ProteoTuner antibody
The DD Monoclonal Antibody can be use to detect both DD-tagged proteins (N and C versions of ProteoTuner systems) and FKBP12 via Western blot and immunocytochemistry.
Overview
- Mouse monoclonal antibody
- Applicable to ProteoTuner shield systems (both and N and C versions)
- For Western blotting, use a 1:500 dilution
- For immunocytochemistry, use a 1:50 dilution
More Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Control protein stability with Shield1
ProteoTuner systems allow quick, predictable regulation of protein presence or absence in mammalian cells by acting directly on the protein, using a small, cell-permeable synthetic compound. When Shield1 is added to the medium, any fusion protein containing a DD domain can accumulate to detectable levels within 15–20 minutes. Conversely, upon Shield1 removal, the half-time for the protein's degradation can be as short as 30 minutes. This post-translational regulation offers many benefits, from speed and convenience to precise tuning and reversible control.
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