Timer fluorescent protein

Photoactivatable/switchable fluorescent protein

Study promoter activity using the Living Colors fluorescent Timer, a fluorescent protein that shifts color from green to red over time—in a matter of hours, depending on the expression system (Terskikh et al. 2000). Shortly after its synthesis, the fluorescent Timer begins emitting green fluorescence, but as time passes, the fluorophore undergoes additional changes that shift its fluorescence to longer wavelengths. When fully matured, the protein is bright red.

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Cat. #	Product	Size
632403	pTimer-1 Vector	20 ug	USD $589.00
pTimer-1 is a promoterless vector that can be used to monitor transcription from different promoters and promoter/enhancer combinations inserted into the MCS located upstream of the DsRed1-E5 coding sequence. Shortly after translation, DsRed1-E5 emits green light; its red fluorophore emerges later, hours after translation. Because of its predictable color shift, DsRed1-E5 acts as a timer, useful for monitoring changes in gene activity in living cells. pTimer-1 can be used to study the on-off activity of any cis-acting regulatory element cloned into the MCS upstream of DsRed1-E5. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Vector map for pTimer vectors Vector map for pTimer vectors. As the Fluorescent Timer matures, it changes color—in a matter of hours, depending on the expression system used. Back Fluorescent timer under the control of the heat shock promoter hsp16-41 in a transgenic C. elegans embryo *Fluorescent Timer under the control of the heat shock promoter hsp16-41 in a transgenic C. elegans* embryo.** The embryo was heat shocked in a 33°C water bath. Promoter activity was studied during the heat shock recovery period. Green fluorescence was observed in the embryo as early as two hr into the recovery period. By 50 hr after heat shock, promoter activity had ceased, as indicated by the lack of green color. Back 632403: pTimer-1 Vector
632402	pTimer Vector	20 ug	USD $589.00
pTimer is a prokaryotic expression vector that encodes DsRed1-E5, a fluorescent protein that changes color as it ages. Shortly after translation, DsRed1-E5 emits green light; its red fluorophore emerges later, hours after translation. Because of its predictable color shift, DsRed1-E5 acts as a timer, useful for monitoring changes in gene activity in living cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Vector map for pTimer vectors Vector map for pTimer vectors. As the Fluorescent Timer matures, it changes color—in a matter of hours, depending on the expression system used. Back Fluorescent timer under the control of the heat shock promoter hsp16-41 in a transgenic C. elegans embryo *Fluorescent Timer under the control of the heat shock promoter hsp16-41 in a transgenic C. elegans* embryo.** The embryo was heat shocked in a 33°C water bath. Promoter activity was studied during the heat shock recovery period. Green fluorescence was observed in the embryo as early as two hr into the recovery period. By 50 hr after heat shock, promoter activity had ceased, as indicated by the lack of green color.

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Applications

Promoter studies
Visualize the location and time frame of promoter activity (e.g., during embryogenesis and cell differentiation)

References

Terskikh, A. et al. "Fluorescent timer": protein that changes color with time. Science 290(5496):1585–1588 (2000).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Fluorescent protein related links

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Timer fluorescent protein

Notice to purchaser

Vector map for pTimer vectors

Fluorescent timer under the control of the heat shock promoter hsp16-41 in a transgenic C. elegans embryo

632403: pTimer-1 Vector