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Retrovirus

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Retroviral vectors for constitutive gene expression

Retrovirus vectors for constitituve expression with CMV or LTR promoters

We provide a choice of gamma-retroviral vectors and retroviral vector sets. All of these retroviral vectors are derived from the Moloney Murine Leukemia Virus (MMLV). Retroviral vectors transduce a wide range of hard-to-transfect dividing cells. Lentiviral or adenoviral vectors are recommended for non-dividing cells.

We provide a choice of gamma-retroviral vectors and retroviral vector sets. All of these retroviral vectors are derived from the Moloney Murine Leukemia Virus (MMLV). Retroviral vectors transduce a wide range of hard-to-transfect dividing cells. Lentiviral or adenoviral vectors are recommended for non-dividing cells.

Q-series retroviral vectors and vector sets

Our Q-series of vectors contains a CMV/MSV hybrid promoter in the 5' LTR to drive high titers during the packaging step. They also contain a self-inactivating 3' LTR to reduce promoter interference and drive high expression of your transgene from the internal CMV promoter. Resistance to antibiotic selection is achieved through ribosome binding to an internal ribosome entry site (IRES).

The Retro-X Q Vector Set gives you a choice of Q-series vector backbones with alternative resistance markers for selection with G418 (pQCXIN), hygromycin (pQCXIH), or puromycin (pQCXIP).

pQCXIX does not contain a selection marker but does contain two multiple cloning sites, one on each side of an IRES, to allow simultaneous expression of two transgenes.

L-series retroviral vectors, vector sets, and systems

pLNCX2 and pLXSN are basic retroviral vectors that utilize wild-type LTRs from MMLV. These allow you to express your gene of interest from either a CMV promoter (pLNCX2) or the 5'-LTR promoter (pLXSN). Integration of these constructs into the genome of your target cell can be selected for by adding G418 to your culture medium.

The Retro-X System includes three L-series retroviral expression vectors, pLNCX2, pLXSN, and pLAPSN. The last of which is a control reporter vector made by adding the bacterial alkaline phosphatase gene to pLXSN. Our RetroPack PT67 packaging cell line is also included in this system.

The LRCX Retroviral Vector Set gives you a choice of L-series vector backbones with alternative resistance markers for selection with G418 (pLNCX2), hygromycin (pLHCX), or puromycin (pLPCX).

pRetro-Lib contains Sfi1A and Sfi1B restriction sites flanked by a stuffer fragment to enable simple cloning of a full-length cDNA library using our SMART cDNA Library Construction Kit.

pDON-AI-2 and pMEI-5 vector series

pDON-A1-2 vectors contain a human CMV promoter within the U3 region of the 5' LTR, whereas expression from pMEI-5 is from the LTR promoter. Because pMEI-5 contains an intron with high splicing ability, 2–8 fold higher gene expression level can be expected compared to the pDON-AI-2 series.

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Cat. # Product Size Price License Quantity Details
635002 pRetro-Lib Vector 20 ug USD $375.00

pRetro-Lib, derived from Moloney murine leukemia virus (MoMuLV), is designed for delivery and expression of retroviral libraries or genes. Upon transfection into a packaging cell line, pRetro-Lib can transiently express, or integrate and stably express, a transcript containing (psi, the extended viral packaging signal) and the gene of interest. The 5' viral LTR in this vector contains a viral promoter that controls expression of genes cloned into the MCS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Required Products

Cat. # Product Size Price License Quantity Details
639206 Advantage® 2 PCR Kit 100 Rxns USD $580.00

This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 100 PCR reactions of 50 μl each.

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631503 pLNCX2 Retroviral Vector 20 ug USD $440.00

pLNCX2 is designed for retroviral gene delivery and expression. Upon transfection into a packaging cell line, pLNCX2 can transiently express, or integrate and stably express, a transcript containing the Ψ viral packaging signal, a selectable marker, and the geneof interest, which is cloned into the MCS under the control of the immediate early promoter of human cytomegalovirus (PCMV). The 5' viral LTR drives expression of the neomycin resistance gene for antibiotic selection in eukaryotic cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631509 pLXSN Retroviral Vector 20 ug USD $440.00

pLXSN is designed for retroviral gene delivery and expression of gene of interest. It contains the retroviral packaging sequence (Ψ+) and so, when transfected into a cell line expressing the retroviral structural proteins (gag, pol, and env), leads to packaging of viral particles. The 5' viral LTR in this vector contains a viral promoter that controls expression of the gene of interest.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631511 LRCX Retroviral Vector Set Each USD $575.00

A system composed of three retroviral cloning vectors, as well as 5' and 3' LNCX sequencing/PCR primers. The system is suitable for use with the Retro-X system (Cat No. 631508). The pLNCX2, pLPCX, and pLHCX retroviral vectors contain a gene encoding resistance to neomycin, puromycin, or hygromycin, respectively. With these vectors, a gene of interest is expressed under control of the CMV promoter. The vectors are excellent tools for expression studies in primary cell lines, explants, or whole animals.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631514 pQCXIN Retroviral Vector 20 ug USD $456.00

The pQCXIN Retroviral Vector is a self-inactivating retroviral Q expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. It expresses a target gene along with the gene for the neomycin resistance marker from a single, bicistronic message. The CMV promoter present in the 5' LTR produces high viral titers in standard packaging cell lines. A deletion in the 3' LTR provides the self-inactivating feature after reverse transcription of the expression cassette. This vector is useful for applications in which high titers and high-level gene expression are desired.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631515 pQCXIX Retroviral Vector 20 ug USD $456.00

The pQCXIX Retroviral Vector is a self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. It expresses two target genes from a single, bicistronic message. The CMV promoter present in the 5' LTR produces high viral titers in standard packaging cell lines. A deletion in the 3' LTR provides the self-inactivating feature after reverse transcription of the expression cassette. This vector is useful for applications in which high titers and high-level bicistronic gene expression are desired.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631516 Retro-X™ Q Vector Set 4 x 20 ug USD $625.00

The Retro-X Q Vector set provides four retroviral expression vectors that are optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. Each vector expresses a target gene along with a different selection marker from a single, bicistronic message. The CMV promoter present in the 5' LTR produces high viral titers in standard packaging cell lines. A LacZ Control Vector pQCLIN is included in the set. Also included are Retro-X Q Primers that encode sequences flanking the multiple cloning site.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631501 pLXIN Retroviral Vector 20 ug USD $440.00

pLXIN is a bicistronic retroviral vector containing an internal ribosome entry site (IRES). The 5' LTR thus regulates expression of a gene of interest and the neomycin resistance gene on the same bicistronic message.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Selection guides Lentiviral vector options
Selection guides Retrovirus products
Concentrate retrovirus preps 100-fold without ultracentrifugation Retro-X Concentrator

Overview

  • Retroviral vectors designed for high retroviral titers and high expression of your transgene
  • Compatible with all Takara Bio brand retroviral packaging cell lines
  • Vector information is listed on our vector information pages
  • Fluorescent protein, MSCV, and tetracycline-inducible retroviral vectors are described elsewhere

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Choose the right viral system

Choose the right viral system

Use these tables to compare the characteristics of lentivirus, retrovirus, adenovirus, and adeno-associated virus (AAV), and select the best recombinant viral gene delivery system for your needs.

Compare viruses Product pages

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