Timer fluorescent protein

Photoactivatable/switchable fluorescent protein

Study promoter activity using the Living Colors fluorescent Timer, a fluorescent protein that shifts color from green to red over time—in a matter of hours, depending on the expression system (Terskikh et al. 2000). Shortly after its synthesis, the fluorescent Timer begins emitting green fluorescence, but as time passes, the fluorophore undergoes additional changes that shift its fluorescence to longer wavelengths. When fully matured, the protein is bright red.

Study promoter activity using the Living Colors fluorescent Timer, a fluorescent protein that shifts color from green to red over time—in a matter of hours, depending on the expression system (Terskikh et al. 2000). Shortly after its synthesis, the fluorescent Timer begins emitting green fluorescence, but as time passes, the fluorophore undergoes additional changes that shift its fluorescence to longer wavelengths. When fully matured, the protein is bright red. Detect the Living Colors fluorescent Timer with the DsRed polyclonal antibody.

More Information

Applications

  • Promoter studies
  • Visualize the location and time frame of promoter activity (e.g., during embryogenesis and cell differentiation)
References

Terskikh, A. et al. "Fluorescent timer": protein that changes color with time. Science 290(5496):1585–1588 (2000).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.