We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept

Home › Products › Gene function › ProteoTuner protein control systems › Plasmid systems

Gene function

Need help?
Contact Sales
Shield1 ligand for protein stabilization Shield1 ligand
Overviews Tet-One overview

ProteoTuner expression systems in plasmid format

Shield1-mediated control of protein expression

ProteoTuner plasmid expression systems allow for rapidly regulating the presence or absence of a protein of interest in your target cells. Clone your gene of interest in-frame with the destabilization domain tag (DD), either at the N or C terminus. In the absence of the Shield1 ligand in the culture medium, the DD-tagged fusion protein is immediately destabilized, but when Shield1 is added, the protein accumulates to detectable levels within 15–20 minutes.

ProteoTuner plasmid expression systems allow for rapidly regulating the presence or absence of a protein of interest in your target cells. Clone your gene of interest in-frame with the destabilization domain tag (DD), either at the N or C terminus. In the absence of the Shield1 ligand in the culture medium, the DD-tagged fusion protein is immediately destabilized, but when Shield1 is added, the protein accumulates to detectable levels within 15–20 minutes. Upon Shield1 removal, the halftime for the fusion protein's degradation can be as short as 30 minutes. Lentiviral and retroviral formats are also available.

 More  Less
Cat. # Product Size License Quantity Details
632189 Shield1 500 uL USD $360.00

The Shield1 compound stabilizes a protein of interest tagged with the destabilization domain (DD), which is based on a mutated version of the FKBP protein. It is used to protect the DD fusion protein of interest from proteasomal degradation. The amount of Shield1 can be varied to tune the amount of stabilized protein of interest within the cell.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
632188 Shield1 5 mg USD $820.00

After reconstitution in your solvent of choice, the Shield1 compound stabilizes a protein of interest tagged with the destabilization domain (DD), which is based on a mutated version of the FKBP protein. It is used to protect the DD fusion protein of interest from proteasomal degradation. The amount of Shield1 can be varied to tune the amount of the stabilized protein of interest in vivo.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
632172 ProteoTuner™ Shield System N Each USD $700.00

Direct manipulation of the presence and absence of a specific protein of interest is a very powerful tool for analyzing protein function. The ProteoTuner Shield System N uses a unique method to regulate the amount of protein of interest present in a cell, quickly and directly. It utilizes a ligand-dependent destabilization domain (DD) and its membrane permeable stabilizing ligand, Shield1. The DD is based on a 12 kDa mutant of the FKBP protein, which is expressed as a tag onto the N-terminus of your protein of interest cloned into the pPTuner Vector. In the presence of Shield1, the DD-tagged protein is stabilized and will accumulate inside the cell. This ligand-dependent stabilization occurs very quickly, and has been observed as soon as 15–30 minutes after the addition of Shield1. However, in the absence of the protective ligand Shield1, the DD-tagged protein of interest is rapidly degraded. Removing the Shield1 (by splitting the cells into media without Shield1) allows for protein destabilization, causing a fast degradation of the protein of interest. The extent of stabilization via Shield1 directly correlates with the amount of the ligand in the medium, so it is possible to tune the amount of protein of interest present in the cell, by controlling the amount of the Shield1 ligand.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources
632168 ProteoTuner™ Shield System N (w/ AcGFP1) Each USD $700.00

Direct manipulation of the presence and absence of a specific protein of interest is a very powerful tool for analyzing protein function. The ProteoTuner Shield System N (w/ AcGFP1) uses a unique method to regulate the amount of protein of interest present in a cell, quickly and directly. It utilizes a ligand-dependent destabilization domain (DD) and its membrane permeable stabilizing ligand, Shield1. The DD is based on a 12 kDa mutant of the FKBP protein, which is expressed as a tag onto the N-terminus of your protein of interest. In the presence of Shield1, the DD-tagged protein is stabilized and will accumulate inside the cell. This ligand-dependent stabilization occurs very quickly, and has been observed as soon as 15–30 minutes after the addition of Shield1. However, in the absence of the protective ligand Shield1, the DD-tagged protein of interest is rapidly degraded. Removing the Shield1 (by splitting the cells into media without Shield1) allows for protein destabilization, causing a fast degradation of the protein of interest. The process of stabilization via Shield1 is reversible, so it is possible to tune the amount of protein of interest present in the cell, by controlling the amount of the Shield1 ligand.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources
631072 ProteoTuner™ Shield System C Each USD $700.00

The ProteoTuner Shield System C utilizes a ligand-dependent destabilization domain (DD-C) and a membrane-permeant stabilizing ligand, Shield1, to quickly and directly regulate the amount of your protein of interest present in a cell or organism. First, your gene of interest is cloned into the pPTunerC Vector and expressed as a fusion with the destabilization domain (DD-C). Then the Shield1 ligand is either added or withheld, depending on your experiment.

In the presence of Shield1, the DD-C-tagged protein is stabilized and accumulates inside the cell. This ligand-dependent stabilization occurs very quickly, and has been observed as soon as 15–30 minutes after the addition of Shield1. In the absence of Shield1, the DD-C-tagged protein of interest is unstable. Consequently, removal of Shield1 allows for active and controllable degradation of the protein of interest. The extent of stabilization via Shield1 can be adjusted, and directly correlates with the amount of the ligand in the medium. Therefore, you can tune the level of your protein of interest present in the cell, by controlling the amount of the Shield1 ligand.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Learn more about our tet, ProteoTuner, and iDimerize inducible systems Inducible systems
Overviews ProteoTuner explained
Selection guides ProteoTuner kit selection guide

Overview

  • Rapid kinetics: protein level changes in minutes allows accurate functional analysis
  • Precise tuning: precise control of protein level by controlling the dose of Shield1
  • Reversible control: "protein on" to "protein off" for convincing gene-function studies
  • What you get: each kit is supplied with a plasmid vector and an aliquot of Shield1.
  • NOTE: Most of the proteins that we tested showed a better destabilization profile when the DD tag was fused to the N-terminus of the protein of interest (Systems N). Specific DD tag mutants for C-terminal tagging are available as well (System C); however they have a slightly reduced destabilization activity in the absence of the Shield1 ligand.

More Information

Applications

  • Protein function in pathways
  • Functional analysis of subunits of a protein complex
  • Functional analysis of essential proteins

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Control protein stability using a small molecule ligand, Shield1

Control protein stability with Shield1

ProteoTuner systems allow quick, predictable regulation of protein presence or absence in mammalian cells by acting directly on the protein, using a small, cell-permeable synthetic compound. When Shield1 is added to the medium, any fusion protein containing a DD domain can accumulate to detectable levels within 15–20 minutes. Conversely, upon Shield1 removal, the half-time for the protein's degradation can be as short as 30 minutes. This post-translational regulation offers many benefits, from speed and convenience to precise tuning and reversible control.

Technology explained Product selection guide

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

©2023 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top