Application data and FAQs for LymphoONE medium
The development of therapies utilizing adoptive T-cell transfer has provided powerful new approaches for combating previously intractable diseases such as cancer. However, the broader application of these approaches will require additional technological innovation and refinement of existing protocols for culturing and engineering T cells. For example, in order to obtain sufficient quantities of patient-derived T cells for therapeutic applications, conventional culture systems typically require supplementation of culture medium with serum or plasma, which can be problematic for a variety of reasons:
- Supplies of autologous serum that can be obtained from a patient are limited and vary depending on the patient's condition
- Supplementation with donor serum of bovine or human origin is costly, introduces a source of unwanted variability in protocol performance, and raises the risk of pathogen exposure
- Culturing in the absence of serum yields T cells that exhibit improved downstream performance (Janetzki et al. 2010; Medvec et al. 2018)
Efficient T-cell expansion without serum
To better facilitate research involving T cell-based therapies, Takara Bio has developed LymphoONE T-Cell Expansion Xeno-Free Medium, a chemically defined medium that enables efficient T-cell expansion without the addition of serum or plasma. As demonstrated by the data below, LymphoONE medium enables greater expansion relative to Takara Bio's previous offering (GT-T551) and commonly used serum-free media from other suppliers (X-VIVO 15, AIM V).
Higher proportions of naïve T cells
In addition to expansion efficiency, another critical factor in the development of T cell-based therapies involves the phenotypic characteristics of expanded populations. Research over the past decade has established that T cells with a naïve phenotype provide greater therapeutic efficacy as compared to more differentiated populations (Klebanoff et al. 2015), an understanding that has informed the development of LymphoONE medium, as demonstrated below.
Janetzki, S. et al. Performance of serum-supplemented and serum-free media in IFNgamma Elispot Assays for human T cells. Cancer Immunol. Immunother. 59, 609–18 (2010).
Klebanoff et al. Memory T cell-driven differentiation of naive cells impairs adoptive immunotherapy. J. Clin. Invest. 126, 318–334 (2016).
Medvec, A. R. et al. Improved expansion and in vivo function of patient T cells by a serum-free medium. Mol. Ther. Methods Clin. Dev. 8, 65–74 (2018).
FAQs about LymphoONE T-Cell Expansion Xeno-Free Medium
How does LymphoONE medium perform with the addition of serum?
While LymphoONE medium was developed to enable efficient T-cell expansion in the absence of serum, researchers always have the option of supplementing the medium with serum or plasma in order to achieve greater proliferation. As demonstrated below by the data involving stimulation by IL-2, anti-CD3 mAb, and RetroNectin reagent, supplementation of LymphoONE medium with human serum results in greater T-cell expansion and yields higher proportions of naïve T cells (CD45RA+/CCR7+ phenotype) compared to serum-free conditions. The data also demonstrate that LymphoONE medium outperforms competitor media under both scenarios (with or without addition of serum).
Does LymphoONE medium contain antibiotics?
Yes, the version of LymphoONE medium that is currently available (Cat. #s WK552S & WK552) contains streptomycin. In the future, we will launch a GMP-grade version of the medium without antibiotics.
Does LymphoONE medium contain any animal-derived components?
No. LymphoONE medium is xeno-free, meaning that it does not include any animal-derived (nonhuman) components.
Does LymphoONE medium contain any human-derived components?
Yes. LymphoONE medium contains highly purified pharmaceutical-grade human serum albumin.
What other components does LymphoONE medium contain?
In addition to streptomycin and human serum albumin, LymphoONE medium also includes L-glutamine and recombinant human insulin.
Is LymphoONE medium chemically defined?
Yes. The identities and quantities of all medium components are known.
How does LymphoONE medium compare to Takara Bio's previous T-cell medium, GT-T551?
The composition of LymphoONE T-Cell Expansion Xeno-Free Medium is similar to that of its predecessor, GT-T551. However, the formulation of LymphoONE medium has been optimized to enable greater T-cell proliferation, as demonstrated by the data presented above.
Will there be a GMP-grade version of LymphoONE medium?
Yes. We currently plan to launch a GMP-grade version of LymphoONE medium towards the end of 2019.
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