- Long ssDNA for knockins
- Knockin screening kit
- Genome-wide sgRNA library system
- Recombinant Cas9 protein
- GMP recombinant Cas9
- Mutation detection kits
- In vitro transcription and screening kits
- Cas9-sgRNA gesicle production
- Cas9 antibodies
- Plasmid systems
- Lentiviral systems
- AAV systems
- RNA transfection
- Genotype confirmation kit
- Indel identification kit
- Cre recombinase
Guide-it Mutation Detection Kit
The Guide-it Mutation Detection Kit contains all the reagents needed for PCR-based identification of insertions or deletions generated in the genome of cells treated with engineered nucleases such as zinc-finger nucleases (ZFNs), transcription-activator-like effector nucleases (TALENs), or CRISPR/Cas9. The first step is the amplification of the putative target sequence directly from cells.
The Guide-it Mutation Detection Kit contains all the reagents needed for PCR-based identification of insertions or deletions generated in the genome of cells treated with engineered nucleases such as zinc-finger nucleases (ZFNs), transcription-activator-like effector nucleases (TALENs), or CRISPR/Cas9. The first step is the amplification of the putative target sequence directly from cells. This kit uses Terra PCR Direct Polymerase Mix and Buffer, so there is no need to extract genomic DNA from your cell population prior to amplification of your target sequence. The amplicon is then melted and hybridized to form the mismatched targets for cleavage by the Guide-it Resolvase. Agarose gel electrophoresis can be used to visualize the cleavage products.
- Simple method to identify insertions or deletions in mammalian cells
- Faster and more efficient than the surveyor assay; amplify directly from your target cells
- Complete kit: Guide-it Resolvase, Terra PCR Direct Polymerase, and all buffers included
- Value: 100 reactions per kit; also available in a 25-reaction size for small-scale genome editing projects
- Perfect for confirming sequence changes downstream from Cas9/CRISPR, TALENs, or zinc-finger nuclease studies
- Confirmation of mutations caused by genome editing technologies, including CRISPR/Cas9, TALENs, or zinc-finger nucleases
- Detecting insertions and deletions in mammalian genomic DNA
- Extraction Buffer 1
- Extraction Buffer 2
- Guide-it Resolvase
- Terra PCR Direct Polymerase Mix (1.25 U/µl)
- 2X Terra PCR Direct Buffer (with Mg2+, dNTP)
- PCR-Grade Water
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Mutation detection kit tech note
Read about the Guide-it Mutation Detection Kit and see data comparing it with a standard CEL nuclease assay for detection of CRISPR/Cas9-induced mutations in mammalian cells.See data Gene editing product finder
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SNP engineering application note
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CRISPR/Cas9 gesicles overview
Learn about Guide-it CRISPR/Cas9 Gesicle Production System components and workflow.
CRISPR library screening webinar
Watch this webinar to learn how you can perform genome-wide lentiviral sgRNA screens easily.
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Watch a webinar on how to choose the right HDR template for knockin experiments.
Guide-it SNP Screening Kit FAQs
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