MSCV retrovirus system
The MSCV Retroviral Expression System (murine stem cell virus) contains vectors that are optimized for introducing and expressing target genes in pluripotent cell lines, including murine or human hematopoietic, embryonic stem (ES), and embryonal carcinoma (EC) cells. They can also be used effectively with most mammalian cell lines. This highly efficient system is ideal for analyzing gene function in development, embryogenesis, or immune response, in both cell culture and transgenic assays.
The MSCV Retroviral Expression System (murine stem cell virus) contains vectors that are optimized for introducing and expressing target genes in pluripotent cell lines, including murine or human hematopoietic, embryonic stem (ES), and embryonal carcinoma (EC) cells. They can also be used effectively with most mammalian cell lines. This highly efficient system is ideal for analyzing gene function in development, embryogenesis, or immune response, in both cell culture and transgenic assays.
Designed for expression in hematopoietic cells and embryonic stem cells
The MSCV system contains three vectors: pMSCVneo, pMSCVhyg, and pMSCVpuro. These vectors contain a specially designed long terminal repeat (LTR) from the murine stem cell PCMV virus. This LTR differs from the MMLV LTR by several point mutations and a deletion that together enhance transcriptional activation and prevent transcriptional suppression in ES and EC cells. As a result, the PCMV LTR drives high-level, constitutive expression of the target gene in stem cells or other mammalian cell lines.
Packaging cell line included
The MSCV system includes the RetroPack PT67 Packaging Cell Line, which produces high-titer virus able to infect a broad range of mammalian host cells.
Overview
- Efficient long-term gene expression in ES and EC cells
- Choice of three selectable markers
- Complete retroviral system including a PT67 packaging cell line
More Information
Applications
- Retroviral transduction of a broad range of cell types including embryonic stem cells and embryonic carcinoma cells
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Lentiviral product selection guide
Takara Bio offers a broad range of research tools for gene delivery using lentiviral vectors, including a selection of vector systems, packaging mixes, and titration kits. Use our selection guide for a brief description of the products and links to further information.
Lentiviral products guide Learning centerTakara Bio USA, Inc.
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