632564
pLVX-tdTomato-C1 Vector
10 ug
USD $676.00
This lentiviral expression vector encodes the tdTomato fluorescent protein tag. This very bright red fluorescent protein is a genetic fusion of two copies of dTomato, which was specifically designed for low aggregation. It adopts an intramolecular tandem dimer structure that contributes to its exceptional brightness, yet it behaves like a monomer. Inserting a cDNA in the MCS downstream of the tdTomato coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells.
To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632563
pLVX-tdTomato-N1 Vector
10 ug
USD $676.00
This lentiviral expression vector encodes the tdTomato fluorescent protein tag. This very bright red fluorescent protein is a genetic fusion of two copies of dTomato, which was specifically designed for low aggregation. It adopts an intramolecular tandem dimer structure that contributes to its exceptional brightness, yet it behaves like a monomer. Inserting a cDNA in the MCS upstream of the tdTomato coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells.
To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632534
pCMV-tdTomato Vector
20 ug
USD $529.00
The mammalian CMV promoter in pCMV-tdTomato allows the constitutive expression of the tandem version of the dimeric red fluorescent protein, Tomato, driven by the constitutive immediate early promoter of cytomegalovirus (CMV). This vector does not contain an MCS. It can be used for labeling purposes or as a cotransfection marker to determine transfection efficiency.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632533
ptdTomato-C1 Vector
20 ug
USD $529.00
ptdTomato-C1 is a mammalian expression vector containing a tandem sequence of the dimeric red fluorescent protein, Tomato. An MCS at the 3' end of the encoding sequence makes it possible to clone in a gene of interest in order to express it as a C-terminal fusion protein with tdTomato. The unmodified vector can be used to express tdTomato in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632532
ptdTomato-N1 Vector
20 ug
USD $529.00
ptdTomato-N1 is a mammalian expression vector containing a tandem sequence of the dimeric red fluorescent protein, Tomato. An MCS at the 5' end of the coding sequence makes it possible to insert a gene of interest in order to express it as an N-terminal fusion with tdTomato. The unmodified vector can be used to express tdTomato in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632531
ptdTomato Vector
20 ug
USD $529.00
ptdTomato encodes a tandem sequence of the dimeric red fluorescent protein, Tomato. In this vector, the tdTomato coding sequence is flanked by unique restriction sites at the 5' and 3' ends, making it easy to excise the sequence for transfer into another vector. The ptdTomato vector contains a lac promoter sequence, and therefore can be used to express tdTomato in E. coli. It is primarily intended to serve as a source of tdTomato cDNA.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632190
DD-tdTomato Reporter System
Each
USD $569.00
The DD-tdTomato Reporter System includes the pDD-tdTomato Reporter vector and Shield1. The pDD-tdTomato Reporter is a promoterless vector that allows you to insert your promoter of interest upstream of the red fluorescent protein tdTomato, tagged at its N-terminus with the ProteoTuner destabilization domain (DD). The DD causes the reporter protein to be rapidly targeted to and degraded by proteasomes. This very efficient and controllable destabilization method will minimize background fluorescence from leaky promoters prior to promoter activation.
To analyze promoter activity, a candidate inducer is added to the medium along with Shield1, which effectively stabilizes the reporter protein and allows it to accumulate. As a result, only the reporter molecules expressed during promoter induction will contribute to the fluorescence signal, providing a considerably higher signal-to-noise ratio than that obtained with non-destabilized or constitutively destabilized reporter systems.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631975
pEF1alpha-tdTomato Vector
10 ug
USD $555.00
pEF1α-tdTomato is a mammalian expression vector that constitutively expresses the red fluorescent protein tdTomato, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of tdTomato is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows the protein to be expressed without the transgene silencing associated with CMV promoters. The vector, which lacks an MCS, is designed to be used for cell labeling or as a marker of transfection efficiency.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631753
Lenti-X™ DD Red Reporter System
Each
USD $1096.00
The Lenti-X DD Red Reporter System includes the Lenti-X DD-tdTomato Vector Set and Shield1. The Lenti-X DD-tdTomato Vector Set includes two lentiviral expression vectors (a reporter vector and a control vector) that produce high titers of recombinant lentivirus, which can efficiently transduce both dividing and nondividing mammalian cells. The system provides enough reagents for 16 packaging reactions. The pLVX-DD-tdTomato Reporter Vector is a promoterless vector that allows you to insert your promoter of interest upstream of the red fluorescent protein tdTomato, tagged at its N-terminus with the ProteoTuner destabilization domain (DD). In the absence of the membrane-permeant ligand Shield1, the DD causes the reporter protein to be rapidly targeted to and degraded by proteasomes. This very efficient and controllable destabilization method minimizes reporter background fluorescence from leaky promoters prior to promoter activation. To analyze promoter activity, a candidate inducer is added to the medium along with Shield1, which binds to the DD tag and thereby stabilizes the reporter protein and allows it to accumulate. As a result, only the reporter molecules expressed during promoter induction will contribute to the fluorescence signal, providing a considerably higher signal-to-noise ratio than that obtained with nondestabilized or constitutively destabilized reporter systems. Cells used to monitor uninduced promoters (e.g., the negative control) will be treated with Shield1 alone.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631238
pLVX-IRES-tdTomato Vector
20 ug
USD $676.00
The pLVX-IRES-tdTomato Vector is a bicistronic lentiviral expression vector that can be used to generate high-titer lentivirus for transducing dividing or nondividing mammalian cells. The vector contains an internal ribosomal entry site (IRES) which allows a gene-of-interest and the tdTomato fluorescent protein to be simultaneously coexpressed from a single mRNA transcript. When used with the Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line (Cat. No. 632180), the vector generates high titers of replication-incompetent, VSV-G-pseudotyped lentivirus.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
