization studies
Campbell, R. E. et al. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 99, 7877-82 (2002).
Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567–72 (2004).
Shu, X., Shaner, N. C., Yarbrough, C. A., Tsien, R. Y. & Remington, S. J. Novel chromophores and buried charges control color in mFruits. Biochemistry 45, 9639–9647 (2006).
Wang, L., Jackson, W. C., Steinbach, P. a & Tsien, R. Y. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc. Natl. Acad. Sci. U. S. A. 101, 16745–16749 (2004).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Easily design primers for In-Fusion Cloning
Our NEW In-Fusion Cloning Primer Design Tool allows for single- or multiple-insert cloning, accommodates vector linearization by inverse PCR or restriction digest, and enables site-directed mutagenesis. Simply input the DNA sequences of your vector and insert(s), along with your linearization method to generate primers for your next cloning experiment. Easily switch to the mutagenesis option to generate primers for all of your insertion, replacement, and deletion projects.Design your primers
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