Tet-Off cell lines for tetracycline-inducible expression
Tet-Off cell lines are host cell lines that greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by removing doxycycline (Dox) from the culture medium. Target cells that express the Tet-Off Advanced transactivator and also contain an integrated TRE-based expression vector (e.g., pTRE-Tight) will express high levels of your gene of interest (GOI) when cultured in the absence of the Dox.
Tet-On 3G cell lines, which are our highest-performing pre-made cell lines for this system, are located on a separate page.
Overview
- Stably expressed Tet-Off transactivator protein in commonly used cell lines
- Induce expression of your GOI by removing Dox from the culture of transfected cells
- Easily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g.,PTRE3G), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones
More Information
Applications
- Inducible gene expression in mammalian cells
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2026 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
