We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us

Close

  • ‹ Back to iDimerize systems
  • Inducible protein-protein interactions—iDimerize systems
  • iDimerize systems journal club
  • iDimerize in vivo protocol
  • iDimerize systems citations
  • ARGENT cell signaling regulation kits from ARIAD
iDimerize inducible protein interaction iDimerize inducible protein interaction systems
Home › Learning centers › Gene function › Inducible systems › iDimerize systems › iDimerize systems journal club

Inducible systems

  • iDimerize systems
    • Inducible protein-protein interactions—iDimerize systems
    • iDimerize systems journal club
    • iDimerize in vivo protocol
    • iDimerize systems citations
    • ARGENT cell signaling regulation kits from ARIAD
  • ProteoTuner systems
    • ProteoTuner technology overview
    • ProteoTuner citations
    • Inducible protein stabilization systems product selection guide
  • Tet-inducible systems
    • Tet systems product selection guide
    • Tet systems overview
    • Tet-One technology overview
    • Tet-On 3G plasmid system kit components
    • Tet-On 3G lentiviral system kit components
    • Tet system webinars
New products
Need help?
Contact Sales
iDimerize inducible protein interaction iDimerize inducible protein interaction systems

iDimerize systems journal club

iDimerize journals

Put a switch on it...

Imagine activating your specific signaling pathway of interest on demand, independently of upstream signals! iDimerize Systems are powerful technologies for creating conditional alleles that can be activated within seconds of adding a small-molecule ligand.

Of the many publications using iDimerize technology, we have selected a few to highlight. Click each study for a summary.

Publications using iDimerize technology

Induced dimerization and activation of FGFR in mouse prostate Dimerization and activation of FGFR in mouse prostate

Research summary

iDimerize inducible homodimerization technology is used to dissect the roles of mammalian fibroblast growth factor receptor (FGFR) sub-class members in causing prostate hyperplasia in mice. The authors show that induced FGFR1 signaling, but not FGFR2 signaling, leads to prostate hyperplasia. 

Paper: Freeman, K. W. et al. Inducible prostate intraepithelial neoplasia with reversible hyperplasia in conditional FGFR1-expressing mice. Cancer Res. 63, 8256-63 (2003).


Ligand-induced dimerization & activation of FGFR in mouse prostate

Advantages of inducible dimerization

  • Activate one specific FGFR
  • Induce at any developmental time point
  • Remove ligand to study phenotype reversibility
  • Easier setup than cre-lox or regulated transcription systems

Experimental design

Mice with inducible FGFR1 and FGFR2 transgenes under the control of a prostate-specific promoter were created. Each construct encoded a membrane-targeting sequence, the tyrosine kinase domain of FGFR, and two copies of the DmrB domain, which dimerize in the presence of the B/B Homodimerizer ligand.*

*Studies are presented using iDimerize components. For complete experimental details, please refer to the original publication. The optimal position and copy number of fusion domains varies per study and must be determined empirically.

Key results

  1. After two weeks of regular injections with the ligand, only mice with ectopic FGFR1 signaling developed prostate-specific hyperplasia. Receptor tyrosine phosphorylation was confirmed in both stains, suggesting phenotypic differences were due to signaling events downstream of FGFR activation.
  2. Authors stopped ligand treatment at various time points to see if continued FGFR1 signaling is required for hyperplasia maintenance. They learned that hyperplasia is reversible prior to extensive neovascularization, providing opportunities for early intervention in prostate cancer.

Induced recruitment and activation of mammalian Rho GTPase Recruitment and activation of mammalian Rho GTPase

Research summary

iDimerize inducible heterodimerization technology is used to understand the role of Rho GTPase Cdc42 in regulating actin polymerization. Authors show that induced recruitment of Cdc42 to the membrane is sufficient for producing actin-based membrane protrusions. 

Paper: Castellano, F., Montcourrier, P. & Chavrier, P. Membrane recruitment of Rac1 triggers phagocytosis. J. Cell Sci. 2955–61 (2000).



Ligand-induced dimerization & activation of Cdc42 in mammalian cells

Advantages of inducible dimerization

  • Activate one specific GTPase pathway
  • Avoid cross-talk between related pathways
  • Eliminate the need for upstream stimuli
  • Isolate and study transient signaling events

Experimental design

An inducible system for recruiting Cdc42 to the plasma membrane was created in rat basophilic leukemia (RBL-2H3) cells. Two copies of the DmrA domain were anchored to the cell membrane using a signal sequence. The cytosolic, constitutively active form of Cdc42 was expressed fused to a DmrC domain, allowing inducible translocation to the plasma membrane upon addition of A/C Heterodimerizer ligand to growth media.*

*Studies are presented using iDimerize components. For complete experimental details, please refer to the original publication. The optimal position and copy number of fusion domains varies per study and must be determined experimentally.

Key results

  1. Localized recruitment of Cdc42 to the membrane was sufficient for producing the signature phenotype of actin-based membrane protrusions.
  2. A parallel study showed that membrane recruitment of Rac1 triggers actin-dependent particle internalization via phagocytosis (Castellano, F. 2000).

Induced FasR activation and macrophage apoptosis in mice FasR activation and macrophage apoptosis in mice

Research summary

iDimerize inducible homodimerization technology is used to rewire the Fas-dependent apoptosis pathway, and generate transgenic mice capable of conditional macrophage ablation. Authors show that induced Fas receptor (FasR) oligomerization leads to systemic macrophage apoptosis. 

Paper: Burnett, S. H. Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene. J. Leukoc. Biol. 75, 612-623 (2004).


Ligand-induced Fas receptor activation and macrophage apoptosis in mice

Advantages of inducible dimerization

  • Induction is systemic and reversible
  • Induce at any developmental stage
  • Remove ligand to study phenotype reversibility

Experimental design

Mafia (Macrophage Fas-induced apoptosis) mice with transgenes under the control of a macrophage-specific promoter were created. The cytoplasmic region of FasR, which mediates DISC assembly, was fused with two copies of the DmrB domain and a membrane-targeting domain. FasR oligomerization was induced by intraperitoneal injections with B/B Homodimerizer ligand.*

*Studies are presented using iDimerize components. For complete experimental details, please refer to the original publication. The optimal position and copy number of fusion domains varies per study and must be determined experimentally.

Key result

  1. Mafia mice receiving injections of ligand showed 70–95% loss of macrophages cells following the 5th day of treatment.

Induced caspase activation and fat cell apoptosis in mice Caspase activation and fat-cell apoptosis in mice

Research summary

iDimerize inducible homodimerization technology is used to rewire the apoptosis pathway and generate transgenic mice capable of conditional ablation of fat cells. Authors show that induced caspase-8 dimerization leads to systemic fat apoptosis. 

Paper: Pajvani, U. B. et al. Fat apoptosis through targeted activation of caspase 8: a new mouse model of inducible and reversible lipoatrophy. Nat. Med. 11, 797-803 (2005).


Ligand-induced Fas receptor activation and macrophage apoptosis in mice

Advantages of inducible dimerization

  • Induction is systemic and reversible
  • Lacks toxicity of constitutive models
  • Induce at any developmental stage
  • Remove ligand to study phenotype reversibility

Experimental design

FAT-ATTAC (fat apoptosis through targeted activation of caspase-8) mice with transgenes under the control of adipocytes-specific promoter were created. The catalytic domain of human caspase-8 was fused with a DmrB domain and a membrane-targeting domain. Caspase-8 dimerization was induced by intraperitoneal injections with B/B Homodimerizer ligand.*

*Studies are presented using iDimerize components. For complete experimental details, please refer to the original publication. The optimal position and copy number of fusion domains varies per study and must be determined empirically.

Key results

  1. FAT-ATTAC mice receiving injections of ligand showed a loss of white and brown fat, plus a marked drop in the adipocyte-specific markers adiponectin and resistin, within 7 days of treatment.
  2. Mice lost 12% of their total mean body weight by the 10th week of treatment.
  3. Phenotype recovered to a normal state within 2 months of stopping treatment.

Induced PIP2-mediated ion channel gating at the plasma membrane PIP2-mediated ion channel gating at the plasma membrane

Research summary

iDimerize inducible heterodimerization technology is used to understand the role of phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis by phospholipase C (PLC), in mediating KCNQ ion channel closure. The authors show that induced PIP2 depletion is sufficient for turning off KCNQ channels, without requiring other PLC-associated signaling molecules. 

Paper: Suh, B.-C., Leal, K. & Hille, B. Modulation of high-voltage activated Ca(2+) channels by membrane phosphatidylinositol 4,5-bisphosphate. Neuron 67, 224-38 (2010).  
     

Ligandi-nduced PIP2-Mediated Ion Channel Gating at the Plasma Membrane

Advantages of inducible dimerization

  • Allows targeted enzyme recruitment
  • Fast induction kinetics (seconds)
  • Remove ligand to study phenotype reversibility

Experimental design

Human embryonic kidney tsA-201 cells were created that deplete PIP2 using inositol polyphosphate 5-phosphatase (Inp54p), thus avoiding other signaling molecules generated downstream of PLC. Yeast Inp54p was fused with a DmrA domain and cyan fluorescent protein (CFP). A DmrC domain was anchored at the membrane using a signaling sequence, to allow inducible Inp54p recruitment upon addition of A/C Heterodimerizer ligand to the growth media. A yellow fluorescent protein (YFP)-labeled pleckstrin homology (PH) domain, which naturally binds membrane phosphatidylinositol lipids, was used as a sensor for PIP2 levels.*

*Studies are presented using iDimerize components. For complete experimental details, please refer to the original publication. The optimal position and copy number of fusion domains varies per study and must be determined empirically.

Key results

  1. In the presence of ligand, Inp54p accumulation at the membrane and a simultaneous release of the fluorescent sensor were observed, confirming PIP2 depletion at the membrane.
  2. Ligand treatment also caused complete and reversible suppression of KCNQ currents, proving that PIP2 depletion suffices in turning off KCNQ channels.

Induced insulin secretion and glucose regulation in diabetic mice Insulin secretion and glucose regulation in diabetic mice

Research summary

iDimerize inducible reverse dimerization technology is used to create a conditional export system for the controlled secretion of insulin through the endoplasmic reticulum (ER). Authors show rapid and reversible regulation of both insulin and glucose in hyperglycemic mice. 

Paper: Rollins, C. T. et al. A ligand-reversible dimerization system for controlling protein-protein interactions. Proc. Natl. Acad. Sci. U. S. A. 97, 7096-101 (2000).


Ligand-Induced Insulin Secretion and Glucose Regulation in Diabetic Mice

Advantages of inducible dimerization

  • Fast (in seconds) and reversible induction
  • Plasma-stable ligand inducer
  • Uses endogenous export machinery
  • Eliminates the need for upstream stimuli

Experimental design

Engineered cells carrying a transgene for inducible insulin secretion were transplanted in hyperglycemic mice. Transgenes expressed human proinsulin fused to four copies of the DmrD domain (two copies are shown in the figure). A furin cleavage sequence was included to allow removal of fusion domains in the trans-Golgi, and release of biologically active protein. In the absence D/D Solubilizer ligand, the DmrD domain self-aggregates, resulting in cargo that is too large for ER export. Ligand treatment solubilizes the aggregates, enabling export.*

*Studies are presented using iDimerize components. For complete experimental details, please refer to the original publication. The optimal position and copy number of fusion domains varies per study and must be determined empirically.

Key results

  1. Cells treated with ligand showed insulin release in a dose-dependent manner.
  2. Insulin secretion was observed 30 min following treatment and was fully terminated an hour after ceasing treatment.
  3. Hyperglycemic mice exhibited rapid regulation of both insulin and glucose levels 15 min following ligand administration.

Induced apoptosis of microglial cells to study retinal degeneration Induced apoptosis of microglial cells to study retinal degeneration

Research summary

Microglia have been reported to play a central role in chronic degenerative diseases. The iDimerize Inducible Homodimer System was used to specifically deplete bone marrow (BM)-derived microglia cells. This allowed researchers to specifically define the roles of retinal microglia and BM-derived microglia in a mouse model of retinal degeneration. 

Paper: Wang, N.-K. et al. Origin of fundus hyperautofluorescent spots and their role in retinal degeneration in a mouse model of Goldmann-Favre syndrome. Dis. Model. Mech. 6, 1113-22 (2013).


Ligand induced apoptosis of microglia in mice

Advantages of inducible dimerization

  • Induce apoptosis specifically at the appropriate developmental stage
  • Induction is systematic
  • Easier setup than other inducible systems
  • Homodimerizer does not cross the blood-brain barrier; apoptosis is induced in a specific subset of cells

Experimental design

Transgenic rd7 mice (a model for retinal degeneration) were created that contained the transgene for macrophage Fas-induced apoptosis. Apoptosis of microglial cells was activated by dimerization using the B/B Homodimerizer*. Microglia depletion was measured by FACS analysis. Morphological changes and changes in the distribution of microglia was observed using immunohistological analyses. Cytokine expression was determined by RT-qPCR.

*Studies are presented using iDimerize components. For complete experimental details, please refer to the original publication. The optimal position and copy number of fusion domains varies per study and must be determined empirically.

Key results

  1. Loss of BM-derived microglia enhances inflammatory pathways and accelerates retinal degradation. This also leads to decreased retinal rosettes and an enlarged spleen.
  2. Retinal microglia release inflammatory cytokines-IL-1beta, IL-6, and TNF-alpha. These cytokines increase retinal degeneration.
  3. B/B Homodimerizer does not penetrate the blood-brain barrier, this leads to specific depletion of BM-derived microglia and preserves retinal microglia.

The role of the juxtamembrane region in ΔEGFR nuclear localization The role of the juxtamembrane region in delta EGFR nuclear localization

Research summary

How does ΔEGFR/EGFRvIII translocate to the nucleus and regulate gene expression? The iDimerize Inducible Homodimer System was used to investigate the role of the juxtamembrane region in ΔEGFR nuclear localization. The authors show that nuclear translocation of ΔEGFR is critical to its oncogenic potential, independent of its kinase activity. 

Paper: Gururaj, A. E. et al. Access to the nucleus and functional association with c-Myc is required for the full oncogenic potential of ΔEGFR/EGFRvIII. J. Biol. Chem. 288, 3428-38 (2013). 


Ligand-induced dimerization of deltaEGFR

Advantages of inducible dimerization

  • Induce at any developmental stage
  • Induction is systemic and reversible
  • Specifically activate dimerization-dependent kinase activity

Experimental design

Cell lines stably expressing ΔEGFR with a mutation in the juxtamembrane region (chiΔEGFR-NLS1) were created. In these lines, ΔEGFR with an NLS1 mutation (decreasing nuclear localization) was fused to two copies of the DmrB domain. Dimerization was induced using the B/B Homodimerizer* and the kinase activity and localization of chiΔEGFR-NLS1 were observed.

*Studies are presented using iDimerize components. For complete experimental details, please refer to the original publication. The optimal position and copy number of fusion domains varies per study and must be determined empirically.

Key results

  1. The kinase activity of chiΔEGFR-NLS1 mutants, with decreased nuclear localization, increased upon activation of dimerization. This suggests that kinase activity and nuclear localization of ΔEGFR are independent in glioblastoma cells.
  2. Loss of nuclear localization of ΔEGFR decreases tumor growth in mice. Even when kinase activity is increased by two weeks of dimerization, ΔEGFR tumorigenic effects are attenuated without localization.
  3. Using ChIP-seq, c-Myc binding sites (E-box motifs) were found in a greater number of ΔEGFR binding sites. Downregulation of c-Myc/ΔEGFR genes was found to be coupled with decreased tumorigenic potential.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2023 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • OEM
  • Capabilities and installations
  • OEM enzyme FAQs
  • Enzyme samples for commerical assay developers
  • OEM process
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Partnering & Licensing
  • Vector information
  • Vector document overview
  • Vector document finder
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • cDNA synthesis
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • Antibodies and ELISA
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • Announcements
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Trademarks
  • License statements
  • Quality statement
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Website FAQs

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • COVID-19 research
  • Next-generation sequencing
  • Diagnostic solutions
  • Real-time PCR
  • Stem cell research
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Nucleic acid purification
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • New products
  • Special offers
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • Services & Support
  • OEM
  • Instrument services
  • Stem cell services
  • Gene and cell therapy manufacturing
  • Customer service
  • Sales
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • Partnering & Licensing
  • Vector information
  • OEM
  • Capabilities and installations
  • OEM enzyme FAQs
  • Enzyme samples for commerical assay developers
  • OEM process
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Online tools
  • GoStix Plus FAQs
  • Vector information
  • Vector document overview
  • Vector document finder
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • cDNA synthesis
  • Real-time PCR
  • Nucleic acid purification
  • PCR
  • Cloning
  • Stem cell research
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • APPLICATIONS
  • Molecular diagnostics
  • Vaccine development
  • Pathogen detection
  • Immunotherapy research
  • Cancer research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • Announcements
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • Takara Bio affiliates & distributors
  • Need help?
  • Website FAQs
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us