AAV helper-free vector systems

Our helper-free AAV systems are designed for the preparation of high-titer AAV particles without the use of a helper virus. Each kit includes three plasmids encoding the factors necessary to prepare recombinant AAV particles by transfection of HEK293T cells. To prepare particles, co-transfect HEK293T cells with the three plasmids. Then, 2–3 days later extract particles using the included AAV Extraction Solution, a reagent that allows simple and high-yield AAV particle isolation while minimizing protein and nucleic acid contamination. In addition to offering AAV vectors for constitutive or inducible expression, we also provide AAVpro vectors for gene editing via CRISPR/Cas9 or Cre recombinase.