HcRed1 fluorescent protein

Far red

Living Colors HcRed1 is a far-red fluorescent protein derived from a non-fluorescent chromoprotein found in the sea anemone Heteractis crispa. The coding sequence of the HcRed1 fluorescent protein was modified for fluorescence and is human codon-optimized for enhanced expression in mammalian cells. HcRed1 has a low tendency to form aggregates in living cells and can be detected just 16 hours after transfection by flow cytometry, a time frame which is comparable with EGFP. 

Living Colors HcRed1 is a far-red fluorescent protein derived from a non-fluorescent chromoprotein found in the sea anemone Heteractis crispa. The coding sequence of the HcRed1 fluorescent protein was modified for fluorescence and is human codon-optimized for enhanced expression in mammalian cells. HcRed1 has a low tendency to form aggregates in living cells and can be detected just 16 hours after transfection by flow cytometry, a time frame which is comparable with EGFP. Far-red fluorescent proteins like HcRed1 avoid the natural green autofluorescence found in plants and animals and are preferred for in vivo imaging.

Overview

  • Far-red fluorescent protein, ideal for in vivo imaging
  • HcRed1 fluorescent protein excitation and emission maxima: 588 and 618 nm, respectively
  • HcRed1 is detected on a Western blot using anti-RCFP polyclonal pan antibody

More Information

Applications

  • Fusions
  • Protein localization studies
  • General reporter for mammalian cells
  • Monitoring transfection efficiencies
  • In vivo imaging

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.