Fluorescent protein plasmids
mRaspberry fluorescent protein
Far-red fluorescent proteins such as mRaspberry, mPlum, E2-Crimson, and HcRed1 avoid the natural green autofluorescence found in plants and animals and are ideal for in vivo imaging. mRaspberry is a fruit fluorescent protein which was developed in Dr. Roger Tsien's lab (Shaner et al. 2004; Wang et al. 2004; Shu et al. 2006) by directed mutagenesis of a monomeric mutant of DsRed (Campbell et al. 2002). mRaspberry shows stable expression and great performance in applications using fusion proteins.
- Far-red fluorescent protein ideal for in vivo applications
- mRaspberry fluorescent protein excitation and emission maxima: 598 and 625 nm, respectively
- Detect mRaspberry on a Western blot using these antibodies
- Protein localization studies
- In vivo imaging
Campbell, R. E. et al. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 99, 7877–82 (2002).
Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567–72 (2004).
Shu, X., Shaner, N. C., Yarbrough, C. A., Tsien, R. Y. & Remington, S. J. Novel chromophores and buried charges control color in mFruits. Biochemistry 45, 9639–9647 (2006).
Wang, L., Jackson, W. C., Steinbach, P. a & Tsien, R. Y. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc. Natl. Acad. Sci. U. S. A. 101, 16745–16749 (2004).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Fluorescent protein related links
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