632539
pCMV DsRed-Express2 Vector
20 ug
$571.00
*
pCMV DsRed-Express2 is designed for whole cell labeling or to be used as a cotransfection marker in mammalian cells; the red fluorescence of DsRed-Express2 can be detected by fluorescence microscopy or flow cytometry, providing direct visual evidence of transfection or the ability to monitor cells after transplantation experiments in vivo. After cotransfection with pCMV DsRed-Express2 and an expression construct of interest, cells can also be sorted by flow cytometry (FACS) to enrich for transfected cells. pCMV DsRed-Express2 Vector can be transfected into mammalian cells using any standard transfection method. If required, stable transfectants can be selected using G418.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632416
pCMV-DsRed-Express Vector
20 ug
$589.00
*
pCMV-DsRed-Express is a mammalian expression vector designed for use as a cotransfection marker, together with an expression construct of interest. It constitutively expresses the red fluorescent protein DsRed-Express, which can be detected by fluorescence microscopy to provide direct visual evidence of transfection. Cells can also be sorted by flow cytometry to enrich for transfected cells.
pCMV-DsRed-Express Vector can be transfected into mammalian cells using any standard transfection method. If required, stable transfectants can be selected using G418.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632412
pDsRed-Express Vector
20 ug
$589.00
*
pDsRed-Express is a prokaryotic expression vector that encodes DsRed-Express, a variant of Discosoma sp. red fluorescent protein (DsRed). pDsRed-Express is primarily intended to serve as a source of DsRed-Express cDNA. The flanking MCS regions make it possible to excise the DsRed-Express coding sequence and insert it into other expression vectors of choice. The vector can also be used in bacteria to produce DsRed-Express protein.
In pDsRed-Express, the DsRed-Express coding sequence is flanked at the 5' and 3' ends by separate and distinct multiple cloning sites (MCS), making it easy to excise the gene for use in other cloning applications. Alternatively, the DsRed-Express coding sequence can be amplified by PCR. In E. coli, DsRed-Express is expressed from the lac promoter as a fusion with several amino acids, including the first five amino acids of the LacZ protein. Note, however, that if you excise the DsRed-Express coding sequence using a restriction site in the 5' MCS, the resulting fragment will encode the native (i.e., non-fusion) DsRed-Express protein. A Kozak consensus sequence is located immediately upstream of the DsRed-Express gene to enhance translational efficiency in eukaryotic systems. The entire DsRed-Express expression cassette in pDsRed-Express is supported by a pUC19 backbone, which contains a high-copy number origin of replication and an ampicillin resistance gene for propagation and selection in E. coli.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632413
pDsRed-Express-1 Vector
20 ug
$589.00
*
pDsRed-Express-1 is a promoterless mammalian expression vector that can be used to monitor transcription from different promoters and promoter/enhancer combinations inserted into the multiple cloning site (MCS). It encodes DsRed-Express, a variant of Discosoma sp. red fluorescent protein (DsRed). DsRed-Express contains nine amino acid substitutions which improve the solubility of the protein, reduce the time from transfection to detection of red fluorescence, and decrease the level of residual green emission. When DsRed-Express is expressed in mammalian cell cultures, red-emitting cells can be detected by either fluorescence microscopy or flow cytometry 8-12 hours after transfection. Although DsRed-Express most likely forms the same tetrameric structure as wild-type DsRed, DsRed-Express displays a reduced tendency to aggregate. The DsRed-Express coding sequence is human codon-optimized for high expression in mammalian cells.
The sequence upstream of the DsRed-Express gene has been converted to a Kozak consensus sequence to enhance translation efficiency in eukaryotic cells. SV40 polyadenylation signals downstream of the DsRed-Express gene direct proper processing of the 3' end of the DsRed-Express mRNA. The vector backbone contains an SV40 origin for replication in mammalian cells expressing the SV40 T antigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin-resistance cassette (Neor ) allows stably transfected eukaryotic cells to be selected using G418. This cassette consists of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene. A bacterial promoter upstream of the cassette expresses kanamycin resistance in E. coli.
Promoters should be cloned into the pDsRed-Express-1 MCS upstream from the DsRed-Express coding sequence. Without addition of a functional promoter, this vector will not express DsRed-Express. The recombinant DsRed-Express vector can be transfected into mammalian cells using any standard transfection method. If required, stable transfectants can be selected using G418.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632535
pDsRed-Express2 Vector
20 ug
$571.00
*
pDsRed-Express2 is a prokaryotic expression vector that encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein. pDsRed-Express 2 is primarily intended to serve as a source of DsRed-Express 2 cDNA. The flanking MCS regions make it possible to excise the DsRed-Express2 coding sequence and insert it into other expression vectors of choice. The vector can also be used in bacteria to produce DsRed-Express2 protein.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632536
pDsRed-Express2-1 Vector
20 ug
$571.00
*
pDsRed-Express2-1 encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein. pDsRed-Express2-1 is a promoterless vector that can be used to monitor transcription from different promoters and promoter/enhancer combinations inserted into the multiple cloning site (MCS) upstream of the promoterless DsRed-Express2 gene. Without the addition of a functional promoter, this vector will not express DsRed-Express2.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632538
pDsRed-Express2-C1 Vector
20 ug
$571.00
*
pDsRed-Express2-C1 encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein. It can be used to construct fusions to the C-terminus of DsRed-Express2 which retain the fluorescent properties of the native DsRed-Express2 protein. Its expression can be monitored by flow cytometry, and its intracellular localization can be determined by fluorescence microscopy. pDsRed-Express2-C1 can also be used as a cotransfection marker; the unmodified vector will express DsRed-Express2.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632537
pDsRed-Express2-N1 Vector
20 ug
$571.00
*
pDsRed-Express2-N1 is a mammalian expression vector that encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein (DsRed). pDsRed-Express2-N1 can be used to construct fusions to the N-terminus of DsRed-Express2. The fusion construct retains the fluorescent properties of the native DsRed-Express2 protein, and its expression can be monitored by flow cytometry and its cellular localization can be determined by fluorescence microscopy. pDsRed-Express2-N1 can also be used as a cotransfection marker; the unmodified vector will express DsRed-Express2 in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632430
pDsRed-Express-C1 Vector
20 ug
$589.00
*
pDsRed-Express-C1 encodes DsRed-Express, a variant of Discosoma sp. red fluorescent protein (DsRed). DsRed-Express contains nine amino acid substitutions (as compared to DsRed1), which improve the solubility, accelerate the maturation, and decrease the level of residual green emission. pDsRed-Express allows genes cloned into the multiple cloning site (MCS) downstream of the DsRed-Express coding sequence to be expressed as fusions to the C-terminus of DsRed-Express. The unmodified vector will express DsRed-Express in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632423
pDsRed-Express-DR Vector
20 ug
$589.00
*
pDsRed-Express-DR is a promoterless vector that encodes DsRed-Express-DR, a destabilized variant of Discosoma sp. red fluorescent protein, DsRed-Express. DsRed-Express-DR contains a PEST degradation domain at its C-terminus, so it is rapidly turned over in mammalian cell culture. Because if its short half-life, DsRed-Express-DR is well suited for monitoring the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site, located upstream of the human codon-optimized DsRed-Express-DR gene. Without the addition of a functional promoter, this vector will not express DsRed-Express-DR.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632429
pDsRed-Express-N1 Vector
20 ug
$589.00
*
pDsRed-Express-N1 encodes DsRed-Express, a variant of Discosoma sp. red fluorescent protein (DsRed). DsRed-Express contains nine amino acid substitutions (as compared to DsRed1), which improve the solubility, increase the maturation rate, and decrease the level of residual green emission. pDsRed-Express allows genes cloned into the multiple cloning site (MCS) upstream of the DsRed-Express coding sequence to be expressed as fusions to the N-terminus of DsRed-Express. The unmodified vector will express DsRed-Express in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631979
pEF1alpha-DsRed-Express2 Vector
10 ug
$555.00
*
pEF1α-DsRed-Express2 is a mammalian expression vector that constitutively expresses the red fluorescent protein DsRed-Express2, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of DsRed-Express2 is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows the protein to be expressed without the transgene silencing associated with CMV promoters. The vector, which lacks an MCS, is designed to be used for cell labeling or as a marker of transfection efficiency.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631980
pEF1alpha-IRES-DsRed-Express2 Vector
10 ug
$555.00
*
pEF1α-IRES-DsRed-Express2 is a bicistronic mammalian expression vector that allows the simultaneous, constitutive expression of a protein of interest and the red fluorescent protein DsRed-Express2. Expression of the bicistronic transcript is driven by the human elongation factor 1 alpha (EF1α) promoter.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632540
pIRES2 DsRed-Express2 Vector
20 ug
$571.00
*
pIRES2 DsRed-Express 2 is a eukaryotic expression vector that encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV) between the MCS and red fluorescent protein DsRed-Express2 coding region. This permits both the gene of interest (cloned into the MCS) and the DsRed-Express2 gene to be translated from a single bicistronic mRNA.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632559
pLVX-DsRed-Express2-C1 Vector
10 ug
$676.00
*
This lentiviral expression vector encodes a DsRed-Express2 fluorescent protein tag. The protein is a rapidly maturing tetrameric fluorescent protein from Discosoma sp. that has minimal cytotoxicity, high solubility, and exhibits strong and stable expression in mammalian cells. Inserting a cDNA in the MCS downstream of the DsRed-Express2 coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using the Lenti-X Packaging Single Shots (VSV-G) and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632560
pLVX-DsRed-Express2-N1 Vector
10 ug
$676.00
*
This lentiviral expression vector encodes a DsRed-Express2 fluorescent protein tag. The protein is a rapidly maturing tetrameric fluorescent protein from Discosoma sp. that has minimal cytotoxicity, high solubility, and exhibits strong and stable expression mammalian cells. Inserting a cDNA in the MCS upstream of the DsRed-Express2 coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using the Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632521
pRetroX-IRES-DsredExpress Vector
20 ug
$568.00
*
The pRetroX-IRES-DsRedExpress vector is a bicistronic retroviral expression vector containing DsRed-Express as a marker for transfection/infection efficiency. A gene of interest is inserted into the MCS located upstream of the encephalomyocarditis virus (ECMV) internal ribosomal entry site (IRES). The IRES sequence allows the protein of interest and DsRed-Express to be independently translated from the same mRNA transcript. DsRed-Express is a human codon-optimized variant of the Discosoma sp. red fluorescent protein (DsRed) that has been engineered for faster maturation.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632437
rDsRed-Express Protein
100 ug
$361.00
Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed-Express). rDsRed-Express can be used as a standard for protein gels, Western blots, fluorometry, and fluorescence activated cell sorting (FACS). It can also be used as a control for DsRed-Express expression studies and for microinjection into cells and tissues. At its N-terminus, rDsRed-Express contains a 6xHN epitope tag, used for purification of the protein by metal ion affinity chromatagraphy (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and DsRed-Express, makes it possible to remove the 6xHN polypetide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
