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  • EpiXplore Methylated DNA Enrichment Kit
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  • EpiScope Nucleosome Preparation Kit
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Home › Products › Gene function › Cell biology assays › Epigenetics › DNA methylation › EpiXplore Methylated DNA Enrichment Kit

Epigenetics

  • Detecting DNA and chromatin modifications
    • Methylation-dependent restriction enzyme
  • DNA methylation
    • EpiXplore Methylated DNA Enrichment Kit
    • EpiScope MSP Kit
    • EpiScope Nucleosome Preparation Kit
    • EpiTaq HS
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EpiXplore Methylated DNA Enrichment Kit

The EpiXplore Methylated DNA Enrichment Kit provides a simple magnetic bead-based method for enriching and fractionating both hypo- and hypermethylated DNA from the whole genome. Enrichment leads to superior results in gene regulation studies by reducing background during sequencing.

The EpiXplore Methylated DNA Enrichment Kit provides a simple magnetic bead-based method for enriching and fractionating both hypo- and hypermethylated DNA from the whole genome. Enrichment leads to superior results in gene regulation studies by reducing background during sequencing.

Why choose the EpiXplore Methylated DNA Enrichment Kit?

  • The EpiXplore protocol is based on affinity binding between methylated DNA and Methylated DNA Binding Domain Protein 2 (MBD2).
  • It does not require any antibodies or follow an immunoprecipitation (IP) protocol.
  • Its flexible protocol allows you to elute your methylated DNA in fractions (by methylation density) or as a single fraction.

By contrast, antibody-based (IP) enrichment methods, including MeDIP, lack flexibility and the necessary pretreatments often result in loss of starting material. More importantly, antibody-based enrichment is “all or none”—that is, it does not allow gradient separation of methylated DNA.

Why fractionate by methylation density?

Recent reports indicate that regions of the genome with medium to low density methylation (including regions far from promoters and CpG islands) can play an important role in differential gene regulation. If you are looking for a comprehensive picture of methylation activity, differentiating these regions is an important first step. Only the EpiXplore Methylated DNA Enrichment Kit includes elution buffers which allow you to split your enriched methylated DNA into three fractions, based on methylation density.

How many methylated sites are required for binding?

The EpiXplore Methylated DNA Enrichment Kit contains a fragment of the MBD2 protein which provides high sensitivity. The kit can enrich DNA fragments containing as few as three methylated CpG pairs (6 total methylation sites).

Can your current enrichment method compete?

Is your enriched methylated DNA…EpiXplore protocol Antibody-based protocols
HTS-ready? YES. Enriched methylated DNA is double-stranded and ready for use No. Enriched DNA requires further processing
Intact? YES. Uses a gentle (magnetic) separation process No. Harsh separation conditions often lead to sample loss
Easy to elute? YES. Uses a simple buffer-based protocol No. Elution requires multiple complex steps
Separated by methylation density? YES. Methylated DNA can be eluted in low, medium, and high density fractions or as a single fraction No. Elution is “all or none”
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Cat. # Product Size License Quantity Details
631964 Magnetic Stand 2 x 1.5 mL USD $56.00

Magnetic stand for 1.5 ml tubes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Image Data

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Methylated DNA enrichment protocol overview

Methylated DNA enrichment protocol overview
Methylated DNA enrichment protocol overview.

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Highly sensitive methylated DNA enrichment

Highly sensitive methylated DNA enrichment
Highly sensitive methylated DNA enrichment. The kit enriches DNA fragments containing ≥ 3 methylated CpG pairs (6 total methylation sites). FT: Flow-through containing unbound (non- or hypomethlyated) DNA. W: Wash. EL: Elution fraction containing methylated DNA.

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631964: Magnetic Stand

631964: Magnetic Stand
631963 EpiXplore™ Methylated DNA Enrichment Kit 10 Rxns USD $249.00

The EpiXplore Methylated DNA Enrichment Kit provides a simple magnetic bead-based method for enriching and fractionating hypo- and hypermethylated DNA fragments from the whole genome. Enrichment leads to superior results in downstream applications that enhance the broader understanding of gene regulation, such as mapping methylation sites, by reducing background during sequencing. This is the 10 rxn version of this kit. For the 20 rxn version see Cat. # 631962.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Methylated DNA enrichment protocol overview

Methylated DNA enrichment protocol overview
Methylated DNA enrichment protocol overview.

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Magnetic Stand for 1.5-ml microcentrifuge tubes

Magnetic Stand for 1.5-ml microcentrifuge tubes

Magnetic Stand for 1.5-ml microcentrifuge tubes (Cat. # 631964). Use with magnetic beads for protein purification, immunoprecipitation, co-immunoprecipitation, and cell-capture studies.

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Highly sensitive methylated DNA enrichment

Highly sensitive methylated DNA enrichment
Highly sensitive methylated DNA enrichment. The kit enriches DNA fragments containing ≥ 3 methylated CpG pairs (6 total methylation sites). FT: Flow-through containing unbound (non- or hypomethlyated) DNA. W: Wash. EL: Elution fraction containing methylated DNA.

Back

631963: EpiXplore Methylated DNA Enrichment Kit

631963: EpiXplore Methylated DNA Enrichment Kit

Required Products

Cat. # Product Size Price License Quantity Details
631964 Magnetic Stand 2 x 1.5 mL USD $56.00

Magnetic stand for 1.5 ml tubes.

Image Data

Back

Methylated DNA enrichment protocol overview

Methylated DNA enrichment protocol overview
Methylated DNA enrichment protocol overview.

Back

Highly sensitive methylated DNA enrichment

Highly sensitive methylated DNA enrichment
Highly sensitive methylated DNA enrichment. The kit enriches DNA fragments containing ≥ 3 methylated CpG pairs (6 total methylation sites). FT: Flow-through containing unbound (non- or hypomethlyated) DNA. W: Wash. EL: Elution fraction containing methylated DNA.

Back

631964: Magnetic Stand

631964: Magnetic Stand
631962 EpiXplore™ Methylated DNA Enrichment Kit 20 Rxns USD $440.00

The EpiXplore Methylated DNA Enrichment Kit provides a simple magnetic bead-based method for enriching and fractionating hypo- and hyper-methylated DNA fragments from the whole genome. Enrichment of methylated DNA leads to reduced background during sequencing, providing superior results in applications used to understand epigenetic gene regulation, such as DNA methylation profiling. This is the 20 rxn version of this kit. For the 10 rxn version see Cat. # 631963.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Methylated DNA enrichment protocol overview

Methylated DNA enrichment protocol overview
Methylated DNA enrichment protocol overview.

Back

Magnetic Stand for 1.5-ml microcentrifuge tubes

Magnetic Stand for 1.5-ml microcentrifuge tubes

Magnetic Stand for 1.5-ml microcentrifuge tubes (Cat. # 631964). Use with magnetic beads for protein purification, immunoprecipitation, co-immunoprecipitation, and cell-capture studies.

Back

Highly sensitive methylated DNA enrichment

Highly sensitive methylated DNA enrichment
Highly sensitive methylated DNA enrichment. The kit enriches DNA fragments containing ≥ 3 methylated CpG pairs (6 total methylation sites). FT: Flow-through containing unbound (non- or hypomethlyated) DNA. W: Wash. EL: Elution fraction containing methylated DNA.

Back

631962: EpiXplore Methylated DNA Enrichment Kit

631962: EpiXplore Methylated DNA Enrichment Kit

Required Products

Cat. # Product Size Price License Quantity Details
631964 Magnetic Stand 2 x 1.5 mL USD $56.00

Magnetic stand for 1.5 ml tubes.

Image Data

Back

Methylated DNA enrichment protocol overview

Methylated DNA enrichment protocol overview
Methylated DNA enrichment protocol overview.

Back

Highly sensitive methylated DNA enrichment

Highly sensitive methylated DNA enrichment
Highly sensitive methylated DNA enrichment. The kit enriches DNA fragments containing ≥ 3 methylated CpG pairs (6 total methylation sites). FT: Flow-through containing unbound (non- or hypomethlyated) DNA. W: Wash. EL: Elution fraction containing methylated DNA.

Back

631964: Magnetic Stand

631964: Magnetic Stand
5324 Magnosphere™ MS300/Carboxyl 4 mL USD $1064.00

Magnosphere MS300/Carboxyl is a magnetic particle developed for high purity bioseparation. It has excellent performance as a carrier for enzyme immunoassays, immunoprecipitation/Western blot analysis, DNA probe immobilization, etc. Magnosphere MS300/Carboxyl has a uniform particle size and shows superparamagnetism, making it ideal for use in magnetic separation and redispersion operations.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents

Overview

  • A simple magnetic bead-based method for enriching methylated DNA from the whole genome
  • Retain >95% of methylated DNA
  • Enriched DNA is immediately ready for downstream sequencing
  • Protocol allows for gradient separation of low, medium, and highly methylated DNA

More Information

Applications

  • Mapping methylation sites
  • Methylation-sensitive restriction enzyme-based assays
  • Real time and endpoint PCR assays
  • Bisulfite sequencing
  • Library preparation for high-throughput sequencing
  • Sample prep for DNA microarray analysis

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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