Retroviral vectors with fluorescent proteins

Retroviral vectors for co-expression of fluorescent proteins

These retroviral vectors allow you to deliver and stably express your gene of interest and a fluorescent protein in a broad range of dividing cells. Depending on which vector you choose, the fluorescent protein and protein of interest can be:

  • Fused together for subcellular localization or tag studies
  • Simultaneously coexpressed from the same transcript but as separate proteins (IRES vectors)

These retroviral vectors allow you to deliver and stably express your gene of interest and a fluorescent protein in a broad range of dividing cells. Depending on which vector you choose, the fluorescent protein and protein of interest can be:

  • Fused together for subcellular localization or tag studies
  • Simultaneously coexpressed from the same transcript but as separate proteins (IRES vectors)

Monomeric proteins for fusion tags

DsRed-Monomer, AcGFP1, and mCherry proteins are truly monomeric fluorescent proteins that are ideal for use as fusion tags. mCherry is a very popular mutant derived by directed mutagenesis of mRFP1, a monomeric mutant of DsRed.

Bright multimers for reporter studies

ZsGreen1 and DsRed-Express are bright fluorescent proteins that are excellent reporters for gene expression or for measuring transduction/transfection efficiency. ZsGreen1, for example, is 2.5X brighter than EGFP.

Overview

  • Retroviral transduction for high-efficiency gene delivery to a broad range of cell types
  • Bright red and green fluorescent proteins for reporter studies
  • Bright monomeric red and green fluorescent proteins are ideal for fusion studies
  • High titers when combined with the Retro-X Universal Packaging System
  • Also, see our lentiviral and adenoviral options

More Information

Applications

  • Reporter studies
  • Monitoring subcellular localization
  • Monitoring transduction efficiency and gene expression levels

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.