Cell signaling pathway profiling systems

Our pathway profiling systems provide a means to assess cell signaling pathway activation in vivo. These systems consist of sets of vectors that each contain a distinct cis-acting enhancer element upstream of a reporter gene. A single transfection experiment provides a profile of the transcription factors that are affected by a given stimulus, drug candidate, or gene product of interest.

Profile multiple signal transduction pathways

Most of the cis-acting enhancer elements included in our transcription factor systems are regulated by more than one transcription factor so that multiple signal transduction pathways can be monitored. Reporter expression indicates that a transactivator present in the cell has bound the enhancer element in one of the TransFactor vectors, indicating that a particular signaling pathway or pathways has been activated.

Two reporters available—luciferase & SEAP

• Luciferase induction can be measured from cell lysates using a standard assay.
• SEAP can be measured simply by collecting samples of the culture medium without cell lysis. Takara Bio offers the Great EscAPe SEAP Chemiluminescence Kit 2.0 to measure SEAP activity.