LymphoONE T-Cell Expansion Xeno-Free Medium
LymphoONE T-Cell Expansion Xeno-Free Medium is specifically formulated for expansion and transduction of human T lymphocytes. It has been optimized to enable efficient T-cell proliferation with or without the addition of human serum or plasma. LymphoONE medium contains no protein other than pharmaceutical-grade human serum albumin and recombinant human insulin, and provides superior performance relative to other commonly used xeno-free media. A phenol red-free GMP-grade version is also available.
LymphoONE T-Cell Expansion Xeno-Free Medium is specifically formulated for expansion and transduction of human T lymphocytes. It has been optimized to enable efficient T-cell proliferation with or without the addition of human serum or plasma. LymphoONE medium contains no proteins other than pharmaceutical-grade human serum albumin and recombinant human insulin, and provides superior performance relative to other commonly used xeno-free media. A phenol red-free GMP-grade version is also available.
Conventional culture systems typically require the addition of serum (or plasma) in order to achieve sufficient quantities of T cells for research applications, but the availability of autologous serum varies, and it has been demonstrated that T cells expanded in the absence of serum can offer improved performance (Janetzki et al. 2010; Medvec et al. 2018). By enabling robust expansion in the absence of human serum or plasma, LymphoONE medium is well-suited for immunotherapy research involving T cells.
T-cell activation using LymphoONE T-Cell Expansion Xeno-Free Medium in combination with anti-CD3 antibody, IL-2, and RetroNectin reagent results in enhanced T-cell expansion and yields higher proportions of naïve T cells relative to approaches employing IL-2 in combination with anti-CD3 and anti-CD28 antibodies.
As demonstrated by the data below, expansion in LymphoONE medium also yields higher proportions of naïve T cells as compared to other commonly used media, with or without the addition of serum.
Phenotypic analysis of T cells expanded using various culture media in the presence or absence of serum. Peripheral blood mononuclear cells (PBMCs) were cultured for four days under stimulation by anti-CD3 monoclonal antibody, IL-2, and RetroNectin reagent in CultiLife 215 Culture Bags with or without addition of human serum using LymphoONE T-Cell Expansion Xeno-Free Medium, X-VIVO 15 (Lonza), or AIM V (Thermo Fisher Scientific). The cells were then cultured at appropriate dilutions with or without serum for an additional six days in each of the respective media supplemented with IL-2. Human serum was added at a concentration of 1% from Day 0 to Day 7, and then at a concentration of 0.5% from Day 7 to Day 10. Expansion in LymphoONE medium resulted in higher proportions of cells expressing markers associated with a naïve T-cell phenotype (CD45RA+/CCR7+), with or without addition of serum.
Overview
- Xeno-free medium that enables efficient T-cell expansion with or without the addition of serum or plasma
- Supplemented with human serum albumin, recombinant human insulin, L-glutamine, and streptomycin
- Endotoxin tested (endotoxin <0.3 EU/ml)
- Compatible with CultiLife culture bags for large-scale expansion of T cells
- Anti-CD3/IL-2 T-cell activation in the presence of RetroNectin reagent* enhances proliferation and leads to a higher proportion of naïve T cells
- Available as GMP grade medium for developing cell therapy applications
*T-cell expansion by RetroNectin stimulation for uses other than research requires a license from Takara Bio Inc.
More Information
Applications
- Expansion of T cells
- Gene transfer by lentiviral or retroviral transduction
- Enhance culture and expansion of T cells and CAR-T cells
Reference
Janetzki, S. et al. Performance of serum-supplemented and serum-free media in IFNgamma Elispot Assays for human T cells. Cancer Immunol. Immunother. 59, 609–18 (2010).
Medvec, A. R. et al. Improved expansion and in vivo function of patient T cells by a serum-free medium. Mol. Ther. Methods Clin. Dev. 8, 65–74 (2018).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Application data and FAQs for LymphoONE medium
An overview of features and benefits and FAQs about LymphoONE T-Cell Expansion Xeno-Free Medium.
T-cell expansion
RetroNectin Recombinant Human Fibronectin Fragment and RetroNectin GMP grade have widespread use as enhancers of retroviral or lentiviral gene transfer into hard-to-transduce cell types. Here, we present data on an additional application—the improved expansion of T cells from PBMCs and increased production of naïve T cells—using a combination of our products: GT-T551 T-cell medium, RetroNectin reagent, CultiLife culture bags, and anti-CD3 antibody.
T-cell expansion tech note Related products
Scale up transduction of hematopoietic cells with CultiLife culture bags
CultiLife culture bags provide a closed, sterile, gas-permeable system that reduces the risk of culture contamination and user exposure to cell culture materials. A 300-ml format (which can be coated with RetroNectin reagent) is available for high-efficiency viral transduction of hematopoietic cells and a 1,000-ml format can be used to expand cell cultures following transduction. View our protocol and see how to set up your cell culture in a single small bag and then expand it into multiple larger bags.
View protocol and specifications Buy nowTakara Bio USA, Inc.
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