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Fluorescent labeling and transfection control

These vectors are ideally suited as controls to measure transfection efficiency. Simply co-transfect a red fluorescent protein vector with the target plasmid, and the resulting cells that are labeled with the fluorescent protein will also likely be transfected with the experimental plasmid. Each vector expresses a fluorescent protein from a constitutive promoter (CMV or EF1-alpha) and, if required, stable transfected cells can be selected for using G418.

These vectors do not contain a multiple cloning site; other fluorescent protein vectors are available for cloning applications. Click here for green fluorescent protein options.

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Cat. #

Product

Size

License

Quantity

Details

632556

pCMV-E2-Crimson Vector

20 ug

USD $589.00

License Statement

ID Number

The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.

Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pCMV-E2-Crimson Vector product License Agreement can be found by clicking here.

The mammalian CMV promoter in pCMV-E2-Crimson allows the constitutive expression of the far-red Living Colors fluorescent protein E2-Crimson, driven by the constitutive immediate early promoter of cytomegalovirus (CMV). pCMV-E2-Crimson does not contain an MCS. It can be used for labeling purposes or as a cotransfection marker to determine transfection efficiency. If required, stable transfectants can be selected using G418.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

632539

pCMV DsRed-Express2 Vector

20 ug

USD $571.00

License Statement

ID Number

Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pCMV DsRed-Express2 Vector product License Agreement can be found by clicking here.

The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.

pCMV DsRed-Express2 is designed for whole cell labeling or to be used as a cotransfection marker in mammalian cells; the red fluorescence of DsRed-Express2 can be detected by fluorescence microscopy or flow cytometry, providing direct visual evidence of transfection or the ability to monitor cells after transplantation experiments in vivo. After cotransfection with pCMV DsRed-Express2 and an expression construct of interest, cells can also be sorted by flow cytometry (FACS) to enrich for transfected cells. pCMV DsRed-Express2 Vector can be transfected into mammalian cells using any standard transfection method. If required, stable transfectants can be selected using G418.

Notice to purchaser

Components

632534

pCMV-tdTomato Vector

20 ug

USD $529.00

License Statement

ID Number
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pCMV-tdTomato Vector product License Agreement can be found by clicking here.
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.

The mammalian CMV promoter in pCMV-tdTomato allows the constitutive expression of the tandem version of the dimeric red fluorescent protein, Tomato, driven by the constitutive immediate early promoter of cytomegalovirus (CMV). This vector does not contain an MCS. It can be used for labeling purposes or as a cotransfection marker to determine transfection efficiency.

Notice to purchaser

Components

632416

pCMV-DsRed-Express Vector

20 ug

USD $589.00

License Statement

ID Number

Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pCMV-DsRed-Express Vector product License Agreement can be found by clicking here.

The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.

pCMV-DsRed-Express is a mammalian expression vector designed for use as a cotransfection marker, together with an expression construct of interest. It constitutively expresses the red fluorescent protein DsRed-Express, which can be detected by fluorescence microscopy to provide direct visual evidence of transfection. Cells can also be sorted by flow cytometry to enrich for transfected cells.

pCMV-DsRed-Express Vector can be transfected into mammalian cells using any standard transfection method. If required, stable transfectants can be selected using G418.

Notice to purchaser

Components

631981

pEF1alpha-E2-Crimson Vector

10 ug

USD $555.00

License Statement

ID Number

The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.

Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pEF1alpha-E2-Crimson Vector product License Agreement can be found by clicking here.

pEF1α-E2-Crimson is a mammalian expression vector that constitutively expresses the far-red fluorescent protein E2-Crimson, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of E2-Crimson is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows the protein to be expressed without the transgene silencing associated with CMV promoters. The vector, which lacks an MCS, is designed to be used for cell labeling or as a marker of transfection efficiency.

Notice to purchaser

Components

631979

pEF1alpha-DsRed-Express2 Vector

10 ug

USD $555.00

License Statement

ID Number

The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.

Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pEF1alpha-DsRed-Express2 Vector product License Agreement can be found by clicking here.

pEF1α-DsRed-Express2 is a mammalian expression vector that constitutively expresses the red fluorescent protein DsRed-Express2, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of DsRed-Express2 is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows the protein to be expressed without the transgene silencing associated with CMV promoters. The vector, which lacks an MCS, is designed to be used for cell labeling or as a marker of transfection efficiency.

Notice to purchaser

Components

631975

pEF1alpha-tdTomato Vector

10 ug

USD $555.00

License Statement

ID Number
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pEF1alpha-tdTomato Vector product License Agreement can be found by clicking here.

pEF1α-tdTomato is a mammalian expression vector that constitutively expresses the red fluorescent protein tdTomato, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of tdTomato is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows the protein to be expressed without the transgene silencing associated with CMV promoters. The vector, which lacks an MCS, is designed to be used for cell labeling or as a marker of transfection efficiency.

Notice to purchaser

Components

Overview

Bright fluorescent proteins for labeling transfected cells
Choice of CMV or EF1-alpha promoter
- CMV promoter for the strongest transcriptional activity in the broadest range of cell types
- Human elongation factor-1 alpha (EF1-alpha) promoter that can be used in cells where CMV can be silenced (e.g., embryonic stem cells and some hematopoietic cells)

Multiple red color options
- tdTomato is the brightest of the red fluorescent proteins and is also extremely suitable for in vivo imaging applications
- E2-Crimson, a far-red fluorescent protein, is bright, very soluble, and matures extremely fast (half time of 26 minutes at 37°C). It exhibits low cytotoxicity and is well-suited for in vivo applications involving sensitive cells such as primary cells and stem cells.

More Information

Applications

Fluorescent reporter for transient and stable transfections
Whole-cell labeling

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Fluorescent labeling and transfection control

Notice to purchaser

Notice to purchaser

Robust expression of DsRed-Express2 in mouse bone marrow hematopoietic stem and progenitor cells

Notice to purchaser

tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model

632534: pCMV-tdTomato Vector

Notice to purchaser

Notice to purchaser

Map schematic of the plasmid choices that are available carrying the EF1-alpha promoter

Notice to purchaser

Robust expression of DsRed-Express2 in mouse bone marrow hematopoietic stem and progenitor cells

Map schematic of the plasmid choices that are available carrying the EF1-alpha promoter

631979: pEF1alpha-DsRed-Express2 Vector

Notice to purchaser

Map schematic of the plasmid choices that are available carrying the EF1-alpha promoter

631975: pEF1alpha-tdTomato Vector

Overview

More Information

Applications

Additional product information