Adenovirus purification kits
Purify adenovirus quickly and easily with our adenovirus purification kits! These kits are chromatography-based systems that enable you to purify amplified adenovirus directly from the cell pellet in less than 1.5 hours. You simply pellet the cells when the cytopathic effect (CPE) is nearly complete, lyse the cells, and purify the virus.
Our Adeno-X Maxi Purification Kit has a wide range of viral capacities, allowing you to scale up or down without difficulty.
Purify adenovirus quickly and easily with our adenovirus purification kits! These kits are chromatography-based systems that enable you to purify amplified adenovirus directly from the cell pellet in less than 1.5 hours. You simply pellet the cells when the cytopathic effect (CPE) is nearly complete, lyse the cells, and purify the virus.
Our Adeno-X Maxi Purification Kit has a wide range of viral capacities, allowing you to scale up or down without difficulty. The kit delivers as many as 1 x 1012 adenoviral particles and provides all of the buffers and materials necessary for purification. This same technology is also available in our convenient Adeno-X Mega Purification Kits with which you can produce and purify up to 1013 highly infectious viral particles (up to 3 x 1011 IFU).
Unsure which adenovirus purification kit to use?
| How much lysate do I have? | Which kit do I need? |
| 5 x 15 cm plates | Adeno-X Maxi Purification Kit |
| 25 x 15 cm plates | Adeno-X Mega Purification Kit |
Simple chromatographic procedure
Our protocol contains no ultracentrifugation steps. Instead, adenovirus is purified chromatographically using a unique membrane that selectively binds adenoviral particles. The membrane is housed in a small, single-use cartridge that fits securely on standard size Luer-Lok syringes. For added convenience, the syringe-filter assembly comes completely preassembled and ready to use. The adenovirus-containing medium is brought into the syringe through tubing attached to a one-way valve and is pushed out through the purification cartridge. As virus-containing medium passes through the purification cartridge, the adenoviral particles adhere to the membrane and are effectively removed from the solution. The bound particles are then washed and eluted.
Achieve high titers of purified adenovirus
The high titer and purity achieved by our Adeno-X purification kits is comparable to that achieved by CsCl density gradient centrifugation, with viral particle-to-infectious unit ratios as low as 20:1. Unlike techniques involving CsCl, our method is safe, and requires no advanced training or expensive equipment—only simple manipulations are required to produce consistently high yields of purified adenovirus.
A powerful pump makes scaling up rapid and easy
To collect large amounts of virus use the Mega kit. With this kit we have adopted the use of a syringe pump (e.g., Genie Plus from Kent Scientific) to drive the clarified lysate through the three-tiered purification cartridge. The consistent pressure and flow rate provided by the pump during the binding, wash, and elution steps provides excellent virus recovery.
Overview
- Purify up to 1013 adenovirus particles in 90 min
- Use lysate from as many as 5 x 15 cm plates (maxi kit) or 25 x 15 cm plates (mega kit)
- Easy-to-use adenovirus purification assembly
- No CsCl or ultracentrifugation required
NOTE: For the mega kit, we recommend the use of a syringe pump (e.g., Genie Plus from Kent Scientific) to to drive the clarified lysate through the three-tiered purification cartridge.
More Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Choose the right viral system
Use these tables to compare the characteristics of lentivirus, retrovirus, adenovirus, and adeno-associated virus (AAV), and select the best recombinant viral gene delivery system for your needs.
Compare viruses Product pagesTakara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2020 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
