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Fluorescent labeling and transfection control

Fluorescent labeling and transfection control

These vectors are ideally suited as controls to measure transfection efficiency. Simply co-transfect a red fluorescent protein vector with the target plasmid, and the resulting cells that are labeled with the fluorescent protein will also likely be transfected with the experimental plasmid. Each vector expresses a fluorescent protein from a constitutive promoter (CMV or EF1-alpha) and, if required, stable transfected cells can be selected for using G418.

These vectors are ideally suited as controls to measure transfection efficiency. Simply co-transfect a red fluorescent protein vector with the target plasmid, and the resulting cells that are labeled with the fluorescent protein will also likely be transfected with the experimental plasmid. Each vector expresses a fluorescent protein from a constitutive promoter (CMV or EF1-alpha) and, if required, stable transfected cells can be selected for using G418. Transfected cells can also be used in assays that require fluorescent labeling of whole cells.

These vectors do not contain a multiple cloning site; other fluorescent protein vectors are available for cloning applications. Click here for green fluorescent protein options.

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Cat. # Product Size Price License Quantity Details
632556 pCMV-E2-Crimson Vector 20 ug Inquire for Quotation *

The mammalian CMV promoter in pCMV-E2-Crimson allows the constitutive expression of the far-red Living Colors fluorescent protein E2-Crimson, driven by the constitutive immediate early promoter of cytomegalovirus (CMV). pCMV-E2-Crimson does not contain an MCS. It can be used for labeling purposes or as a cotransfection marker to determine transfection efficiency. If required, stable transfectants can be selected using G418.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632539 pCMV DsRed-Express2 Vector 20 ug Inquire for Quotation *

pCMV DsRed-Express2 is designed for whole cell labeling or to be used as a cotransfection marker in mammalian cells; the red fluorescence of DsRed-Express2 can be detected by fluorescence microscopy or flow cytometry, providing direct visual evidence of transfection or the ability to monitor cells after transplantation experiments in vivo. After cotransfection with pCMV DsRed-Express2 and an expression construct of interest, cells can also be sorted by flow cytometry (FACS) to enrich for transfected cells. pCMV DsRed-Express2 Vector can be transfected into mammalian cells using any standard transfection method. If required, stable transfectants can be selected using G418.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632534 pCMV-tdTomato Vector 20 ug USD $529.00

The mammalian CMV promoter in pCMV-tdTomato allows the constitutive expression of the tandem version of the dimeric red fluorescent protein, Tomato, driven by the constitutive immediate early promoter of cytomegalovirus (CMV). This vector does not contain an MCS. It can be used for labeling purposes or as a cotransfection marker to determine transfection efficiency.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632416 pCMV-DsRed-Express Vector 20 ug Inquire for Quotation *

pCMV-DsRed-Express is a mammalian expression vector designed for use as a cotransfection marker, together with an expression construct of interest. It constitutively expresses the red fluorescent protein DsRed-Express, which can be detected by fluorescence microscopy to provide direct visual evidence of transfection. Cells can also be sorted by flow cytometry to enrich for transfected cells.

pCMV-DsRed-Express Vector can be transfected into mammalian cells using any standard transfection method. If required, stable transfectants can be selected using G418.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631981 pEF1alpha-E2-Crimson Vector 10 ug Inquire for Quotation *

pEF1α-E2-Crimson is a mammalian expression vector that constitutively expresses the far-red fluorescent protein E2-Crimson, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of E2-Crimson is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows the protein to be expressed without the transgene silencing associated with CMV promoters. The vector, which lacks an MCS, is designed to be used for cell labeling or as a marker of transfection efficiency.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631979 pEF1alpha-DsRed-Express2 Vector 10 ug Inquire for Quotation *

pEF1α-DsRed-Express2 is a mammalian expression vector that constitutively expresses the red fluorescent protein DsRed-Express2, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of DsRed-Express2 is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows the protein to be expressed without the transgene silencing associated with CMV promoters. The vector, which lacks an MCS, is designed to be used for cell labeling or as a marker of transfection efficiency.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631975 pEF1alpha-tdTomato Vector 10 ug Inquire for Quotation *

pEF1α-tdTomato is a mammalian expression vector that constitutively expresses the red fluorescent protein tdTomato, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of tdTomato is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows the protein to be expressed without the transgene silencing associated with CMV promoters. The vector, which lacks an MCS, is designed to be used for cell labeling or as a marker of transfection efficiency.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

  • Bright fluorescent proteins for labeling transfected cells
  • Choice of CMV or EF1-alpha promoter
    • CMV promoter for the strongest transcriptional activity in the broadest range of cell types
    • Human elongation factor-1 alpha (EF1-alpha) promoter that can be used in cells where CMV can be silenced (e.g., embryonic stem cells and some hematopoietic cells)
  • Multiple red color options
    • tdTomato is the brightest of the red fluorescent proteins and is also extremely suitable for in vivo imaging applications
    • E2-Crimson, a far-red fluorescent protein, is bright, very soluble, and matures extremely fast (half time of 26 minutes at 37°C). It exhibits low cytotoxicity and is well-suited for in vivo applications involving sensitive cells such as primary cells and stem cells.

More Information

Applications

  • Fluorescent reporter for transient and stable transfections
  • Whole-cell labeling

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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