pmR-ZsGreen1 Vector

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Cat. # Product Size Price License Quantity Details
632475 Living Colors® Anti-RCFP Polyclonal Pan Antibody 100 uL USD $410.00

The Living Colors Anti-RCFP Polyclonal Pan Antibody was raised in a rabbit against recombinant full-length Zoanthus sp. yellow fluorescent protein (ZsYellow). This pan antibody recognizes denatured forms of AmCyan, ZsGreen, ZsYellow, DsRed2, DsRed-Express, HcRed, AsRed, as well as fusion proteins containing these RCFP variants in bacterial and mammalian cell lysates.

632474 Living Colors® Full-Length ZsGreen Polyclonal Antibody 100 uL USD $410.00

The Living Colors Full-Length ZsGreen Polyclonal Antibody was raised in a rabbit against recombinant full-length Zoanthus sp. green fluorescent protein (ZsGreen). This antibody recognizes native and denatured forms of ZsGreen as well as fusion proteins containing ZsGreen in bacterial and mammalian cell lysates.

631120 Mir-X™ Inducible miRNA System (Green) Each USD $797.00

The Mir-X Inducible miRNA System (Green) contains a set of expression vectors that allows you to establish a Tet-regulated microRNA (miRNA) expression system in mammalian cells. An additional feature of the system is that a bright green fluorescent protein marker, ZsGreen1, is coexpressed along with your miRNA, enabling you to verify miRNA expression in intact cells. Inducible expression of the ZsGreen1/pre-miRNA transcript is accomplished by creating a double-stable cell line that expresses the Tet-On Advanced transactivator and that bears your customized pmRi-ZsGreen1 Vector, and then culturing the cell line in the presence of the tetracycline derivative, doxycycline.

631118 Mir-X™ Inducible miRNA System (Red) Each USD $797.00

The Mir-X Inducible miRNA System (Red) is a set of expression vectors that allows you to establish a Tet-regulated microRNA (miRNA) expression system in mammalian cells. An additional feature of the system is that a bright red fluorescent protein marker, mCherry, is coexpressed along with your miRNA, enabling you to verify miRNA expression in intact cells. Inducible expression of the mCherry/pre-miRNA transcript is accomplished by creating a double-stable cell line that expresses the Tet-On Advanced transactivator and that bears your customized pmRi-mCherry/miRNA vector, and then culturing the cell line in the presence of the tetracycline derivative, doxycycline.

638314 Mir-X™ miRNA qRT-PCR TB Green® Kit 200 Rxns USD $422.00

This 200 rxn kit enables you to synthesize first-strand cDNA from either total RNA or purified small RNAs isolated from any source, and use the resulting cDNA to quantify specific miRNAs and other RNAs present in the sample. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The TB Green Advantage qPCR Premix and real-time qPCR are used to quantify the sequences of choice in the cDNA. For 600 rxn kit see Cat. # 638316.