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  • ‹ Back to Photoactivatable and photoswitchable proteins
  • Dendra2 fluorescent protein
  • Timer fluorescent protein
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Selection guides Fluorescent protein quick guide
Home › Products › Gene function › Fluorescent proteins › Fluorescent protein plasmids › Photoactivatable and photoswitchable proteins › PAmCherry fluorescent protein

Fluorescent protein plasmids

  • Cyan and green fluorescent proteins
    • AcGFP1 fluorescent protein
    • ZsGreen1 fluorescent protein
    • GFP & GFPuv fluorescent proteins
    • AmCyan1 fluorescent protein
  • Red fluorescent proteins
    • mCherry fluorescent protein
    • DsRed-Monomer fluorescent protein
    • DsRed2 fluorescent protein
    • DsRed-Express and DsRed-Express2 fluorescent proteins
    • tdTomato fluorescent protein
    • AsRed2 fluorescent protein
    • mStrawberry fluorescent protein
  • Far-red fluorescent proteins
    • E2-Crimson fluorescent protein
    • HcRed1 fluorescent protein
    • mRaspberry fluorescent protein
    • mPlum fluorescent protein
  • Orange and yellow fluorescent proteins
    • mOrange2 fluorescent protein
    • mBanana fluorescent protein
    • ZsYellow1 fluorescent protein
  • Photoactivatable and photoswitchable proteins
    • Dendra2 fluorescent protein
    • Timer fluorescent protein
    • PAmCherry fluorescent protein
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Selection guides Fluorescent protein quick guide

Photoactivatable mCherry fluorescent protein

Photoactivatable/switchable

Living Colors PAmCherry is a photoactivatable fluorescent protein (PAFP) derived from the red fluorescent protein mCherry. PAmCherry is non-fluorescent until it is exposed to 350- to 400-nm light. By selecting which cells or cellular regions to activate, you can track cells, organelles, or proteins of interest against a dark background using this photoactivatable fluorescent protein.

Living Colors PAmCherry is a photoactivatable fluorescent protein (PAFP) derived from the red fluorescent protein mCherry. PAmCherry is non-fluorescent until it is exposed to 350- to 400-nm light. By selecting which cells or cellular regions to activate, you can track cells, organelles, or proteins of interest against a dark background using this photoactivatable fluorescent protein. The activated PAmCherry excitation maximum is 564 nm, and the emission maximum is 595 nm, which allows you to visualize and monitor photoactivated PAmCherry with the same filter sets used for other red fluorescent proteins, such as DsRed variants and mCherry.

Photoactivatable fluorescent proteins such as PAmCherry are particularly useful for determining protein half-life and protein transport pathways because molecules synthesized after activation are not able to fluoresce. Therefore, what you observe is a snapshot of the protein molecules that were present at the time of activation.

PAmCherry subcellular localization vectors are available that target PAmCherry to the cell membrane, mitochondria, actin, or tubulin, respectively. Plasmid and lentiviral N- and C-terminal vectors are also available to create your own PAmCherry-tagged protein of interest.

PAmCherry sequence files can be found in the Documents tab by expanding Details within the Product Table below.

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Cat. # Product Size License Quantity Details
632591 pPAmCherry-Mito Vector 10 ug USD $589.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
69 The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pPAmCherry-Mito Vector product License Agreement can be found by clicking here.
330 This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

pPAmCherry-Mito Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry, fused to the mitochondrial targeting sequence derived from the precursor subunit VIII of human cytochrome C oxidase. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

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Photoactivated PAmCherry-Mito shows strong red fluorescence and localizes correctly to the mitochondria

Photoactivated PAmCherry-Mito shows strong red fluorescence and localizes correctly to the mitochondria

Photoactivated PAmCherry-Mito shows strong red fluorescence and localizes correctly to the mitochondria. U2OS cells were transiently transfected with pPAmCherry-Mito. PAmCherry-Mito was activated using an Argon 458 nm laser (scan speed: 400 Hz), and the cells were imaged using a HeNe 543 nm laser for excitation and a standard red fluorescence emission filter set.

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The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

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Prior to photoactivation, no red fluorescence is detected in a cell expressing PAmCherry-Mito (Panel A)

Prior to photoactivation, no red fluorescence is detected in a cell expressing PAmCherry-Mito (Panel A)
Prior to photoactivation, no red fluorescence is detected in a cell expressing PAmCherry-Mito (Panel A). However, after photoactivation, strong red fluorescence is observed in the subcellular, activated region of the cell (Panel B). U2OS cells were transiently transfected with pPAmCherry-Mito. Cells were imaged prior to activation in order to determine the level of background fluorescence (Panel A). PAmCherry-Mito was then activated in a small region of a cell imaged using a HeNe 543 nm laser for excitation and a standard red fluorescence emission filter set (Panel B).

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PAmCherry-Mito makes it easy to follow the behavior of a subset of mitochondria

PAmCherry-Mito makes it easy to follow the behavior of a subset of mitochondria

PAmCherry-Mito makes it easy to follow the behavior of a subset of mitochondria. Activating the mitochondria in just one region of the cell makes it possible to follow their movements into dark (nonactivated) areas of the cell. U2OS cells were transiently transfected with pPAmCherry-Mito. PAmCherry-Mito was activated in a small region of a cell, and the cells were imaged every 10 sec for 15 min using a HeNe 543 nm laser for excitation and a standard red fluorescence emission filter set.

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PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.

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632591: pPAmCherry-Mito Vector

632591: pPAmCherry-Mito Vector
632590 pPAmCherry-Tubulin Vector 10 ug USD $589.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
69 The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pPAmCherry-Tubulin Vector product License Agreement can be found by clicking here.
330 This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

pPAmCherry-Tubulin Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry, fused to human alpha-tubulin. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

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The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

Back

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.

Back

632590: pPAmCherry-Tubulin Vector

632590: pPAmCherry-Tubulin Vector
632589 pPAmCherry-Actin Vector 10 ug USD $589.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
69 The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pPAmCherry-Actin Vector product License Agreement can be found by clicking here.
330 This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

pPAmCherry-Actin Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry, fused to human cytoplasmic beta-actin. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

Back

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.

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632589: pPAmCherry-Actin Vector

632589: pPAmCherry-Actin Vector
632588 pPAmCherry-Mem Vector 10 ug USD $589.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
69 The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pPAmCherry-Mem Vector product License Agreement can be found by clicking here.
330 This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

pPAmCherry-Mem Vector is mammalian expression vector encoding a fusion protein of PAmCherry and the N-terminal 20 amino acids of neuromodulin (GAP-43). PAmCherry is a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. The GAP-43 fragment contains a signal for posttranslational palmitoylation of cysteins 3 and 4 that targets the fusion protein to the plasma membrane.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

Back

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.

Back

632588: pPAmCherry-Mem Vector

632588: pPAmCherry-Mem Vector
632587 pLVX-PAmCherry-C1 Vector 10 ug USD $693.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
55 cPPT Element. This product and its use are the subject to one or more of the following U.S. Pat. No. 8,093,042. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Takara Bio USA, Inc. is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).
69 The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pLVX-PAmCherry-C1 Vector product License Agreement can be found by clicking here.
330 This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.
K11 This product and its use are the subject to one or more of the following U.S. Pat. 8,093,042 and foreign equivalents. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and com­ponents of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any com­mercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Takara Bio USA is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

pLVX-PAmCherry-C1 Vector is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to infect cells and express your gene of interest fused to PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescent until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Genes cloned into the MCS will be expressed as fusions to the C-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

Back

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
632586 pLVX-PAmCherry-N1 Vector 10 ug USD $693.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
55 cPPT Element. This product and its use are the subject to one or more of the following U.S. Pat. No. 8,093,042. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Takara Bio USA, Inc. is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).
69 The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pLVX-PAmCherry-N1 Vector product License Agreement can be found by clicking here.
330 This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.
K11 This product and its use are the subject to one or more of the following U.S. Pat. 8,093,042 and foreign equivalents. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and com­ponents of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any com­mercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Takara Bio USA is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

pLVX-PAmCherry-N1 Vector is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to infect cells and express your gene of interest fused to PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Genes cloned into the MCS will be expressed as fusions to the N-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

Back

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
632585 pPAmCherry-C1 Vector 10 ug USD $589.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
69 The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pPAmCherry-C1 Vector product License Agreement can be found by clicking here.
330 This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

pPAmCherry-C1 Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescent until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Genes cloned into the MCS will be expressed as fusions to the C-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

Back

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
632584 pPAmCherry-N1 Vector 10 ug USD $589.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
69 The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pPAmCherry-N1 Vector product License Agreement can be found by clicking here.
330 This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

pPAmCherry-N1 Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescent until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Genes cloned into the MCS will be expressed as fusions to the N-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

Back

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.

Selection guides Fluorescent protein quick guide
Antibodies to GFP variants, mCherry and other fluorescent proteins Fluorescent protein antibodies

Overview

  • Switch on the red fluorescent protein mCherry in a subset of cells, proteins, or organelles, in order to track their movement.
  • Non-fluorescent until activated by a short exposure to 350- to 400-nm light
    (This is the optimal range of wavelengths)
  • Excitation maximum: 564 nm
    Emission maximum: 595 nm
  • Image PAmCherry with the same filter sets used to detect red fluorescent proteins such as DsRed and mCherry
  • Detect PAmCherry with the mCherry monoclonal antibody

More Information

Applications

  • Tracking subpopulations of organelles or protein molecules
  • Visualizing real-time protein dynamics (movement, degradation, etc.)
  • Determining protein half-life and protein transport pathways
  • Observing cell division, migration, and morphology

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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  • Purified total RNA and mRNA
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