Timer fluorescent protein

Photoactivatable/switchable fluorescent protein

Study promoter activity using the Living Colors fluorescent Timer, a fluorescent protein that shifts color from green to red over time—in a matter of hours, depending on the expression system (Terskikh et al. 2000). Shortly after its synthesis, the fluorescent Timer begins emitting green fluorescence, but as time passes, the fluorophore undergoes additional changes that shift its fluorescence to longer wavelengths. When fully matured, the protein is bright red.

More Less

Cat. #

Product

Size

Price

License

Quantity

Details

632402

pTimer Vector

20 ug

USD $589.00

License Statement

ID Number
24	DsRed2, DsRedExpress, DsRedExpress2, E2-Crimson, and HcRed are covered by one or more of the following U.S. Patent Nos. 7,150,979; 7,157,565; 7,183,399; 7,858,844; and non-U.S. international equivalents in some countries.

pTimer is a prokaryotic expression vector that encodes DsRed1-E5, a fluorescent protein that changes color as it ages. Shortly after translation, DsRed1-E5 emits green light; its red fluorophore emerges later, hours after translation. Because of its predictable color shift, DsRed1-E5 acts as a timer, useful for monitoring changes in gene activity in living cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

632403

pTimer-1 Vector

20 ug

USD $589.00

License Statement

ID Number
24	DsRed2, DsRedExpress, DsRedExpress2, E2-Crimson, and HcRed are covered by one or more of the following U.S. Patent Nos. 7,150,979; 7,157,565; 7,183,399; 7,858,844; and non-U.S. international equivalents in some countries.

pTimer-1 is a promoterless vector that can be used to monitor transcription from different promoters and promoter/enhancer combinations inserted into the MCS located upstream of the DsRed1-E5 coding sequence. Shortly after translation, DsRed1-E5 emits green light; its red fluorophore emerges later, hours after translation. Because of its predictable color shift, DsRed1-E5 acts as a timer, useful for monitoring changes in gene activity in living cells. pTimer-1 can be used to study the on-off activity of any cis-acting regulatory element cloned into the MCS upstream of DsRed1-E5.

Notice to purchaser

Components

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

More Information

Applications

Promoter studies
Visualize the location and time frame of promoter activity (e.g., during embryogenesis and cell differentiation)

References

Terskikh, A. et al. "Fluorescent timer": protein that changes color with time. Science 290(5496):1585–1588 (2000).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Fluorescent protein related links

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2021 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.