Inducible protein stabilization systems—product selection guide
The ProteoTuner systems use a powerful, unique technology to regulate the quantity of your protein of interest, at the post-translational level. ProteoTuner technology has been successfully used with cytosolic and membrane-bound proteins in a variety of cell types, organisms, and animal models.
| Application | Recommended systems | Comments |
|---|---|---|
| For easy-to-transfect cells | ||
| N-terminal DD tag | ProteoTuner Shield System N ProteoTuner Shield System N (w/ AcGFP1) |
Most of the proteins that Takara Bio has tested show a better destabilization profile when the DD tag is fused to the N-terminus of the protein of interest. Available with or without a built-in green fluorescent protein transfection control. |
| C-terminal DD tag | ProteoTuner Shield System C | A specific DD tag mutant for C-terminal tagging; provides a slightly reduced destabilization activity in the absence of Shield1. |
| For difficult-to-transfect, dividing or nondividing cells | ||
| N-terminal DD tag | Lenti-X ProteoTuner Shield System N Lenti-X ProteoTuner Shield System N (w/ ZsGreen1) |
Most of the proteins that Takara Bio has tested show a better destabilization profile when the DD tag is fused to the N-terminus of the protein of interest. Available with or without a built-in green fluorescent protein transfection control. |
| C-terminal DD tag | Lenti-X ProteoTuner Shield System C | A specific DD tag mutant for C-terminal tagging; provides slightly reduced destabilization activity in the absence of Shield1. |
| For difficult-to-transfect, dividing cells | ||
| N-terminal DD tag | Retro-X ProteoTuner Shield System N Retro-X ProteoTuner Shield System N (w/ZsGreen1) |
Most of the proteins that Takara Bio has tested show a better destabilization profile when the DD tag is fused to the N-terminus of the protein of interest. Available with or without a built-in green fluorescent protein transfection control. |
| Shield1 ligand | ||
| Controlling DD activity | Shield1 | Membrane-permeable small molecule; binds specifically to the DD tag, to inactivate protein degradation. |
| ProteoTuner accessories | ||
| Confirming DD expression | DD Monoclonal Antibody | Detect DD-N and DD-C tagged proteins. |
Inducible systems learning centers
Tet-inducible systems
Tet-On 3G, Tet-One, and Tet-Off systems.
iDimerize systems
Dimerizer ligand-dependent control of protein-protein interactions.
ProteoTuner systems
Shield1 ligand-dependent control of protein stability/degradation.
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