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Home › Products › Gene function › Viral transduction › Adeno-associated virus (AAV) › qPCR titration

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AAV real-time PCR titration kit

Accurately deterimine titer of recombinant AAV using real time PCR

The AAVpro titration kit contains all of the reagents necessary for extraction of adeno-associated virus (AAV) particles from AAV-producing cells and real-time PCR analysis of the viral genomic sequence. This kit allows for more precise and rapid quantification of viral titer than conventional DNA blot and ELISA methods.

The AAVpro titration kit contains all of the reagents necessary for extraction of adeno-associated virus (AAV) particles from AAV-producing cells and real-time PCR analysis of the viral genomic sequence. This kit allows for more precise and rapid quantification of viral titer than conventional DNA blot and ELISA methods.

Regardless of the serotype (determined by the Cap gene used for packaging), the viral genome encapsulated within most commonly used recombinant AAV systems typically contain the ITRs (inverted terminal repeats) of AAV2. With this kit, amplification of the AAV2 ITRs is used to quantify titer and can, therefore, be used for determining the titer of any AAV serotype, as long as the ITRs are derived from AAV2. If you are unsure whether this kit will work for your vector, contact our technical services department with your vector sequence.

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Cat. # Product Size License Quantity Details
6233 AAVpro® Titration Kit (for Real Time PCR) Ver.2 100 Rxns USD $552.00

License Statement

ID Number  
M84 This product is the subject of the claims of US patent No. 10072250 and its foreign counterparts.
M85 This product is the subject of the claims of US patent No. 10161011 and its foreign counterparts.
M83 This product is the subject of the claims of US patent No. 9422576 and its foreign counterparts.

The AAVpro Titration Kit (for Real Time PCR) is a kit for determining the titer of adeno-associated virus (AAV) using real-time PCR. This kit contains all of the reagents necessary for extraction of AAV particles from AAV vector-producing cells and real-time PCR assay of virus genome. This kit allows more precise and rapid quantification than conventional DNA blotting or ELISA methods. Quantification is based on amplification of the ITRs (inverted terminal repeats) of AAV, and therefore titer measurement is possible for various serotypes of AAV.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Titer measurement of AAV by real-time PCR using ITR and CMV promoter targets

Titer measurement of AAV by real-time PCR using ITR and CMV promoter targets

Titer measurement of AAV by real-time PCR using ITR and CMV promoter targets. The viral genomic titers of AAV1, 2, and 6 particles were evaluated using two primer sets, one for the CMV region and one for the ITR region (AAVpro titration kit). For each serotype, there was no difference in the viral titer determined by real-time PCR amplification of either the CMV or ITR region. This data indicates that the AAVpro Titration kit can be used for titration of any serotypes that have genomic AAV2 ITRs.

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6233: AAVpro Titration Kit (for Real Time PCR) Ver.2

6233: AAVpro Titration Kit (for Real Time PCR) Ver.2

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Extraction of AAV particles and associated vector genomes

Extraction of AAV particles and associated vector genomes

Extraction of AAV particles and associated vector genomes. An overview of the process using our AAVpro Titration Kit (for Real Time PCR) Ver.2.

AAV purification kits AAV purification kit product info
Selection guides AAV workflow
Increase AAV yield by at least 3-fold with AAVpro Extraction Solution Extract 3X the virus

Overview

  • Can be used to determine the titer of AAV of any serotype as long as the ITRs are from AAV2 (most commonly used constructs)
  • Kit contains all reagents necessary for extraction of AAV particles from virus-producing cells and real-time PCR assay of AAV2 ITRs
  • Simple and fast titration method—results in approximately 2.5 hours
  • High-sensitivity titration—more precise quantification than conventional DNA blot and ELISA methods

More Information

Applications

  • Determining the titer of AAV using real-time PCR
  • Rapid and precise titration of any AAV serotype; quantification is based on the ITRs of AAV2, a sequence that is present in commonly used AAV vectors

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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Powered by Bioz See more details on Bioz

Determine titer of adeno-associated virus preps using qPCR

Consistent data requires consistent titer

Kit that combines qPCR with TB Green chemistry, allowing you to very accurately determine the viral genome copy number in AAV preparations from a calibrated DNA standard curve.

AAV qPCR titration kit Learning center

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