AAV purification kits
The purity of AAV particles is an important factor for obtaining efficient and stable gene transfer in cultured cells and individual animals. However, many currently available AAV purification approaches, such as cesium chloride density gradient centrifugation and iodixanol ultracentrifugation, are time-consuming and require specialized equipment. Click "More" to see how our kits provide a solution.
The purity of AAV particles is an important factor for obtaining efficient and stable gene transfer in cultured cells and individual animals. However, many currently available AAV purification approaches, such as cesium chloride density gradient centrifugation and iodixanol ultracentrifugation, are time-consuming and require specialized equipment.
Our AAVpro Purification Kit Midi and Maxi (All Serotypes) (Cat. # 6675 and 6666, respectively) can be used to easily purify AAV particles from AAV-producing cell lysates through a simple precipitation and filtration protocol in about four hours.
Alternatively, AAVpro Cell & Sup. Purification Kit Maxi (All Serotypes) (Cat. # 6676) allows the researcher to purify virus particles from both AAV-producing cell lysates and culture supernatant in about seven hours. This provides higher virus yields, especially for serotypes that localize predominantly in supernatants or in both cell lysates and supernatants.
For the number of culture vessels per purification, please see the table below.
Overview
- Simple workflow: optimized protocol that eliminates the need for freeze-thaw cycles and ultracentrifugation steps
- High purity for reliable data: purified AAV is suitable for both in vitro and in vivo research
- Serotype agnostic: precipitation and filtration protocol that can purify AAV particles from any serotype
| Product | Cat. # | Extraction source | Serotype(s) | Size | # of flasks or dishes per purification |
|---|---|---|---|---|---|
| AAVpro Purification Kit Midi (All Serotypes) | 6675 | Cell lysate | All, but predominantly cell-associated serotypes | 4 purifications |
|
| AAVpro Purification Kit Maxi (All Serotypes) | 6666 | Cell lysate | All, but predominantly cell-associated serotypes | 4 purifications |
|
| AAVpro Cell & Sup. Purification Kit Maxi (All Serotypes) | 6676 | Cell lysate and culture supernatant | All | 4 purifications |
|
Please note that the purification method largely depends on the serotype, as different capsid types behave differently within producer cells. This affects where the virus accumulates and how it can be most effectively recovered. See the table below for examples of cellular localization of typical serotypes.
| AAV serotype | Predominant location | Key factor |
|---|---|---|
| AAV1 | Supernatant | Released efficiently into the culture medium |
| AAV2 | Cell pellet | Strong affinity for heparin sulfate causes retention within the cells, requiring cell lysis for harvest |
| AAV3 | Cell pellet | A low percentage of viral particles is found in the supernatant |
| AAV4 | Cell pellet | Only about 8% of particles are found in the supernatant |
| AAV5 | Supernatant | More readily released into the culture medium compared to AAV2 |
| AAV6 | Cell pellet | Primarily associated with cells, though some release occurs |
| AAV8 | Supernatant | Found in abundance in and can be efficiently harvested from the culture medium |
| AAV9 | Supernatant | Similar to AAV8, it is largely released into the supernatant |
More Information
References
Büning, H. and Srivastava, A. Capsid modifications for targeting and improving the efficacy of AAV vectors. Mol. Ther. Methods Clin. Dev. 12, 248–265 (2019).
Chahal, P.S. et al. Production of adeno-associated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery. J. Virol. Methods 196, 163–173 (2014).
Vandenberghe, L.H. et al. Efficient serotype-dependent release of functional vector into the culture medium during adeno-associated virus manufacturing. Human Gene Therapy 21 (2010).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Powered by Bioz
Fast and simple AAV purification
Need to purify AAV particles quickly or without ultracentrifugation? Watch these videos to learn about our AAVpro "All Serotypes" kit, a simple column-based purification method for easy purification of AAV particles from virus-producing cells.
- Unique AAV vector extraction method eliminates the need for ultracentrifugation
- Kits include all reagents needed to purify AAV particles from vector-producing cells
- Protocol yields highly pure AAV particles that can be used directly for in vivo transduction, or transduction of cultured cells
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2026 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
