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  • ‹ Back to iDimerize inducible protein interaction systems
  • Homodimerization systems
  • Heterodimerization systems
  • Reverse dimerization
  • Regulated transcription
  • Dimerizer ligands
  • In vivo applications
  • Dimerization domain antibodies
Learn more about our tet, ProteoTuner, and iDimerize inducible systems Inducible systems
A/C Heterodimerizer, B/B Homodimeirzer, AP20187, AP21967 Dimerizer ligands
Home › Products › Gene function › iDimerize inducible protein interaction systems › Heterodimerization systems

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Learn more about our tet, ProteoTuner, and iDimerize inducible systems Inducible systems
A/C Heterodimerizer, B/B Homodimeirzer, AP20187, AP21967 Dimerizer ligands

iDimerize Inducible Heterodimer System

Inducible heterodimerization systems

The iDimerize Inducible Heterodimer System can be used to create and control specific interactions between two different proteins. The proteins of interest are fused to the DmrA and DmrC binding domains, respectively, and dimerization is induced by adding the cell-permeant ligand A/C Heterodimerizer (identical to the AP21967 ligand) to the culture medium or by administering it in vivo. Plasmid and lentiviral (Lenti-X) vector formats are available.

The iDimerize Inducible Heterodimer System can be used to create and control specific interactions between two different proteins. The proteins of interest are fused to the DmrA and DmrC binding domains, respectively, and dimerization is induced by adding the cell permeant ligand A/C Heterodimerizer (identical to the AP21967 ligand) to the culture medium or by administering it in vivo. Plasmid and lentiviral (Lenti-X) vector formats are available.

iDimerize Inducible Heterodimer System (with Tet-On 3G technology)

One challenge of ligand-dependent dimerization experiments is that non-ligand-induced dimerization events may occur if the proteins of interest are expressed at high levels. This is especially problematic if the target proteins are membrane-bound, because the local concentrations can increase quickly due to the limited space on the membrane. We’ve combined iDimerize and Tet-On 3G technologies to eliminate these unwanted events. First, use doxycycline (Dox) to optimize the proteins’ expression to physiologically relevant levels. Then induce dimerization by adding the dimerizer ligand to your culture medium.

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Cat. # Product Size Price License Quantity Details
635056 A/C Heterodimerizer 5 x 500 uL USD $558.00

A/C Heterodimerizer is a membrane-permeant ligand that causes heterodimerization of proteins fused to two distinct dimerization domains—DmrA and DmrC—provided in the iDimerize Inducible Heterodimer System (635067) and the iDimerize Regulated Transcription System (Cat. No. 635081). NOTE: The A/C Heterodimerizer is identical to the AP21967 ligand previously supplied in the ARGENT Regulated Homodimerization Kit from ARIAD Pharmaceuticals, Inc.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Molecular weights of iDimerize ligands

Molecular weights of iDimerize ligands
Molecular weights of iDimerize ligands.
635055 A/C Heterodimerizer 5 mg USD $730.00

A/C Heterodimerizer is a membrane-permeant ligand that causes heterodimerization of proteins fused to two distinct dimerization domains—DmrA and DmrC—provided in the iDimerize Inducible Heterodimer System (Cat. No. 635067) and the iDimerize Regulated Transcription System (Cat. No. 635081). NOTE: The A/C Heterodimerizer is identical to the AP21967 ligand previously supplied in the ARGENT Regulated Homodimerization Kit from ARIAD Pharmaceuticals, Inc.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Molecular structure of A/C Heterodimerizer (AP21967)

Molecular structure of A/C Heterodimerizer (AP21967)
Molecular structure of A/C Heterodimerizer (AP21967).

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Molecular weights of iDimerize ligands

Molecular weights of iDimerize ligands
Molecular weights of iDimerize ligands.
635067 iDimerize™ Inducible Heterodimer System Each USD $1095.00

The iDimerize Inducible Heterodimer System lets you control the heterodimerization of two different proteins of interest in live cells via a membrane-permeant compound. The system includes the iDimerize Inducible Heterodimer Vector Set 1, which contains three mammalian expression vectors encoding fusion tags that can be easily added to your proteins of interest. The activity and localization of the resulting chimeric proteins can be controlled by the addition of a small molecule to the cell medium. The system also includes the membrane-permeant compound, A/C Heterodimerizer, required for heterodimerization, and two linear selection markers for hygromycin and puromycin resistance.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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How does iDimerize technology work?

How does iDimerize technology work?

How does iDimerize technology work? A chemical inducer of dimerization, or “dimerizer”, is a cell-permeant organic small molecule with two separate motifs that each bind with high affinity to a specific protein module (Dmr domain) fused onto the protein(s) of interest. Addition of the dimerizer brings the chimeric protein subunits into very close proximity to each other, mimicking the activation of the cellular event that dimerization of interest controls. B/B Homodimerizer induces self-association of two copies of the same protein whereas A/C Heterodimerizer induces association of two different proteins. Conversely, a reverse dimerizer ligand (D/D Solubilizer) will bind to and dissociate a protein that aggregates in its absence.

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Inducible dimerization of heterodimers

Inducible dimerization of heterodimers
Inducible dimerization of heterodimers. Fusion proteins are created which contain the DmrA (green) and DmrC (red) dimerization domains respectively. The two proteins do not interact until the A/C heterodimerizer (AP21967) is added. This cell-permeant ligand induces the fusion proteins to interact, which activates downstream events such as signal transduction pathways.
635079 iDimerize™ Inducible Heterodimer System (with Tet-On® 3G) Each USD $1195.00

License Statement

ID Number  
42 Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 7541446, # 8383364, # 9181556 , European patents EP # 1200607, # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
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TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
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email: info@tetsystems.com
or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 6,998,115; 7,427,394. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
*

The iDimerize Inducible Heterodimer System (with Tet-On 3G) lets you control the expression levels and heterodimerization of two different proteins of interest, encoded on a single vector, in live cells. The system includes the iDimerize Inducible Heterodimer Vector Set 2, which contains a mammalian bidirectional expression vector (pTRE3G-BI-Het1) that allows simultaneous, doxycycline-dependent expression of two proteins of interest, tagged with the DmrA and DmrC domains, respectively, in Tet-On 3G transactivator-expressing cells. The activity and localization of the resulting DmrA- and DmrC-tagged proteins is controlled by the addition of the A/C Heterodimerizer.

The iDimerize Inducible Heterodimer System (with Tet-On 3G) includes vectors and linear selection markers, our highly efficient transfection reagent, Xfect, Tet System Approved FBS, and the A/C Heterodimerizer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635074 Lenti-X™ iDimerize™ Inducible Heterodimer System Each USD $1195.00

License Statement

ID Number  
55 cPPT Element. This product and its use are the subject to one or more of the following U.S. Pat. 8,093,042 and foreign equivalents. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot disclose information, sell or otherwise transfer this product, its components or materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. If the buyer is not willing to accept the limitations of this limited use statement, Takara Bio USA, Inc. is willing to accept return of the product with a full refund. For information on purchasing a license to the DNA-Flap technology for purposes other than research, contact the Transfer of Technology Office, Institut Pasteur, 28 rue du Docteur Roux, 75 724 Paris Cedex 15 (www.pasteur.fr).

The Lenti-X iDimerize Inducible Heterodimer System lets you control the heterodimerization of two different proteins of interest in live cells by adding a small molecule, the membrane-permeant compound A/C Heterodimerizer, to the culture medium. The system includes the Lenti-X iDimerize Inducible Heterodimer Vector Set 1, which contains four lentiviral vectors encoding dimerization tags (DmrA or DmrC, respectively), as well as subcellular localization tags that can be easily fused to your protein of interest. In combination with a lentiviral packaging system, the optimized Lenti-X constructs allow for packaging and delivery of high-titer lentivirus to the widest range of cell types. Using this system, the activity and/or localization of the resulting chimeric protein can be controlled by the addition of A/C Heterodimerizer, which is required for heterodimerization and is also included.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Required Products

Cat. # Product Size Price License Quantity Details
632180 Lenti-X™ 293T Cell Line 1 mL USD $317.00

License Statement

ID Number  
330 This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

The Lenti-X 293T Cell Line is a subclone of the transformed human embryonic kidney cell line, HEK 293, which is highly transfectable and supports high levels of viral protein expression. When transfected with the Lenti-X HTX Packaging System and a lentiviral vector, these cells are capable of producing lentiviral titers as high as >108 ifu/ml, as determined by flow cytometry. The cell line also constitutively expresses the simian virus 40 (SV40) large T antigen.

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Lenti-X 293T Cell Line

Lenti-X 293T Cell Line

Lenti-X 293T Cell Line. Cat. # 632180.

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Clontech's lentiviral packaging system (Panel A) and a lentiviral packaging system from a leading competitor (Panel B) were each used to generate viral supernatants from their respective lentiviral system vectors that were engineered to express the ZsGreen1 fluorescent protein

Clontech's lentiviral packaging system (Panel A) and a lentiviral packaging system from a leading competitor (Panel B) were each used to generate viral supernatants from their respective lentiviral system vectors that were engineered to express the ZsGreen1 fluorescent protein
Clontech's lentiviral packaging system (Panel A) and a lentiviral packaging system from a leading competitor (Panel B) were each used to generate viral supernatants from their respective lentiviral system vectors that were engineered to express the ZsGreen1 fluorescent protein. As little as 10 µl of supernatant from Lenti-X transduced the majority of these HeLa cells, whereas 10 µl of supernatant from the other system transduced only a small percentage of the cells. Transduced cells were quantified by flow cytometry.

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Transduction of neural progenitor cells by Lenti-X lentivirus

Transduction of neural progenitor cells by Lenti-X lentivirus

Transduction of neural progenitor cells by Lenti-X lentivirus. Recombinant lentivirus for expressing ZsGreen1 was produced using Lenti-X virus and used to transduce normal human neural progenitor cells. A single transduced cell is shown under phase contrast microscopy (Panel A) and fluorescence microscopy (Panel B).

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High-titer lentivirus production

High-titer lentivirus production

High-titer lentivirus production. Lenti-X 293T cells were transduced with the indicated volumes (µl) of lentiviral packaging supernatant generated with the Lenti-X Expression System and then selected with puromycin for 9 days to allow the formation of the resistant colonies, which were then stained with crystal violet.

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293T cell line for higher titers

293T cell line for higher titers

293T cell line for higher titers. We used our fourth-generation lentiviral packaging system and one of our pLVX-lentiviral vectors to compare the virus production of the Lenti-X 293T Cell Line to that of two other commonly used HEK 293-based cell lines. Lenti-X 293T cells clearly outperformed the other cell lines—producing over 6X more virus than 293FT cells and up to 30X more virus than the parental HEK 293 cell line.

631275 Lenti-X™ Packaging Single Shots (VSV-G) 16 Rxns USD $825.00

License Statement

ID Number  
259 Patent pending.
47 Portions of this product are covered by several patent applications owned by, or licensed to, GE Healthcare Dharmacon Inc. The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) under these patent rights to perform the viral infection methods using the lentiviral vectors claimed in those patent applications for research purposes solely in conjunction with this product. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, and no other rights are conveyed to the buyer to use the product or components of the product for any other purposes, including without limitation, provision of services to a third party, generation of commercial databases, or clinical diagnostics or therapeutics. This product is sold pursuant to a license from GE Healthcare Dharmacon Inc, and GE Healthcare Dharmacon Inc., reserves all other rights under these patent rights. For information on purchasing a license to the patent rights for uses other than in conjunction with this product or to use this product for purposes other than research, please contact GE Healthcare Dharmacon Inc., LSlicensing @ge.com.
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 6,998,115; 7,427,394. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.

Lenti-X Packaging Single Shots (VSV-G) provide an extremely simple and consistent one-step method for producing high-titer lentivirus. No additional transfection reagent is needed because Lenti-X Packaging Single Shots (VSV-G) consist of pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of VSV-G pseudotyped Lenti-X lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this mixture with your lentiviral vector of choice in sterile water and adding it to 293T cells, e.g., Lenti-X 293T Cells (Cat. No. 632180), in a 10 cm dish.

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The Lenti-X Packaging Single Shots (VSV-G) protocol

The Lenti-X Packaging Single Shots (VSV-G) protocol
The Lenti-X Packaging Single Shots (VSV-G) protocol.

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Consistent, high-efficiency transfections lead to high titers

Consistent, high-efficiency transfections lead to high titers

Consistent, high-efficiency transfections lead to high titers. A lentiviral vector containing the ZsGreen1 gene was packaged according to the Lenti-X single shots protocol in four independent experiments. Briefly, 7 µg of pLVX-ZsGreen1 plasmid was added to each of four Lenti-X single shots; the tubes were vortexed for 20 sec and incubated at room temperature for 10 min. Then, the mixture was added to cultured Lenti-X 293T cells that were approximately 80% confluent. 48 hours after transfection, the cells were imaged by fluorescence microscopy (Panel A, top) and light microscopy (Panel A, bottom). After images were taken, the supernatant was harvested and used infect HT1080 cells for titer determination (Panel B, IFU/ml).

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High-titer virus was produced regardless of the lentiviral vector backbone with Lenti-X packaging single shots

High-titer virus was produced regardless of the lentiviral vector backbone with Lenti-X packaging single shots

High-titer virus was produced regardless of the lentiviral vector backbone with Lenti-X packaging single shots. A CMV ZsGreen1 expression cassette was cloned into several lentiviral vector backbones. These vectors were then packaged into lentivirus using the Lenti-X packaging single shots following the provided protocol. Briefly, 7 µg of pLVX-ZsGreen1 plasmid was added to each of four Lenti-X single shots, and the tubes were vortexed for 20 sec and incubated at room temperature for 10 min. Then, the mixture was added to cultured Lenti-X 293T cells that were approximately 80% confluent. After 48 hours, titer was determined using several methods. To determine infectivity, the supernatant was harvested and used to infect HT1080 cells (Flow Cytometry). Harvested viral supernatants were also analyzed by RT-PCR to quantify viral genome copies (qRT-PCR, Lenti-X qRT-PCR Titration Kit), ELISA to measure p24 (p24 ELISA, Lenti-X p24 Rapid Titer Kit), and by a rapid lentiviral detection method (Lenti-X GoStix).

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A comparison of fourth- and third-generation lentiviral packaging systems

A comparison of fourth- and third-generation lentiviral packaging systems

A comparison of fourth- and third-generation lentiviral packaging systems . Our Lenti-X Packaging single shots utilize a packaging system that consists of five separate components (Panel A), mixed in proprietary proportions for optimized packaging activity. The separation of the gag, pol, and env genes effectively reduces the incidence of RCL (Wu et al., 2000). High levels of expression of essential viral components are driven by the Tet-Off and Tat transactivators, which induce a cascade of expression that results in high titers of lentivirus. The pol gene is fused to vpr to ensure transport of the reverse transcriptase/integrase protein into the recombinant lentiviral particle. Not all vector elements are shown. Other 3rd generation lentiviral packaging systems (Panel B) generate lower titers, do not contain separate gag and pol sequences, and do not use a transactivation cascade mechanism.

*You must be logged in to a Business Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Inducible homodimer systems using AP20187 ligand Induce homodimers
Citations Published studies
Induce transcription using AP21697 dimerizer ligand Regulated transcription

Overview

  • Achieve small-molecule control of cellular events that are usually activated by protein heterodimerization
  • Control the subcellular localization of a target protein
  • A/C Heterodimerizer is identical to the AP21967 ligand
  • For inducible control of transcription factors, use the iDimerize Regulated Transcription System
  • Plasmid and lentiviral (Lenti-X) vector formats are available
  • Previously available from ARIAD Pharmaceuticals, Inc., as the ARGENT Regulated Heterodimerization Kit

More Information

Applications

  • Create and control interactions between two different proteins
  • Create conditional alleles of receptors and signaling molecules
  • Activate cell surface receptors or other signaling proteins
  • Recruit signaling proteins to specific subcellular locations (e.g., the plasma membrane or nucleus)
  • Reconstitute enzyme activity by inducing the interaction of separate polypeptides
  • Functional analysis of a signaling pathway in multiple cell types
  • Inducible protein dimerization in animal models and cultured cell lines

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Related links

  • Technology overview
  • iDimerize in vivo protocol
  • Nomenclature changes
  • Published studies
  • Homodimerization systems
  • Heterodimerization systems
  • Reverse dimerization
  • Regulated transcription
  • Dimerizer ligands
  • Dimerization domain antibodies
  • iDimerize journal club

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Alzheimer's disease research

Alzheimer's disease research

Alzheimer's disease (AD) affects ~5.8 million adults and is the sixth leading cause of death in the US alone. Over 400 AD clinical trials are currently under way, investigating the possibilities of immunotherapy, our microbiome, and inflammation as prospective therapeutic targets. We have a variety of best-in-class products that can help your AD research move forward.

See how our best-in-class solutions can help to advance your AD research

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

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Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

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That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

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