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  • ‹ Back to ProteoTuner protein control systems
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  • Shield1 ligand
Learn more about our tet, ProteoTuner, and iDimerize inducible systems Inducible systems
Citations ProteoTuner citations
Home › Products › Gene function › ProteoTuner protein control systems › ProteoTuner antibody

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Learn more about our tet, ProteoTuner, and iDimerize inducible systems Inducible systems
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ProteoTuner antibody

The DD Monoclonal Antibody can be use to detect both DD-tagged proteins (N and C versions of ProteoTuner systems) and FKBP12 via Western blot and immunocytochemistry.

Cat. # Product Size Price License Quantity Details
631073 DD Monoclonal Antibody 50 uL USD $185.00

The ProteoTuner DD Monoclonal Antibody recognizes the DD-N and DD-C domains, which have been optimized for use as N- and C-terminal fusion tags respectively.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Easily detect ProteoTuner shield system DD fusions with the DD Monoclonal Antibody

Easily detect ProteoTuner shield system DD fusions with the DD Monoclonal Antibody

Easily detect ProteoTuner shield system DD fusions with the DD Monoclonal Antibody. Cell lysates from HeLa cells transiently expressing either DD-AcGFP1 or AcGFP1-DD and HEK 293 cells stably expressing DD-AcGFP1 were analyzed by Western blot using the DD Monoclonal Antibody at a 1:500 dilution. Lane 1: HeLa cells transfected with pDD-AcGFP1 (e.g., DD-N). Lane 2: Negative control (untransfected HeLa cells). Lane 3: HeLa cells transfected with pAcGFP1-DD (e.g., DD-C). Lane 4: Negative control (untransfected HEK 293 cells). Lane 5: HEK 293 cells stably expressing DD-AcGFP1.

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631073: DD Monoclonal Antibody

631073: DD Monoclonal Antibody

Shield1 ligand for protein stabilization Shield1 ligand
Overviews ProteoTuner explained
Selection guides ProteoTuner kit selection guide

Overview

  • Mouse monoclonal antibody
  • Applicable to ProteoTuner shield systems (both and N and C versions)
  • For Western blotting, use a 1:500 dilution
  • For immunocytochemistry, use a 1:50 dilution

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Control protein stability using a small molecule ligand, Shield1

Control protein stability with Shield1

ProteoTuner systems allow quick, predictable regulation of protein presence or absence in mammalian cells by acting directly on the protein, using a small, cell-permeable synthetic compound. When Shield1 is added to the medium, any fusion protein containing a DD domain can accumulate to detectable levels within 15–20 minutes. Conversely, upon Shield1 removal, the half-time for the protein's degradation can be as short as 30 minutes. This post-translational regulation offers many benefits, from speed and convenience to precise tuning and reversible control.

Technology explained Product selection guide

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