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Adeno-associated virus (AAV)

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AAV2 tetracycline-inducible expression system

Tet-One systems provide all necessary components for tetracycline-inducible (tet) expression on a single vector delivered by AAV2 virus. The Tet-On 3G transactivator is expressed from the human phosphoglycerate kinase 1 (PGK) promoter, and the cloned gene of interest is expressed from the P_TRE3GS promoter. The AAVpro system contains the reagents to generate the Tet-One vector, as well as components to efficiently package and isolate AAV2 particles.

There are also several other Tet-One vector options for mammalian cells.

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634310

AAVpro® Tet-One™ Inducible Expression System (AAV2)

1 System

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License Statement

ID Number
42	Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 8383364, # 9181556 , European patents EP # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems. For license information, please contact: GSF/CEO TET Systems GmbH & Co. KG, Im Neuenheimer Feld 582 69120 Heidelberg Germany Tel: +49 6221 5880400 Fax: +49 6221 5880404 email: info@tetsystems.com or use the electronic licensing request form via https://www.tetsystems.com/licensing/
M83	This product is the subject of the claims of US patent No. 9422576 and its foreign counterparts.
M84	This product is the subject of the claims of US patent No. 10072250 and its foreign counterparts.

The AAVpro Tet-One Inducible Expression System is a tetracycline-inducible gene expression system that allows you to produce high titers of recombinant, adeno-associated virus (AAV) for establishing a tightly inducible expression system for your gene of interest in mammalian cells. The all-in-one pAAV-TetOne vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter, and your gene of interest from the P_TRE3GS promoter. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene. There is no selection marker on this vector for mammalian cells. The kit also includes the plasmids for preparation of high-titer AAV particles without the use of a helper virus, and AAV Extraction Solution for isolation of pure AAV particles.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

Required Products

Cat. #	Product	Size	Price
631101	Tet System Approved FBS, US-Sourced	500 mL	USD $971.00
Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States. Documents Components Image Data Back Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels. Back Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS. Back 631101: Tet System Approved FBS, US-Sourced
631106	Tet System Approved FBS	500 mL	USD $933.00
Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. Documents Components Image Data Back Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels. Back Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS. Back 631106: Tet System Approved FBS

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

All-in-one vector design allows you to establish tet-inducible expression of your gene of interest with a single vector
Low background expression and high induction
Transactivator expression from the human PGK promoter allows inducible expression in a broad range of tissues
The unique AAV packaging system allows preparation of high-titer AAV2 without the use of a helper virus
Kit includes AAV Extraction Solution that increases viral particle recovery as compared to conventional freeze-thaw methods

More Information

Applications

Tightly controlled, tetracycline-inducible expression of your gene of interest in mammalian cells

References

Heinz, N. et al. Retroviral and transposon-based tet-regulated all-in-one vectors with reduced background expression and improved dynamic range. Hum. Gene Ther. 22, 166–176 (2011).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Tet-One tetracycline-inducible expression system, technology overview

Tighly controlled expression system that is as easy to use as any constitutive system

Until now, the best inducible mammalian expression systems have all required a two-vector format. Tet-One inducible expression systems are the only commercially available single-vector systems that can deliver a >10,000-fold difference in expression levels between induced and uninduced states.

Tet-One technology overview All inducible systems

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AAV2 tetracycline-inducible expression system

Notice to purchaser

Establishing an inducible expression system in target cells with AAVpro Tet-One

Transduction and induction in dividing cell lines with different MOIs of TetOne-Luc Control AAV

Induction of luciferase after transduction with TetOne-Luc Control AAV in the presence of different concentrations of Dox

Different induction times of luciferase after transduction with TetOne-Luc Control AAV

634310: AAVpro Tet-One Inducible Expression System (AAV2)

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

631101: Tet System Approved FBS, US-Sourced