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  • ‹ Back to Reporter systems
  • Fluorescent protein promoter reporters
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  • Secreted alkaline phosphatase assays
  • Beta galactosidase and LacZ vectors
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  • Fucci cell-cycle vectors
  • Lenti-X Actin Dynamics Monitoring Kit
Home › Products › Gene function › Cell biology assays › Reporter systems › Fucci cell-cycle vectors

Cell biology assays

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Fucci cell-cycle vectors

Our cell-cycle reporter vectors allow for real-time monitoring of cell-cycle progression in living cells without fixation. They even let you visualize cell shape.

Our cell-cycle reporter vectors allow for real-time monitoring of cell-cycle progression in living cells without fixation. They even let you visualize cell shape.

Fucci probes

Cell-cycle reporter vectors deliver fluorescent, ubiquitination-based cell-cycle indicators (Fucci) that allow you to identify cells in various phases of the cell cycle. These Fucci probes contain Cdt1 or Geminin, proteins whose levels fluctuate differentially throughout the cell cycle: Cdt1 levels peak in G1 phase; as cells transition into S phase, Cdt1 levels fall and Geminin levels rise, remaining high until the cells are back in G1. Cells control Cdt1 and Geminin levels post-translationally, using ubiquitination to target the unwanted proteins for proteasomal degradation. To allow Geminin and Cdt1 to be easily visualized, each is expressed with a red or cyan Living Colors fluorescent tag. These probes allow precise, visual evaluation of the cell-cycle phase.

Monitor cell cycle and cell shape in real time

Our Fucci cell-cycle reporters let you label just the nucleus, or both the nucleus and cytoplasm, allowing visualization of cell shape. Although most Fucci probes label only cell nuclei, we offer a truncated version of hGeminin that is able to migrate to the cytoplasm between S and M phases, enabling visualization of cell morphology.

A variety of Fucci probes and delivery options

We offer retrovirus and plasmid-based vectors for the delivery of a variety of Fucci probes. Each probe consists of a red or cyan fluorescent tag, and a deletion mutant of either hCdt1 or hGeminin. Our retroviral vectors can either be transfected as plasmids or transduced as retrovirus in a wide variety of mammalian cells. Once integrated into the host genome, these vectors provide stable, heritable expression of the probes.

The plasmid-based vector, pTRE-CellCycle, allows tightly controlled, tetracycline (Tet)-inducible expression of two Fucci probes from a bidirectional promoter that can be induced using any Tet-On or Tet-Off technology. Expression of these probes causes cell nuclei to turn red during G1 phase and cyan during S, G2, and M phases, allowing complete visual tracking of the cell cycle.

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Cat. # Product Size License Quantity Details
631466 pTRE-CellCycle Vector 10 ug USD $597.00

License Statement

ID Number  
88 This product is sold under license from RIKEN and Tokyo Metropolitan Organization for Medical Research. This product is the subject of U.S. Patent No. 8,182,987 and its foreign counterparts.
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pTRE-CellCycle Vector product License Agreement can be found by clicking here.
42 Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 8383364, # 9181556 , European patents EP # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
GSF/CEO
TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
69120 Heidelberg
Germany
Tel: +49 6221 5880400
Fax: +49 6221 5880404
email: info@tetsystems.com
or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research

pTRE-CellCycle is a bidirectional, tetracycline (Tet)-inducible expression vector that lets you inducibly express fluorescent, ubiquitination-based, cell-cycle indicators (Fucci) in order to monitor all phases of the cell cycle. The vector allows you to identify cells that are either in G1 phase or transitioning between S, G2, and M phases. pTRE-CellCycle is intended for use with any Tet-On or Tet-Off Expression System.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Cell-cycle progression as visualized with Fucci probes

Cell-cycle progression as visualized with Fucci probes

Cell-cycle progression as visualized with Fucci probes. Successive rounds of division in HEK 293 Tet-On Advanced cells were monitored using Fucci probes expressed from the pTRE-CellCycle vector in the presence of doxycycline (Dox). As the cells progressed through the cell cycle, their nuclei varied in color: cells lacking color were transitioning between phases M and G1. The nuclei of cells in G1 phase were then labeled with the red fluorescent protein mCherry. As the cells transitioned between phases G1 and S, they appeared light pink/violet. Finally, the nuclei of cells in S through M phases were labeled with the cyan fluorescent protein AmCyan1.

Back

Retroviral delivery is available for a variety of cell-cycle Fucci probes

Retroviral delivery is available for a variety of cell-cycle Fucci probes

Retroviral delivery is available for a variety of cell-cycle Fucci probes. Fucci probes containing Geminin-fluorescent protein (fp) fusions are visible in phases S through M, whereas the probe containing Cdt1-fp (expressed by pRetroX-G1-Red) is visible during the G1 phase of the cell cycle. pRetroX-SG2Mcyto-Red expresses a truncated version of geminin that allows the cell shape to be visualized from phases S through M.

Back

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle. pTRE-CellCycle is a bidirectional, tetracycline (Tet)-inducible expression vector that lets you inducibly express Fucci probes during all phases of the cell cycle (Panel A). In cells transfected with the vector, the Cdt1 fusion is visible through G1 phase, while the Geminin fusion is visible from S through M phases (Panel B). A fluorescence micrograph of HEK 293 Tet-On Advanced cells transfected with the pTRE-CellCycle vector and cultured in the presence of Dox is shown in Panel C.

Back

Movie of cell-cycle progression, visualized with Fucci probes

Movie of cell-cycle progression, visualized with Fucci probes

Movie of cell-cycle progression, visualized with Fucci probes. No color: cells transitioning between phases M and G1. Red: cells in G1 phase. Light pink/violet: cells transitioning between phases G1 and S. Cyan: the nuclei of cells in phases S through M.

Back

Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle

Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle
Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle. Each probe consists of a red or cyan fluorescent tag, and a deletion mutant of either hCdt1 or hGeminin.

Back

High-efficiency transfection of seven of the most commonly used cell lines with Xfect Transfection Reagent

High-efficiency transfection of seven of the most commonly used cell lines with Xfect Transfection Reagent
High-efficiency transfection of seven of the most commonly used cell lines with Xfect Transfection Reagent.

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Customer testimonial: transfection of primary rat cardiomyocytes with Xfect Transfection Reagent

Customer testimonial: transfection of primary rat cardiomyocytes with Xfect Transfection Reagent
Customer testimonial: transfection of primary rat cardiomyocytes with Xfect Transfection Reagent.

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Our customers have indicated via survey that they have successfully used Xfect Transfection Reagent to transfect the cell lines listed here

Our customers have indicated via survey that they have successfully used Xfect Transfection Reagent to transfect the cell lines listed here
Our customers have indicated via survey that they have successfully used Xfect Transfection Reagent to transfect the cell lines listed here.

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631466: pTRE-CellCycle Vector

631466: pTRE-CellCycle Vector
631465 pRetroX-SG2M-Red Vector 10 ug USD $597.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pRetroX-SG2M-Red Vector product License Agreement can be found by clicking here.
88 This product is sold under license from RIKEN and Tokyo Metropolitan Organization for Medical Research. This product is the subject of U.S. Patent No. 8,182,987 and its foreign counterparts.

pRetroX-SG2M-Red is a self-inactivating retroviral vector that expresses mCherry-hGeminin (1–110), a fluorescent, ubiquitination-based, cell-cycle indicator (Fucci). The vector allows you to identify cells that are transitioning between S, G2, and M phases.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Cell-cycle progression as visualized with Fucci probes

Cell-cycle progression as visualized with Fucci probes

Cell-cycle progression as visualized with Fucci probes. Successive rounds of division in HEK 293 Tet-On Advanced cells were monitored using Fucci probes expressed from the pTRE-CellCycle vector in the presence of doxycycline (Dox). As the cells progressed through the cell cycle, their nuclei varied in color: cells lacking color were transitioning between phases M and G1. The nuclei of cells in G1 phase were then labeled with the red fluorescent protein mCherry. As the cells transitioned between phases G1 and S, they appeared light pink/violet. Finally, the nuclei of cells in S through M phases were labeled with the cyan fluorescent protein AmCyan1.

Back

Retroviral delivery is available for a variety of cell-cycle Fucci probes

Retroviral delivery is available for a variety of cell-cycle Fucci probes

Retroviral delivery is available for a variety of cell-cycle Fucci probes. Fucci probes containing Geminin-fluorescent protein (fp) fusions are visible in phases S through M, whereas the probe containing Cdt1-fp (expressed by pRetroX-G1-Red) is visible during the G1 phase of the cell cycle. pRetroX-SG2Mcyto-Red expresses a truncated version of geminin that allows the cell shape to be visualized from phases S through M.

Back

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle. pTRE-CellCycle is a bidirectional, tetracycline (Tet)-inducible expression vector that lets you inducibly express Fucci probes during all phases of the cell cycle (Panel A). In cells transfected with the vector, the Cdt1 fusion is visible through G1 phase, while the Geminin fusion is visible from S through M phases (Panel B). A fluorescence micrograph of HEK 293 Tet-On Advanced cells transfected with the pTRE-CellCycle vector and cultured in the presence of Dox is shown in Panel C.

Back

Movie of cell-cycle progression, visualized with Fucci probes

Movie of cell-cycle progression, visualized with Fucci probes

Movie of cell-cycle progression, visualized with Fucci probes. No color: cells transitioning between phases M and G1. Red: cells in G1 phase. Light pink/violet: cells transitioning between phases G1 and S. Cyan: the nuclei of cells in phases S through M.

Back

Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle

Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle
Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle. Each probe consists of a red or cyan fluorescent tag, and a deletion mutant of either hCdt1 or hGeminin.
631464 pRetroX-SG2Mcyto-Red Vector 10 ug USD $597.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pRetroX-SG2Mcyto-Red Vector product License Agreement can be found by clicking here.
88 This product is sold under license from RIKEN and Tokyo Metropolitan Organization for Medical Research. This product is the subject of U.S. Patent No. 8,182,987 and its foreign counterparts.

pRetroX-SG2Mcyto-Red is a self-inactivating retroviral vector that expresses mCherry-hGeminin (1–60), a fluorescent, ubiquitination-based, cell-cycle indicator (Fucci). The vector allows you to identify cells that are transitioning between S, G2, and M phases.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Cell-cycle progression as visualized with Fucci probes

Cell-cycle progression as visualized with Fucci probes

Cell-cycle progression as visualized with Fucci probes. Successive rounds of division in HEK 293 Tet-On Advanced cells were monitored using Fucci probes expressed from the pTRE-CellCycle vector in the presence of doxycycline (Dox). As the cells progressed through the cell cycle, their nuclei varied in color: cells lacking color were transitioning between phases M and G1. The nuclei of cells in G1 phase were then labeled with the red fluorescent protein mCherry. As the cells transitioned between phases G1 and S, they appeared light pink/violet. Finally, the nuclei of cells in S through M phases were labeled with the cyan fluorescent protein AmCyan1.

Back

Retroviral delivery is available for a variety of cell-cycle Fucci probes

Retroviral delivery is available for a variety of cell-cycle Fucci probes

Retroviral delivery is available for a variety of cell-cycle Fucci probes. Fucci probes containing Geminin-fluorescent protein (fp) fusions are visible in phases S through M, whereas the probe containing Cdt1-fp (expressed by pRetroX-G1-Red) is visible during the G1 phase of the cell cycle. pRetroX-SG2Mcyto-Red expresses a truncated version of geminin that allows the cell shape to be visualized from phases S through M.

Back

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle. pTRE-CellCycle is a bidirectional, tetracycline (Tet)-inducible expression vector that lets you inducibly express Fucci probes during all phases of the cell cycle (Panel A). In cells transfected with the vector, the Cdt1 fusion is visible through G1 phase, while the Geminin fusion is visible from S through M phases (Panel B). A fluorescence micrograph of HEK 293 Tet-On Advanced cells transfected with the pTRE-CellCycle vector and cultured in the presence of Dox is shown in Panel C.

Back

Movie of cell-cycle progression, visualized with Fucci probes

Movie of cell-cycle progression, visualized with Fucci probes

Movie of cell-cycle progression, visualized with Fucci probes. No color: cells transitioning between phases M and G1. Red: cells in G1 phase. Light pink/violet: cells transitioning between phases G1 and S. Cyan: the nuclei of cells in phases S through M.

Back

Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle

Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle
Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle. Each probe consists of a red or cyan fluorescent tag, and a deletion mutant of either hCdt1 or hGeminin.
631463 pRetroX-G1-Red Vector 10 ug USD $597.00

License Statement

ID Number  
88 This product is sold under license from RIKEN and Tokyo Metropolitan Organization for Medical Research. This product is the subject of U.S. Patent No. 8,182,987 and its foreign counterparts.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the pRetroX-G1-Red Vector product License Agreement can be found by clicking here.
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.

pRetroX-G1-Red is a self-inactivating retroviral vector that expresses mCherry-hCdt1 (30–120), a fluorescent, ubiquitination-based, cell-cycle indicator (Fucci). The vector allows you to identify cells that are in G1 phase.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Cell-cycle progression as visualized with Fucci probes

Cell-cycle progression as visualized with Fucci probes

Cell-cycle progression as visualized with Fucci probes. Successive rounds of division in HEK 293 Tet-On Advanced cells were monitored using Fucci probes expressed from the pTRE-CellCycle vector in the presence of doxycycline (Dox). As the cells progressed through the cell cycle, their nuclei varied in color: cells lacking color were transitioning between phases M and G1. The nuclei of cells in G1 phase were then labeled with the red fluorescent protein mCherry. As the cells transitioned between phases G1 and S, they appeared light pink/violet. Finally, the nuclei of cells in S through M phases were labeled with the cyan fluorescent protein AmCyan1.

Back

Retroviral delivery is available for a variety of cell-cycle Fucci probes

Retroviral delivery is available for a variety of cell-cycle Fucci probes

Retroviral delivery is available for a variety of cell-cycle Fucci probes. Fucci probes containing Geminin-fluorescent protein (fp) fusions are visible in phases S through M, whereas the probe containing Cdt1-fp (expressed by pRetroX-G1-Red) is visible during the G1 phase of the cell cycle. pRetroX-SG2Mcyto-Red expresses a truncated version of geminin that allows the cell shape to be visualized from phases S through M.

Back

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle. pTRE-CellCycle is a bidirectional, tetracycline (Tet)-inducible expression vector that lets you inducibly express Fucci probes during all phases of the cell cycle (Panel A). In cells transfected with the vector, the Cdt1 fusion is visible through G1 phase, while the Geminin fusion is visible from S through M phases (Panel B). A fluorescence micrograph of HEK 293 Tet-On Advanced cells transfected with the pTRE-CellCycle vector and cultured in the presence of Dox is shown in Panel C.

Back

Movie of cell-cycle progression, visualized with Fucci probes

Movie of cell-cycle progression, visualized with Fucci probes

Movie of cell-cycle progression, visualized with Fucci probes. No color: cells transitioning between phases M and G1. Red: cells in G1 phase. Light pink/violet: cells transitioning between phases G1 and S. Cyan: the nuclei of cells in phases S through M.

Back

Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle

Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle
Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle. Each probe consists of a red or cyan fluorescent tag, and a deletion mutant of either hCdt1 or hGeminin.

Back

631463: pRetroX-G1-Red Vector

631463: pRetroX-G1-Red Vector
631462 pRetroX-SG2M-Cyan Vector 10 ug USD $597.00

License Statement

ID Number  
88 This product is sold under license from RIKEN and Tokyo Metropolitan Organization for Medical Research. This product is the subject of U.S. Patent No. 8,182,987 and its foreign counterparts.

pRetroX-SG2M-Cyan is a self-inactivating retroviral vector that expresses AmCyan1-hGeminin (1–110), a fluorescent, ubiquitination-based, cell-cycle indicator (Fucci). The vector allows you to identify cells that are transitioning between S, G2, and M phases.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

Cell-cycle progression as visualized with Fucci probes

Cell-cycle progression as visualized with Fucci probes

Cell-cycle progression as visualized with Fucci probes. Successive rounds of division in HEK 293 Tet-On Advanced cells were monitored using Fucci probes expressed from the pTRE-CellCycle vector in the presence of doxycycline (Dox). As the cells progressed through the cell cycle, their nuclei varied in color: cells lacking color were transitioning between phases M and G1. The nuclei of cells in G1 phase were then labeled with the red fluorescent protein mCherry. As the cells transitioned between phases G1 and S, they appeared light pink/violet. Finally, the nuclei of cells in S through M phases were labeled with the cyan fluorescent protein AmCyan1.

Back

Retroviral delivery is available for a variety of cell-cycle Fucci probes

Retroviral delivery is available for a variety of cell-cycle Fucci probes

Retroviral delivery is available for a variety of cell-cycle Fucci probes. Fucci probes containing Geminin-fluorescent protein (fp) fusions are visible in phases S through M, whereas the probe containing Cdt1-fp (expressed by pRetroX-G1-Red) is visible during the G1 phase of the cell cycle. pRetroX-SG2Mcyto-Red expresses a truncated version of geminin that allows the cell shape to be visualized from phases S through M.

Back

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle

Tightly controlled, simultaneous expression of two Fucci probes allows complete visual tracking of the cell cycle. pTRE-CellCycle is a bidirectional, tetracycline (Tet)-inducible expression vector that lets you inducibly express Fucci probes during all phases of the cell cycle (Panel A). In cells transfected with the vector, the Cdt1 fusion is visible through G1 phase, while the Geminin fusion is visible from S through M phases (Panel B). A fluorescence micrograph of HEK 293 Tet-On Advanced cells transfected with the pTRE-CellCycle vector and cultured in the presence of Dox is shown in Panel C.

Back

Movie of cell-cycle progression, visualized with Fucci probes

Movie of cell-cycle progression, visualized with Fucci probes

Movie of cell-cycle progression, visualized with Fucci probes. No color: cells transitioning between phases M and G1. Red: cells in G1 phase. Light pink/violet: cells transitioning between phases G1 and S. Cyan: the nuclei of cells in phases S through M.

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Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle

Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle
Retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes to monitor the cell cycle. Each probe consists of a red or cyan fluorescent tag, and a deletion mutant of either hCdt1 or hGeminin.

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631462: pRetroX-SG2M-Cyan Vector

631462: pRetroX-SG2M-Cyan Vector

Overview

  • Retroviral vectors to track cell-cycle phase in living cells
  • Differentiate G1 phase from S/G2/M based on color
  • See the complete morphology of the cell during simultaneous monitoring of cell-cycle phase

More Information

Applications

  • Monitor cell-cycle transitions from G1 through G2/S/M phase
  • Visualize cell morphology during cell-cycle transition

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
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  • CRISPR screening
  • Drug discovery
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  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
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  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
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  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
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  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
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