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Home › Learning centers › Gene function › Inducible systems › Tet-inducible systems › Tet system webinars

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Tet system webinars

We invited leading experts to provide their experiences in the development of the tetracycline inducible system and to discuss key ground-breaking applications that are now possible through the use of this gold-standard technology. Recordings of their webinars are provided below.

In these webinars, Dr. Hermann Bujard and Dr. Manfred Gossen introduce us to Tet technology and other respected researchers describe noteworthy applications of the Tet regulatory systems in basic and applied research. These examples only hint at the breadth of the Tet-based systems that have been described in primary literature and various review articles.


Studying the function of genes by controlling their expression

Studying the function of genes by controlling their expression

In this webinar Dr. h.c. Hermann Bujard, University of Heidelberg, Germany, gives an introduction to the underlying principles of the Tet technology and the concept of gene regulation by tetracyclines. Professor Bujard also discusses the development of the Tet Systems components over time and their advantages as well as giving a review of results obtained by using the technology in transgenic mice. Learn about the underlying principles of the Tet System in the inventor's own words, Tet-On versus Tet-Off, and optimization of the technology.

Watch Now


Controlling gene expression in transgenic mice

Controlling gene expression in transgenic mice

In this webinar Dr. Kai Schönig, Central Institute of Mental Health, Mannheim, Germany, focuses on the use of Tet technology in transgenic mice. Options on how to set up the Tet inducible system in mice will be discussed. Dr. Schönig will also present a number of in vivo applications which demonstrate the potential of this powerful technology. Learn how to establish the Tet System in transgenic mice in vivo applications and on-going developments for in vivo use of tetracycline-inducible expression.

Watch Now


Tet system: selected applications

Tet system: selected applications

In this webinar Dr. Manfred Gossen, BCRT, Berlin, Germany, focuses on applications of particular interest, including conditional RNA interference, the generation of packaging cell lines for virus production and ‘High Throughput Screening’ systems. Learn about conditional RNAi described by one of the co-inventors of the Tet System, Tet system in virus production for added safety, and the high-titer Tet system in high-throughput screening.

Watch Now


Applications of the Tet system: engineering mosquitoes and other pest insects

Applications of the Tet system: engineering mosquitoes and other pest insects

In this webinar Dr. Luke Alphey, Chief Scientist, Oxitec Ltd., UK, focuses on the use of Tet technology in transgenic insects, in the context of a synthetic biology approach to rational development of novel engineered phenotypes. The tet system provides a regulated switch well-suited to a modular design approach. Dr Alphey also discusses his experience of taking such engineered insects through to successful field use. Learn about controlling the spread of dengue fever with engineered mosquitoes, how the Tet system is used to create mosquitoes that cannot fly or breed in the absence of Dox, and fascinating details of a successful field trial conducted in the Cayman Islands.

Watch Now


RNAi mouse models—revolutionizing drug discovery in vivo

RNAi mouse models—revolutionizing drug discovery in vivo

In this webinar Prem K. Premsrirut, PhD, CEO, Mirimus, Inc., talks about RNA interference. RNA interference is a powerful tool for studying gene function, however, the reproducible generation of RNAi tools including RNAi transgenic mice remains a significant limitation. One main hurdle is the identification of potent RNAi triggers, or short hairpin RNAs (shRNAs), that will induce stable and regulated gene silencing. We developed a “Sensor assay” that enables the biological identification of effective shRNAs at large scale and show that our assay reliably identifies potent shRNAs that are surprisingly rare and predominantly missed by existing algorithms. By combining our sensored miR30-based shRNAs with high efficiency ES cell targeting, we developed a fast, scalable pipeline for the production of shRNA transgenic mice. Learn about how to combine the power of RNA interference and Tet-induced expression, “Sensor assay” for large-scale isolation of effective shRNAs, and identify potent shRNAs that are typically missed by existing algorithms.

Watch Now

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Studying the function of genes by controlling their expression

In this webinar Dr. h.c. Hermann Bujard, University of Heidelberg, Germany, gives an introduction to the underlying principles of the Tet technology and the concept of gene regulation by tetracyclines. Professor Bujard also discusses the development of the Tet Systems components over time and their advantages as well as giving a review of results obtained by using the technology in transgenic mice. Learn about the underlying principles of the Tet System in the inventor's own words, Tet-On versus Tet-Off, and optimization of the technology.

Tet-inducible Systems

Controlling gene expression in transgenic mice

In this webinar Dr. Kai Schönig, Central Institute of Mental Health, Mannheim, Germany, focuses on the use of Tet technology in transgenic mice. Options on how to set up the Tet inducible system in mice will be discussed. Dr. Schönig will also present a number of in vivo applications which demonstrate the potential of this powerful technology. Learn how to establish the Tet System in transgenic mice in vivo applications and on-going developments for in vivo use of tetracycline-inducible expression.

Tet-inducible Systems

Tet system: selected applications

In this webinar Dr. Manfred Gossen, BCRT, Berlin, Germany, focuses on applications of particular interest, including conditional RNA interference, the generation of packaging cell lines for virus production and ‘High Throughput Screening’ systems. Learn about conditional RNAi described by one of the co-inventors of the Tet System, Tet system in virus production for added safety, and the high-titer Tet system in high-throughput screening.

Tet-inducible Systems

Applications of the Tet system: engineering mosquitoes and other pest insects

In this webinar Dr. Luke Alphey, Chief Scientist, Oxitec Ltd., UK, focuses on the use of Tet technology in transgenic insects, in the context of a synthetic biology approach to rational development of novel engineered phenotypes. The tet system provides a regulated switch well-suited to a modular design approach. Dr Alphey also discusses his experience of taking such engineered insects through to successful field use. Learn about controlling the spread of dengue fever with engineered mosquitoes, how the Tet system is used to create mosquitoes that cannot fly or breed in the absence of Dox, and fascinating details of a successful field trial conducted in the Cayman Islands.

Tet-inducible Systems

RNAi mouse models—revolutionizing drug discovery in vivo

In this webinar Prem K. Premsrirut, PhD, CEO, Mirimus, Inc., talks about RNA interference. RNA interference is a powerful tool for studying gene function, however, the reproducible generation of RNAi tools including RNAi transgenic mice remains a significant limitation. One main hurdle is the identification of potent RNAi triggers, or short hairpin RNAs (shRNAs), that will induce stable and regulated gene silencing. We developed a “Sensor assay” that enables the biological identification of effective shRNAs at large scale and show that our assay reliably identifies potent shRNAs that are surprisingly rare and predominantly missed by existing algorithms. By combining our sensored miR30-based shRNAs with high efficiency ES cell targeting, we developed a fast, scalable pipeline for the production of shRNA transgenic mice. Learn about how to combine the power of RNA interference and Tet-induced expression, “Sensor assay” for large-scale isolation of effective shRNAs, and identify potent shRNAs that are typically missed by existing algorithms.

Tet-inducible Systems

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

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  • Antibodies and ELISA
  • New products
  • Special offers
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • Spatial omics
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Immune profiling
  • Real-time PCR
  • Great value master mixes
  • Signature enzymes
  • High-throughput real-time PCR solutions
  • Detection assays
  • References, standards, and buffers
  • Stem cell research
  • Media, differentiation kits, and matrices
  • Stem cells and stem cell-derived cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Automated platforms
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
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  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
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