ZsGreen1 fluorescent protein

Living Colors ZsGreen1 is an exceptionally bright green fluorescent protein derived from a Zoanthus sp. reef coral (Matz et al. 1999) that has been modified for high solubility, bright emission, and rapid chromophore maturation. ZsGreen1 is the brightest commercially available green fluorescent protein—up to 4X brighter than EGFP—and is ideally suited for whole-cell labelling, promoter-reporter studies, or as a transfection control.

Living Colors ZsGreen1 is an exceptionally bright green fluorescent protein derived from a Zoanthus sp. reef coral (Matz et al. 1999) that has been modified for high solubility, bright emission, and rapid chromophore maturation. ZsGreen1 is the brightest commercially available green fluorescent protein—up to 4X brighter than EGFP—and is ideally suited for whole-cell labelling, promoter-reporter studies, or as a transfection control. The protein naturally forms a tetramer and is therefore not optimal for development of fusion proteins; we recommend AcGFP1, a true monomer, for such applications.

Overview

  • Exceptional brightness: up to 4X brighter than EGFP
  • ZsGreen1 excitation and emission maxima are 493 nm and 505 nm, respectively
  • We offer both monoclonal and polyclonal ZsGreen1 antibodies, which recognize native and denatured forms of ZsGreen1 fluorescent protein, as well as fusion proteins containing ZsGreen1. They do not cross-react with Aequorea sp. GFPs (AcGFP1, EGFP). 
  • Bicistronic (pIRES-) expression vectors are available
  • Lentiviral (pLVX-) and retroviral (pRetroX-) formats are available

More Information

Applications

  • Whole-cell labeling
  • Promoter-reporter studies
  • Transfection controls
References

Matz, M. V. et al. Fluorescent proteins from nonbioluminescent Anthozoa species. Nature Biotechnol. 17, 969–973 (1999).

Additional product information

Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysisand Kit Components Lists are located under the Documents tab.