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Gene function

Selectable mammalian expression vectors

Selectable mammalian expression vectors

The pIRES selection vectors pIRESpuro3, pIREShyg3, pIRESneo3, and pIRESbleo3 each contain a multiple cloning site located upstream of a selection marker expressing IRES. Because your gene of interest and a selection marker are translated from a single RNA, you can be confident that nearly 100% of the colonies resistant to puromycin, hygromycin, G418, or bleomycin also express your protein of interest.

The pIRES selection vectors pIRESpuro3, pIREShyg3, pIRESneo3, and pIRESbleo3 each contain a multiple cloning site located upstream of a selection marker expressing IRES. Because your gene of interest and a selection marker are translated from a single RNA, you can be confident that nearly 100% of the colonies resistant to puromycin, hygromycin, G418, or bleomycin also express your protein of interest. All IRES selection vectors contain a partially disabled IRES which reduces the efficiency of translation initiation for the selection marker relative to that of the cloned gene and allows preferential selection of cells expressing high levels of your protein of interest.

The pBApo series of vectors are mammalian expression vectors for constitutive expression of your gene of interest from either a CMV or EF1-alpha promoter. The antibiotic resistance marker, either puromycin or neomycin, is expressed separately from an SV40 promoter. pBApo-CMV lacks a selectable marker.

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Cat. # Product Size Price License Quantity Details
3242 pBApo-CMV DNA 20 ug $255.00

pBApo-CMV is a plasmid for gene expression in mammalian cells. This vector has a promoter from cytomegalovirus (CMV IE promoter) and polyA signal from herpes simplex virus thymidine kinase. A target gene can be cloned into multiple cloning site.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3240 pBApo-CMV Neo DNA 20 ug $268.00

pBApo-CMV Neo is a plasmid for gene expression in mammalian cells. This vector has a promoter from cytomegalovirus (CMV IE promoter) and polyA signal from herpes simplex virus thymidine kinase. A target gene can be cloned into multiple cloning site and, after transfection, stable integration of the vector can be selected for using G418.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3241 pBApo-CMV Pur DNA 20 ug $268.00

pBApo-CMV Pur is a plasmid for gene expression in mammalian cells. This vector has a promoter from cytomegalovirus (CMV IE promoter) and polyA signal from herpes simplex virus thymidine kinase. A target gene can be cloned into multiple cloning site and, after transfection, stable integration of the vector can be selected for using puromycin.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3243 pBApo-EF1alpha Neo DNA 20 ug $268.00

pBApo-EF1a Neo is a plasmid for gene expression in mammalian cells. This vector has a promoter from the human EF-1 alpha promoter and polyA signal from herpes simplex virus thymidine kinase. A target gene can be cloned into multiple cloning site and, after transfection, stable integration of the vector can be selected for using G418.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3244 pBApo-EF1alpha Pur DNA 20 ug $268.00

pBApo-EF1a Pur is a plasmid for gene expression in mammalian cells. This vector has a promoter from the human EF-1 alpha promoter and polyA signal from herpes simplex virus thymidine kinase. A target gene can be cloned into multiple cloning site and, after transfection, stable integration of the vector can be selected for using puromycin.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components

Required Products

Cat. # Product Size Price License Quantity Details
740490.250 NucleoSpin® Plasmid Transfection-grade 250 Preps $355.00

NucleoSpin Plasmid Transfection-grade is a fast miniprep kit that uses a new technology developed by Macherey-Nagel to reduce the level of endotoxins co-purified during plasmid preparations from bacterial lysates. Since endotoxins interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection.

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631306 Puromycin 100 mg $195.00

Puromycin is used to select for cell lines that have been transformed by vectors that express puromycin-N-acetyl-transferase (pac), e.g., pPUR (Cat. No. 631601). (3'-[α-Amino-p-methoxy-hydrocinnamamido]-3'-deoxy-N, N-dimethyladenosine) dihydrochloride. This is the 100 mg format. For  the 25 mg format see Cat. # 631305.

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631317 Xfect™ Transfection Reagent 100 Rxns $218.00

Xfect is a transfection reagent that creates biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA into mammalian cells. Transfections can be carried out entirely in the presence of serum.

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631622 pIRESbleo3 Vector 20 ug $426.00

The pIRESbleo3 Vector is designed for the efficient production of stable cell lines expressing recombinant proteins in mammalian cells. This vector contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV) which permits both the gene of interest and the bleomycin selection marker to be translated from a single mRNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631620 pIREShyg3 Vector 20 ug $426.00

pIREShyg3 Vector is designed for the efficient production of stable cell lines expressing recombinant proteins in mammalian cells. This vector contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV), which permits both the gene of interest and the hygromycin B selection marker to be translated from a single mRNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Required Products

Cat. # Product Size Price License Quantity Details
631309 Hygromycin B 1 g $222.00

For the selection and maintenance of bacterial and eukaryotic cells which have been stably transfected with the E. coli hygromycin resistance gene (hyg or hph). Susceptibility of cells to hygromycin B varies with cell type, proliferative activity, and other parameters; therefore, we suggest performing a full dose-response curve for each cell system used.

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740490.250 NucleoSpin® Plasmid Transfection-grade 250 Preps $355.00

NucleoSpin Plasmid Transfection-grade is a fast miniprep kit that uses a new technology developed by Macherey-Nagel to reduce the level of endotoxins co-purified during plasmid preparations from bacterial lysates. Since endotoxins interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection.

Documents Image Data
631317 Xfect™ Transfection Reagent 100 Rxns $218.00

Xfect is a transfection reagent that creates biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA into mammalian cells. Transfections can be carried out entirely in the presence of serum.

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631621 pIRESneo3 Vector 20 ug $426.00

pIRESneo3 Vector is designed for the efficient production of stable cell lines expressing recombinant proteins in mammalian cells. This vector contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV), which permits both the gene of interest and the neomycin selection marker to be translated from a single mRNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631619 pIRESpuro3 Vector 20 ug $426.00

pIRESpuro3 Vector is designed for the efficient production of stable cell lines expressing recombinant proteins in mammalian cells. This vector contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV), which permits both the gene of interest and the puromycin selection marker to be translated from a single mRNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Overview

  • pIRES vectors include either puromycin, hygromycin, G418, or bleomycin resistance for selection
  • pBApo vectors include either neomycin or puromycin resistance for selection
  • Allow for stable product expression in mammalian cells

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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